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Method for detecting salmonella

A Salmonella detection method technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, resistance to vector-borne diseases, etc., can solve the problems of low sensitivity, false positives, false negatives, etc., and achieve high sensitivity and accuracy Good, low accuracy effect

Pending Publication Date: 2020-11-20
FOSHAN UNIVERSITY
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AI Technical Summary

Problems solved by technology

However, the existing colloidal gold test strips are prone to false positives, false negatives, and low sensitivity when detecting Salmonella. Therefore, it is urgent to establish a method that can quickly and accurately detect Salmonella to solve the above problems.

Method used

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  • Method for detecting salmonella
  • Method for detecting salmonella

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Embodiment 1

[0030] 1. Preparation of colloidal gold test strips

[0031] The colloidal gold test strip comprises: support plate, sample pad, colloidal gold pad, nitrocellulose membrane and water-absorbent pad, and described sample pad, colloidal gold pad, nitrocellulose membrane and water-absorbent pad are on the support plate according to The sequence is fixed sequentially, the colloidal gold pad contains colloidal gold-labeled Salmonella antibody, and the nitrocellulose membrane is provided with a detection line and a quality control line.

[0032] The construction step of described colloidal gold test strip comprises:

[0033] (a) Pretreatment of the sample pad: Soak the glass fiber membrane in the sample pad buffer ((1% Triton, 1% BSA, 2% glucose, 50mM boric acid, pH 8.0) for 30min, then dry overnight on a plastic rack, After drying, cut the sample pad into a size of about 17mm×30cm, and store it dry;

[0034] (b) Preparation of colloidal gold-labeled Salmonella antibody: colloidal ...

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Abstract

The invention provides a method for detecting salmonella. The method comprises the following steps: (1) taking nucleic acid of a sample to be detected as a template, and carrying out amplification bya loop-mediated isothermal amplification technique by using specific primers of the salmonella, so as to obtain an amplified product; (2) under mediation of specific crRNA, subjecting the amplified product obtained in the step (1) to a cracking reaction by using a CRISPR system, so as to obtain a cracked product; and (3) carrying out chromogenic detection on the cracked product obtained in the step (2) by using a colloidal gold test paper strip. The method has very good sensitivity and accuracy.

Description

technical field [0001] The invention belongs to the technical field of food detection, and in particular relates to a method for detecting salmonella. Background technique [0002] Salmonella is a zoonotic pathogen that seriously threatens food safety and animal production safety. In the world, Salmonella is the chief culprit of bacterial food poisoning. Salmonella, which has many hosts in nature, not only endangers human health, but also causes diseases to various livestock and poultry. The infected objects are regardless of age group, and the most susceptible is Young animals, especially piglets, calves, suckling sheep, foals, etc. Susceptible livestock and poultry take pigs and chickens as examples. Salmonellosis in pigs is paratyphoid in piglets. The speed of onset causes different symptoms. There are many kinds of salmonellosis in chickens, common ones are pullorum, typhoid fever, paratyphoid fever, and Arizonanella. Among the above-mentioned diseases, pullorum and c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6804C12Q1/689C12Q1/10
CPCC12Q1/6804C12Q1/689C12Q2531/119C12Q2521/327C12Q2565/625Y02A50/30
Inventor 曾令文易志健梁琼心吴靖华甄俊杰
Owner FOSHAN UNIVERSITY
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