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Anti-adhesion culture medium

A culture medium and microbial technology, applied in the field of cultivating microorganisms, can solve problems such as unfavorable growth, release of products, and rapid development of biofilms

Pending Publication Date: 2020-07-07
FERMENTALG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] These methods are difficult to implement because it is difficult to release the correct amount of product according to the growth and there is a risk of the biofilm developing too quickly
Once the biofilm has formed, the product is less effective
Also, the effectiveness of these chemical techniques is based on the death of the microalgae, thus detrimental to their growth

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0064] Two microalgae cultures were performed, one control without flocculant and one with flocculant FeCl 3 .

[0065] Materials and methods

[0066] microalgae

[0067] The microalgae used in this example were from the species Chlorella sorokiniana.

[0068] culture medium

[0069] BG-11 growth medium (UTEX) was used.

[0070] Culture conditions

[0071] Inoculate the column with 0.5 g / L microalgae. The temperature was kept constant at 30°C. Brightness set to 500μEinstein / m 2 / s. Up to 160 hours (control) and 189 hours (FeCl 3 ), the pH stabilized at 6.3, then without adjustment, the pH was raised to 8 to stabilize at that level until the end of the experiment. It was chosen not to adjust the pH further because high pH increases the ability of microalgae to adhere to the wall.

[0072] Biomass recycling

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PUM

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Abstract

The present invention relates to a method for culturing microorganisms, in particular protists, carried out in such a way as to prevent the microorganisms from adhering to the walls of the reactors inwhich the culture is performed.

Description

[0001] field of invention [0002] The present invention relates to a method for the cultivation of microorganisms, in particular protists, in such a manner that said microorganisms are prevented from adhering to the walls of the reactor in which the cultivation takes place. [0003] Background of the invention [0004] During long-term culture (days to months), microorganisms tend to adhere to the walls. This phenomenon is known as fouling and is well known. It has a very detrimental effect on both the productivity of the culture and the life of the equipment which must be cleaned regularly. Cells that adhere to the walls of the bioreactor reduce the amount of light that penetrates the interior, they can die from lack of resources (nutrients, oxygen, etc.), leading to the development of necrophagous organisms. [0005] Some materials produce less adhesion than others, but none of them overcome the disadvantages mentioned above. [0006] The salinity of the medium in which t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12N1/12
CPCC12M21/02C12M39/00C12N1/12C12N1/10C12N5/0018C12N2500/22C12N2500/24C12N2533/30
Inventor P·布迪耶
Owner FERMENTALG
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