Method for sensitively, simply and conveniently detecting bacteria
A technology of bacteria and targets, applied in the field of microbiology and chemical biology, can solve the problems of many steps and complicated detection process, and achieve the effect of simple steps, simple detection process and high sensitivity
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[0025] Example 1 Salmonella typhimurium S . typhimurium detection
[0026] Salmonella typhimurium S . typhimurium As an example, the method of the present invention is described in detail.
[0027] 1. Method
[0028] 1. Preparation of immunomagnetic and immunofluorescent spheres
[0029] The preparation of magnetic nanospheres (MNS) and fluorescent nanospheres (FNS) is based on the preparation of fluorescent magnetic bifunctional nanospheres in our laboratory. That is, the ultrasonic swelling method is used to make the oil-soluble magnetic nanoparticles γ-Fe 2 o 3 Alternatively, quantum dots (CdSe / ZnS QDs) were embedded into the hydrophobic cavity of surface carboxylated styrene-acrylamide copolymer nanospheres (Pst-AAm-COOH), resulting in MNS and FNS. The diameter of the nanospheres is about 250 nm. Before attaching the antibody on the surface of the nanosphere, introduce NH by covalent coupling 2 -PEG-COOH (MW 3400) as a bridge (spacer): Take about 5 mg MNS or...
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