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Enterococcus faecium YQH2 and application thereof

A technology of Enterococcus faecium and Salmonella, which is applied to Enterococcus faecium YQH2 and its application in chicken breeding, can solve the problems of unclear regulation mechanism of the intestinal tract of livestock and poultry, and achieve the maintenance of intestinal mucosal stability, increase the length, improve damage effect

Active Publication Date: 2021-05-14
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, little is known about the regulation mechanism of probiotics on intestinal stem cells of livestock and poultry animals, but the regulation mechanism of Enterococcus faecium as a probiotic in the intestine is still unclear, especially the regulation mechanism of intestinal stem cells to be further explored

Method used

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  • Enterococcus faecium YQH2 and application thereof
  • Enterococcus faecium YQH2 and application thereof
  • Enterococcus faecium YQH2 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The cultivation and identification of embodiment 1 bacterial strain

[0030] 1. Culture of strains

[0031] Collect the feces of healthy chickens, place them in a conical flask filled with glass beads and oscillate fully, and after standing at room temperature for 2-3 minutes, take 100 μl of the suspension and coat MRS agar medium, streak on the MRS solid medium, and place Incubate overnight in a 37°C incubator for 16 hours. When the colony grows to an appropriate size, select a typical colony and place it in MRS liquid medium for overnight culture. Then inoculate again according to 2% (v / v) for secondary activation, which is convenient for use in subsequent experiments.

[0032] At the same time, Salmonella typhimurium SL1344 (a gift from Professor Liu Shulin, Department of Microbiology, Peking University) was streaked on the LB solid medium with streptomycin, and cultured in a 37°C incubator for 16 hours. Pick typical colonies and place them in LB liquid medium wit...

Embodiment 2

[0037] Example 2 The protective effect of Enterococcus faecium YQH2 on intestinal mucosal tissue

[0038] Using the broiler intestinal mucosa tissue after various treatments in Example 1, carrying out pathological changes after HE staining, it was found that Enterococcus faecium YQH2 can repair the damage of intestinal villi morphology and structure, and reduce the pathological score caused by Salmonella typhimurium damage to 1 point ( figure 2 A-B), increasing the length of small intestinal villi (increased by 34.97%) and the depth of small intestinal crypts (increased by 74.34%) ( figure 2 C); At the same time, it was found that Enterococcus faecium YQH2 can significantly reduce the colonization of Salmonella typhimurium (decreased by 66.67%), and ELISA kit detection found that Enterococcus faecium YQH2 can reduce the concentration of TNF-α, slow down the inflammatory response and the degree of infection ( figure 2 D-E).

Embodiment 3

[0039] Example 3 Enterococcus faecium YQH2 activates the Wnt signaling pathway to promote the proliferation of intestinal stem cells and repair the morphology of intestinal mucosa

[0040] Infection with Salmonella typhimurium damages the intestinal epithelium and disrupts the intestinal mucosal barrier, but the proliferation of the intestinal epithelium after injury is essential for wound healing. The integrity of the intestinal epithelium depends on the proliferation and renewal of intestinal stem cells, and the Wnt / β-catenin signaling pathway regulates the proliferation of intestinal stem cells. Therefore, we detected the multiplication of chicken intestines after various treatments in Example 1, and found that Salmonella typhimurium can cause PCNA in crypts. + Decreased number of cells, and Enterococcus faecium YQH2 can increase PCNA in intestinal crypts of chicks after infection + The proportion of cells (increased by 49.99%) ( image 3 A-B); at the same time, whether i...

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Abstract

The invention discloses an enterococcus faecium YQH2, and further discloses a probiotic preparation. The probiotic preparation comprises the enterococcus faecium YQH2. The invention also discloses application of the enterococcus faecium YQH2 or the probiotic preparation in breeding of poultry animals. The invention also discloses application of the enterococcus faecium YQH2 or the probiotic preparation in preparation of medicines for repairing intestinal injury or enhancing intestinal immunity. The invention further discloses application of the enterococcus faecium YQH2 or the probiotic preparation in preparation of amedicines for inhibiting salmonella typhimurium infection. The enterococcus faecium YQH2 disclosed by the invention can inhibit the growth of pathogenic bacteria. The enterococcus faecium YQH2 can improve the crypt depth of salmonella infection and increase of an inflammatory factor level, stimulates proliferation of intestinal mucosa epithelial cells and has the potential of maintaining intestinal mucosa barriers.

Description

technical field [0001] The invention belongs to the technical field of biological control, and specifically relates to a shit Enterococcus YQH2 and its application in chicken farming, oral administration shit Enterococcus YQH2 can enhance intestinal immune function, promote intestinal epithelial proliferation, have the potential to maintain intestinal barrier homeostasis, and can protect chickens infected with Salmonella typhimurium. Background technique [0002] Enterococcus faecium (Enterococcus Faecium) belongs to the genus Enterococcus, is a Gram-positive bacterium, and is part of the normal flora in the intestinal tract of humans and animals. As a probiotic, Enterococcus faecium has been included in the "Catalogue of Feed Additives" of the Ministry of Agriculture. [0003] Pathogenic bacteria, viruses, chemical substances, environmental factors, etc. can cause intestinal inflammation in livestock and poultry animals, seriously threaten the health of livestock and poult...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/741A61K9/19A61K9/08A61P1/00A61P31/04A61P37/04C12R1/01
CPCC12N1/20A61K35/741A61K9/19A61K9/08A61P1/00A61P37/04A61P31/04
Inventor 庾庆华贾俊鹏谢霜赵珈翊王敏娟
Owner NANJING AGRICULTURAL UNIVERSITY
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