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Preparation method and test method of bacteriophage mixed preparation for killing salmonella

A technology of salmonella and phage, applied in the field of veterinary bactericidal preparations, can solve the problems of potential value to be developed and applied, achieve good bactericidal effect, reduce the generation of bacterial resistance, and improve the effect of bactericidal ability

Pending Publication Date: 2020-07-24
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, the potential value of phage preparations remains to be developed and applied

Method used

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  • Preparation method and test method of bacteriophage mixed preparation for killing salmonella
  • Preparation method and test method of bacteriophage mixed preparation for killing salmonella
  • Preparation method and test method of bacteriophage mixed preparation for killing salmonella

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Embodiment Construction

[0028] The technical solutions in the embodiments of the present invention are clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, rather than all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0029] A kind of preparation and test method of the phage mixed preparation of killing Salmonella of the present invention, specifically realize by following steps:

[0030] 1. Induction and identification of mild Salmonella phage

[0031] 1.1 Induction and identification of Salmonella phage

[0032] In this study, different serotypes of Salmonella were induced by Mitomycin C reagent method, the final concentration was 1 μg / mL, after 3 hours of dark induction, the induced solution was filtered with a 0.22 μm filter, and the filtrate number...

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Abstract

The invention discloses a preparation method and a test method of a bacteriophage mixed preparation for killing salmonella, and relates to the technical field of veterinary sterilization preparations.The preparation method disclosed by the invention comprises the following steps: s01, performing induction and identification of mild salmonella bacteriophage; s02, purifying the salmonella bacteriophage; s03, storing the suspension; and s04, preparing phage cocktail; the test method comprises the following steps: t1, preparing a liquid culture medium; t2, diluting a bacterial liquid; t3, performing mixing preparation; and t4, performing reference test. For the salmonella with different concentrations, the preparation has a good bactericidal effect, can improve the bactericidal ability of thesalmonella compared with antibiotics and reduce the generation of bacterial resistance, and is a mixed preparation applied to environmental sterilization instead of the antibiotics.

Description

technical field [0001] The invention belongs to the technical field of veterinary bactericidal preparations, in particular to a preparation method of a phage mixture preparation for killing salmonella and a test method for a phage mixture preparation of killing salmonella. Background technique [0002] Salmonella (Salmonella) is an important type of intestinal pathogenic bacteria, which can cause diarrhea, enteritis, sepsis and abortion in animals, and can also infect humans. It is the most important pathogen that causes food poisoning in humans. Hygiene means a lot. Salmonella includes 2 species, Salmonella enterica and Salmonella Bongor, and 7 subspecies and more than 2,500 serotypes. Among them, 292 serotypes have been reported in my country, most of which are pathogenic. In my country, food poisoning caused by Salmonella is at the top of the list. In addition, it is particularly serious for pig and poultry breeding, which can cause diseases such as hog cholera, swine t...

Claims

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Application Information

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IPC IPC(8): C12N7/00C12N7/02C12Q1/18A61K35/76A61P31/04A01N63/40A01P1/00C12R1/92C12R1/42
CPCC12N7/00C12Q1/18A61K35/76A61P31/04A01N63/00C12N2795/10321C12N2795/10351C12N2795/10331C12N2795/10332G01N2333/255
Inventor 马喆范亚娟
Owner NANJING AGRICULTURAL UNIVERSITY
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