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A method for identifying prawns using a specific peptide group

A technology for prawns and species, applied in the biological field, can solve the problems of laborious and expensive, time-consuming, false negatives, etc.

Active Publication Date: 2021-01-12
INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the identification methods of prawn species are mainly based on DNA-based PCR technology and protein electrophoresis technology. Among them, PCR technology is mainly based on DNA detection, and the operation steps are cumbersome, time-consuming, laborious and expensive, and processed shrimp products The DNA is easily damaged, resulting in false negatives; in the process of shrimp processing, it is easy to cause protein instability, which will lead to false negative results in protein electrophoresis or enzyme-linked immunosorbent techniques

Method used

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  • A method for identifying prawns using a specific peptide group
  • A method for identifying prawns using a specific peptide group
  • A method for identifying prawns using a specific peptide group

Examples

Experimental program
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Effect test

Embodiment 1

[0087] Example 1, Sequence Source and Alignment Information of Penaeus monodon Specific Polypeptide

[0088] The above polypeptide SEQ ID NO.1: VAVTDGDADAAVADLH comes from the protein hemocyanin subunit Y, and its Accesion number on NCBI is ABR14694.1. After mass spectrometry analysis, the polypeptide SEQ ID NO.1: VAVTDGDADAAVADLH has an independent response in Penaeus monodon (Penaeus monodon), and the peak shape is good and the intensity is high. In Penaeus ensis, Penaeus vannamei , Penaeus japonicus, Penaeus merguiensis and Fenneropenaeus chinensis showed no response.

[0089] figure 1 is the chromatogram of polypeptide VAVTDGDADAAVADLH in Penaeus monodon, its m / z is 770.4.

Embodiment 2

[0090] Example 2, the sequence source and comparison information of the new prawn (Metapenaeus ensis) specific polypeptide

[0091] The above polypeptide SEQ ID NO.2: AEGLIQEAK comes from protein myosin heavy chain type 2, and its Accesion number on NCBI is BAM65722.1. After mass spectrometry analysis, the peptide SEQ ID NO.2: AEGLIQEAK has an independent response in Penaeus ensis (Metapenaeus ensis), and the peak shape is good and the intensity is high. There was no response in Penaeus japonicus, Penaeus merguiensis and Fenneropenaeus chinensis.

[0092] figure 2 It is the chromatogram of peptide AEGLIQEAK in Penaeus clovenae, its m / z is 479.8.

Embodiment 3

[0093] Example 3, Sequence Source and Alignment Information of Penaeus vannamei Specific Polypeptides

[0094]The above polypeptide SEQ ID NO.3: QADLEEMLNEAR comes from protein myosin heavy chain type 1, and its Accesion number on NCBI is BAM65721.1. After mass spectrometry analysis, the peptide SEQ ID NO.3: QADLEEMLNEAR has an independent response in Penaeus vannamei (Penaeus vannamei), and the peak shape is good and the intensity is high. , Penaeus japonicus, Penaeus merguiensis and Fenneropenaeus chinensis showed no response.

[0095] image 3 is the chromatogram of peptide QADLEEMLNEAR in Penaeus vannamei, its m / z is 709.8.

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Abstract

The invention relates to a method for identifying penaeidae species by utilizing a special peptide segment group. The special peptide segment group is one group of characteristic polypeptides which are used independently or in arbitrary combination to identify the penaeidae species, preferably polypeptides shown as SEQ ID No. 1 to SEQ ID No. 31; specifically, the method can be a mass-spectrometricmethod; the method can be used for identifying penaeus monodon, metapenaeus ensis, penaeus vannamei, penaeus japonicus, penaeus merguiensis and fenneropenaeus chinensis in the penaeidae species. Themethod is not influenced by protein stability and a deep processing technology and is an efficient and accurate method for identifying the penaeidae species.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for identifying the species of prawns (Penaeidae) by using a specific peptide group. Background technique [0002] Prawns (Penaeidae) are rich in protein and amino acids, and are a high-quality protein source. Penaeus monodon, Penaeus ensis, Penaeus vannamei, Penaeus japonicus, Penaeus merguiensis and Fenneropenaeus chinensis are common in my country. Shrimp, among which, Penaeus vannamei, Penaeus vannamei and Penaeus monodon are also known as the three major shrimps with the highest aquaculture production in the world. [0003] The flavor, nutritional value and commercial value of different prawns are different, but due to their phenotypic similarity, it is difficult to distinguish them with the naked eye. In addition, prawns often remove their shells, heads and tails in industrial production, which makes the distinction even more difficult. . With the improvement of livi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/435
CPCG01N30/06G01N30/72
Inventor 薛长湖张晓梅张鸿伟胡玲萍林超赵雪徐杰李明哲
Owner INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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