77 results about "Salmonella frintrop" patented technology

The invention discloses an attenuated recombinant bacterium, a preparation method thereof, application, and a tumor targeted medicine. RelA and spoT mutant salmonella typhimurium is adopted as a carrier, and a recombinant plasmid for encoding a ClyA gene is transferred into the relA and spoT mutant salmonella typhimurium so as to obtain the attenuated recombinant bacterium. The preparation methodcomprises the steps of constructing the recombinant bacterium, preparing the relA and spoT mutant salmonella typhimurium, and guiding. According to the attenuated recombinant bacterium, through the plasmid carrying encoding cytolysin, ClyA protein is over-expressed after a bacterium targeting tumor, tumor stroma cells and tumor cells are effectively killed through destroying the completeness of acell membrane, and the attenuated recombinant bacterium can be applied to preparing the tumor targeted medicine.

The invention discloses a salmonella pullorum and mycoplasma synoviae double-plate agglutination antigen as well as a preparation method and application thereof, and the preparation method comprises the following steps: respectively carrying out resuscitation passage on salmonella pullorum C79-1 and mycoplasma synoviae GX11-T to prepare seed bacterial liquid, inoculating the seed bacterial liquid into a corresponding culture medium, carrying out enlarged culture, harvesting, respectively inactivating by a formaldehyde solution, concentrating, mixing the two bacterial liquids according to a certain volume ratio, adding a crystal violet solution with the final concentration of 3% by mass fraction for dyeing and glycerol with the volume fraction of 10%, uniformly mixing, and subpackaging, thereby obtaining the product. The technological method is simple and reasonable, low in cost and good in stability, the prepared double-plate agglutination antigen can detect two epidemic disease antibodies at the same time through one-time reaction, the advantages of being high in sensitivity, rapid in diagnosis, high in specificity, easy to observe the agglutination effect and the like are achieved, the detection time and the detection cost are remarkably saved, and the detection efficiency is improved. The product can be clinically used for rapidly detecting the two antibodies of the pullorum disease and the mycoplasma synoviae, diseased chickens are eliminated, and disease purification is achieved.