31 results about "Salmonella antibody" patented technology

A fluorescent fiber-optic biosensor system using ultrasonic concentration of particles and cells for the detection of Salmonella typhimurium. A biosensor test chamber serves as an ultrasonic standing-wave cell that allows microspheres or cells to be concentrated in parallel layers or in a column along the axis of the cell. A fiber probe along the axis delivers laser excitation to fluorescent-labeled antibodies of Salmonella and collects the fluorescent signal. The Salmonella-antibody complexes are moved acoustically to the axis of the cell, increasing the fluorescent signal. Alternatively, the Salmonella-labelled antibody complexes attach to unlabeled antibodies that have been immobilized on the surface of polystyrene microspheres. This entire structure can be manipulated acoustically and the increase in the fluorescent signal, which can be an order of magnitude, indicates the presence of Salmonella.

The invention relates to test paper for salmonella and a preparation method of the test paper. The test paper for the salmonella comprises a bottom plate, the bottom plate is provided with a first end and a second end and provided with a sample pad, a colloidal gold pad, a nitrocellulose membrane and a water absorption pad in sequence from the first end to the second end, the colloidal gold pad is provided with salmonella antibodies marked with colloidal gold, a detection line and a comparison line are further formed on the nitrocellulose membrane, the detection line is prepared from a salmonella antibody membrane capable of being combined with the salmonella, and the comparison line is prepared from a goat anti-rabbit antibody membrane capable of being combined with the salmonella antibodies.

The invention discloses a pullorum disease antibody latex agglutination negative selection detection kit, comprising a salmonella surface protein InvJ recombinant protein sensitized latex reagent and a secreted protein SopA C-terminal recombinant protein sensitized latex reagent, wherein the InvJ recombinant protein sensitized latex reagent can rapidly detect a salmonella antibody by virtue of a latex agglutination test, the SopA C-terminal recombinant protein sensitized latex reagent can detect serotype salmonella antibodies except for the pullorum disease by virtue of the latex agglutination test, and the combination of the two reagents is used for negative selection of a pullorum disease antibody positive blood or serum sample. The invention also discloses a preparation method of salmonella surface protein InvJ recombinant protein and secreted protein SopA C-terminal recombinant protein sensitized latex reagents. The pullorum disease antibody latex agglutination negative selection detection kit disclosed by the invention has the advantages of stable antigen, strong specificity and high sensitivity, and solves the problem of antigen instability caused by potential variability and changed culture conditions of an existing pullorum disease agglutination antigen production strain.

The invention relates to a method for detecting bacteria. The method comprises the steps as follows: coupling magnetic nanospheres and fluorescent nanospheres with mouse typhus salmonella antibodies so as to obtain mouse typhus salmonella-targeted immunologic magnetic spheres and immunologic fluorescent spheres, and adding the mouse typhus salmonella-targeted immunologic magnetic spheres and immunologic fluorescent spheres into a detection system so as to realize magnetic capture and fluorescent labeling to the mouse typhus salmonella simultaneously. About 10 CFU / mL of the mouse typhus salmonella can be detected through observation of a fluorescent microscope; and a good quantitative relation is obtained through detection of a fluorescence spectrophotometer, the linear range is 105-107 CFU / mL, and R2 is equal to 0.9994. The whole detection process is very simple, high in sensitivity and strong in specificity, and can be finished detection within 1.5 h.

An ELISA kit for detecting a Salmonella pullorum antibody is established by screening Salmonella pullorum dominant antigen GroEL through an immunoprecipitation technology, expressing GroEL recombinant protein through utilizing a prokaryotic expression vector, and utilizing an antigen protein. The kit can reduce the response of chicken in the detection process of the Salmonella pullorum antibody, and can improve the detection specificity and the sensitivity.

The invention discloses a CLEIA detection kit and a detection method of salmonella. The kit includes a salmonella antibody, a horseradish peroxidase labeled salmonella antibody and a chemiluminescent solution containing p-lodophenol, luminol and hydrogen peroxide. The corresponding detection method uses the salmonella antibody coated in a 96 well microtiter plate as a capture antibody, the horseradish peroxidase labeled salmonella antibody as an antibody for detection, and luminol-H2O2 with a p-iodophenol reinforcing agent as the chemiluminescent solution, so as to establish a double antibody sandwich chemiluminescent enzyme immunoassay method for detecting content of salmonella. The method provided by the invention has the advantages of high sensitivity, good selectivity, easy operation, fast detection and low cost, can realize large-scale online detection of salmonella, and is suitable for extensive application in the fields of food safety and environmental monitoring, etc.

The invention provides a kit used for detecting swine salmonella antibody. The kit comprises antigen protein; the antigen protein is fljB protein, and the amino acid sequence of the antigen protein isrepresented by SEQ ID NO.3. The fljB protein is excellent in antigenicity, and high in specificity, purity, and yield; in the kit, the antigen protein coating concentration is 20<mu>g / ml, and negative and positive serum dilution ratio is 1:200. The kit can be used for swine salmonella fljB antibody rapid detection of clinical samples, is capable of solving a problem in the prior art that there isno single antigen salmonella infection diagnosis method, reducing failure detection rate, and increasing precision. A sensitive detection method is provided for swine salmonella epidemiological investigation in China, and the kit can be used for early diagnosis of swine salmonella infection.

The invention relates to an ELISA kit for detecting Salmonella antibody, a detection method and application thereof, and belongs to the technical field of microbial immunology. It comprises antigenic protein or antigenic protein solution or a coated plate coated with said antigenic protein solution, enzyme-labeled antibody, color development solution, stop solution, positive serum and negative serum. The kit can quickly, specifically and sensitively detect Salmonella antibodies in serum, and the sensitivity of detecting serum antibodies is 1:1000; the kit is easy to operate, low in cost, easy to observe the reaction results, high in sensitivity and strong in specificity, and is suitable for Salmonella monitoring, epidemiological investigation and detection of clinical samples and other technical fields.

The present disclosure provides anti-Salmonella antibodies or antibody fragments, such as camelid single domain antibodies (VHHs), along with associated nucleic acids, host cells and phages. Methods of reducing the presence of Salmonella in an animal or an animal environment, methods and formulations for treating Salmonella infection, and methods of detecting Salmonella are also described.

The invention discloses a cart for detecting a salmonella antibody in a henhouse. The cart comprises a frame and a box body, wherein the frame is provided with a box body supporting plate and wheels; the box body supporting plate is arranged at the upper part of the frame and is used for supporting the box body; the wheels are arranged below the frame; the box body is a box-shaped component; the top surface of the box body is transparent; a lamp holder is mounted in the internal space of the box body and is used for holding a bulb. An incandescent bulb or an energy-saving lamp can be mounted on the lamp holder according to the seasons, an appropriate environment temperature is provided for a detection material placed on the top surface, a light source is stable, and observation is facilitated.