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Compound microecological preparation as well as preparation and application thereof

A technology of compound microecology and preparations, applied in biochemical equipment and methods, applications, microorganisms, etc., can solve problems such as antibiotic resistance and environmental problems that are difficult to solve, achieve excellent preventive effects, improve immunity, and inhibit growth. Effect

Inactive Publication Date: 2018-12-18
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, at present, there is no effective preventive measure for the widespread occurrence of pullorum salmonellosis in poultry, and the abuse of antibiotics has led to intractable drug resistance and environmental problems. The development of safe, green, stable and effective new It is urgent to replace antibiotics with biological feed additives

Method used

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  • Compound microecological preparation as well as preparation and application thereof
  • Compound microecological preparation as well as preparation and application thereof
  • Compound microecological preparation as well as preparation and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0047] (1) Lactobacillus plantarum subspecies Zhang-LL and Lactobacillus paracasei KL1 preserved in glycerol tubes at -80°C were respectively inoculated at 2% in 5 mL of improved MRS medium, cultured at 37°C for 12 h, and subcultured for two Get activated bacteria for the first time;

[0048] Then, the activated strains were inoculated in 50 mL of the modified MRS medium at 2%, respectively, and cultured at 37° C. for 12 hours to obtain an expanded culture solution.

[0049] (2) The expanded culture solution was inoculated into 1000 mL of sterilized modified MRS medium according to the inoculum amount of 2% to 3%, and cultured at 37° C. for 14 to 16 hours to obtain a fermentation broth.

[0050] (3) Centrifuge the obtained fermentation bacteria liquid at 8000r / min for 20min at 4°C, discard the supernatant under sterile conditions, and collect the bacteria sludge;

[0051] To the collected Zhang-LL and KL1 sludges, respectively add lyoprotectant of 1 / 10 of the original volume ...

experiment example 1

[0064] 1. Antibacterial test in vitro

[0065] The Zhang-LL freeze-dried bacterial powder of Example 1, the KL1 freeze-dried bacterial powder and the compound microecological preparation were used as experimental materials to carry out in vitro antibacterial experiments, as follows:

[0066] Add Zhang-LL freeze-dried bacteria powder, KL1 freeze-dried bacteria powder, and compound probiotics (the mass ratio of Zhang-LL freeze-dried bacteria powder to KL1 freeze-dried bacteria powder is 1:1) at a mass volume ratio of 2‰ In 100mL of modified MRS medium, dissolve and mix evenly and incubate at 37°C for 24h to obtain Zhang-LL strain, KL1 strain fermentation broth, and Zhang-LL strain and KL1 strain mixed fermentation broth. The above-mentioned each bacterial strain fermented liquid is to be tested solution, carries out following experiment:

[0067] Put the Oxford cup vertically on the cell concentration of Salmonella pullorum pullorum (CVCC523) at 10 7 On a CFU / mL plate, take 10...

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Abstract

The invention provides a compound microecological preparation as well as preparation and application thereof. A preparation method of the compound microecological preparation comprises the steps of fermenting and culturing lactobacillus plantarum subspecies Zhang-LL and lactobacillus paracasei KL1 to obtain fermentation liquor, and separately centrifuging the fermentation liquor to obtain bacterial mud; separately mixing the two types of bacterial mud with a freeze-drying protective additive, then carrying out freeze-drying treatment, and compounding to obtain the compound microecological preparation. The compound microecological preparation provided by the invention has a strong in-vitro salmonella inhibition effect, has an excellent preventive effect on salmonella pullorum disease, can inhibit the growth of salmonella enterica, maintains the intestinal micro-ecological balance of chicks, and has a good repair effect for the diseased tissues of the infected chicks; the compound microecological preparation can improve the growth performance of the chicks infected with the salmonella pullorum disease, improves the immunity of the chicks, and improves the anti-stress ability and antioxidant capacity of the organism; an effective method is provided for preventing the occurrence of chicken salmonellosis, and a thought is also provided for the development of a novel biological feedadditive.

Description

technical field [0001] The invention relates to the field of microbial fermentation products, in particular to a compound microecological preparation and its preparation and application. Background technique [0002] Salmonella (Salmonella) is a class of Gram-negative enterobacteriaceae belonging to the family Enterobacteriaceae. It has no capsule, spores, and flagella all over the body. It can adsorb on the surface of host cells and mainly reside in the intestines of humans and other warm-blooded animals. Salmonella can not only cause disease and death of poultry and cause serious economic losses, but also poultry products contaminated by Salmonella will seriously endanger human health. [0003] The poultry industry has become an important source of income for many countries. Commercial farming makes poultry susceptible to environmental stress and disease, and leads to deterioration of the growing environment, resulting in serious economic losses. Since the late 1940s, an...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P31/04A61P37/04A61P39/00A61P39/06A23K10/18C12R1/25C12R1/225
CPCA23K10/18A61K35/747A61P31/04A61P37/04A61P39/00A61P39/06C12N1/20
Inventor 刘慧张红星谢远红李嘉懿
Owner BEIJING UNIV OF AGRI
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