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Detection card for detecting Salmonella enteritidis in tableware

A technology of Salmonella enteritidis and Salmonella, applied in measuring devices, immunoassays, instruments, etc., can solve the problems of differences in sensitivity, specificity and stability, repeated development, blanks, etc., and achieve high specificity and sensitivity, preparation method Simple, easy-to-operate effect

Inactive Publication Date: 2017-05-31
BEOSON JIANGSU FOOD SAFETY TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Detection tools, new equipment and detection cards are emerging in an endless stream, especially in the fields of immunology, biochemistry and molecular biology, various new types of rapid detection detection cards have been developed, which can improve the detection speed of Salmonella and simplify sample processing. Complicated steps while reducing the operational requirements of testing personnel, making them dependent on the use of testing cards
However, test cards for testing are widely used in the market, but there is no unified management system and standardized reference standards to evaluate them, resulting in uneven quality of these test cards, manifested in sensitivity, specificity and stability There are some differences, so that the authenticity of the test results between various laboratories / inspectors cannot be guaranteed, and it is inevitable that there will be false detection or missed detection of Salmonella Enteritidis in animal foods, which may not only lead to the failure of my country's import and export in international trade. significant economic losses, and may pose a threat to human health
At present, the management of animal food safety testing and testing cards in my country is basically blank. In order to improve the detection efficiency of Salmonella Enteritidis and at the same time ensure the testing capabilities of laboratories and testing personnel and ensure the validity of testing results, it is necessary to standardize the The use of test card products fills the gap in management. Therefore, it is imperative to develop standard materials that can be used for test cards and daily quality control
[0004] However, at present, there is little research on reference materials in the field of food safety testing at home and abroad, and the research and preparation of microbial reference materials is still in its infancy. The development of standard values ​​in my country began in the 1980s. Basically meet the needs of economic construction, but with the development of science and technology and the continuous emergence of new materials and equipment, the existing standard substance varieties can no longer fully meet the demand, especially in the scope of biological standard substances, the food analysis related to the development of my country There are about 390 kinds of certified reference materials, all of which are biological component analysis reference materials, mainly used in food safety, nutritional evaluation, environmental health evaluation, etc.
Although our country has gone through more than 20 years of research on standard materials, there are still the following problems: few types, many repeated researches, relatively blank research on standard materials related to microorganisms, livestock and poultry, etc. The accuracy of standard materials is not high, and many standard materials cannot provide reference information for uncertainty

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  • Detection card for detecting Salmonella enteritidis in tableware

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Embodiment Construction

[0016] The technical solution of this patent will be further described in detail below in conjunction with specific embodiments.

[0017] see figure 1 , a test card for detecting Salmonella enteritidis in tableware, comprising a sample liquid absorbing part 2, a bottom plate 1, a gold-labeled Salmonella antibody layer 3, a detection reaction part 4 and a water-absorbing part 7, which are placed on the backing of the bottom plate 1 in sequence from left to right A sample liquid absorbing part 2, a gold-labeled Salmonella antibody layer 3, a detection reaction part 4 and a water-absorbing part 7 are attached, and the detection reaction part 4 connected to the gold-labeled Salmonella antibody layer 3 is coated with an anti-Salmonella enteritidis antibody (polyantibody or monoclonal antibody) Anti-) detection line 5 and control line 6 formed by rabbit anti-mouse IgG antibody.

[0018] The preparation method of the gold-labeled Salmonella antibody layer 3 comprises the following s...

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Abstract

The invention discloses a detection card for detecting Salmonella enteritidis in tableware. The detection card comprises a sample liquid absorption part, a baseboard, a gold marked salmonella antibody layer, a detection reaction part and a water absorption part, and the sample liquid absorption part gold marked salmonella antibody layer, the detection reaction part and the water absorption part are sequentially applied to the back lining of the baseboard from the left to the right. The detection card for detecting the residual condition of the Salmonella enteritidis in tableware has the advantages of high sensitivity and sensitivity, simplicity in operation, and no special facilities. The detection card has high specificity and sensitivity, is rapid, fast and convenient to operate in the detection process, and is suitable for being used in clinic examination, epidemiologic investigation and place quarantine. The detection card has the advantages of simple preparation method, good stability and good repeatability.

Description

technical field [0001] The invention relates to a detection test paper, in particular to a detection card for detecting Salmonella enteritidis in tableware. Background technique [0002] Salmonella enteritidis (SE) is a common pathogen that causes food poisoning and is of great significance in public health. Poultry, eggs and meat products are the main media of SE, which seriously affects the development of the breeding industry and human health. , so food that is susceptible to SE contamination should be strictly tested before entering the market. [0003] The safety of animal food has been widely concerned as a social health issue, and the corresponding detection capabilities are constantly improving, and the safety detection technology is being developed in the direction of accuracy, speed and convenience. Detection tools, new equipment and detection cards are emerging in an endless stream, especially in the fields of immunology, biochemistry and molecular biology, vario...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/558G01N33/531
CPCG01N33/56916G01N33/531G01N33/558G01N2469/10
Inventor 周合张根义张进周朱晨杨敏胡彬吴念绮
Owner BEOSON JIANGSU FOOD SAFETY TECH CO LTD
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