Preparation method and application of salmonella inactivated vaccine

Abstract

The invention discloses a preparation method and application of a salmonella pullorum gene mutant strain inactivated vaccine. The preparation method comprises the steps that a lambda-Red system is used for preparing a salmonella pullorum pagC gene deletion mutant strain delta PagC; delta PagC bacterial liquid cultured overnight is taken and inoculated into 50ml of a liquid LB culture medium according to a ratio of 1:100, and shaking culture is conducted at 37 DEG C and 180rpm until an OD value is about 0.8; a formaldehyde solution with the final concentration of 0.4% is added, shaking cultureis continuously conducted for 16 hours, centrifuging is conducted for 10 minutes at the temperature of 4 DEG C and the speed of 5000rpm, and thalli are collected, washed with a sterile PBS buffer solution, and resuspended for three times to obtain a stock solution; the dosage is adjusted, the stock solution is mixed with an adjuvant according to a ratio of 1:1, fully emulsifying is conducted, andthen the inactivated vaccine is inoculated to animals. By preparing the salmonella inactivated vaccine, no adverse reaction is caused after the vaccine strain is inoculated to a mouse, and the mouse can be protected against infection of salmonella pullorum CVCC1800, salmonella enteritidis ATCC19585 and salmonella typhimurium CVCC542. The vaccine can be used in combination with a salmonella antibody indirect ELISA detection kit based on PagC protein, differential diagnosis of salmonella artificial immunity and natural infection is achieved, and good DIVA characteristics are shown.

Description

Technical field [0001] The present invention relates to the technical field of veterinary student products, and is a method for preparation of Salmoni sterilized vaccine and a use of Salmoni. Background technique [0002] Salmoni is a class of important foodborne pathogen microorganisms, which have more than 2,500 kinds of serotypes, most of which have pathogenic, causing animal diarrhea, enterfearia, and poultry, the meaning of livestock farming . Sick and strain animals are the main infection sources of this disease, not only continuous detoxification in populations, but also vertically to the next generation. The traditional method of positive purification according to clinical conditions cannot be eliminated in time to phase out the hidden infection group, and the purification of Salmoni is needed to adopt a plan for vaccine immunity and serological testing, so it is urgent to develop a Diva (Differentiation of Infected) And vaccinated animals vaccine can distinguish between ...

AI Technical Summary

Problems solved by technology

The traditional method of positively purifying groups based on clinical symptoms cannot eliminate recessively infected groups in time. The purification of Salmonella requires a combination of vaccine immunization and serological testing. Therefore, it is urgent to develop a DIVA (Differentiation of Infected and Vaccinated Animals) vaccines, which can distinguish clinical infection from artificial immunity in serological testing

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