35 results about "Penaeus chinensis" patented technology

The invention relates to a Fenneropenaeus chinensis antimicrobial protein gene and the recombination expression and the application. It has the amino acid sequence of SEQ ID No.2 and basic group sequence of SEQ ID No.1 in sequence table. It selects the carrier of the antimicrobial protein pCR T7 / NT TOPO TA and host bacterium BL21(DE3)pLysS that could high efficiently express recombination protein. Aimed at the feature of plural disulfide bonds, using the oxidation reduction system made of oxidized form of glutathione and glutathione in separating and purification and protein recombination process, the recombination protein with antimicrobial ability would be gained. The protein has strong inhibition to the growth of gram positivebacteria, and some inhibition to gram negativebacteria.

The invention discloses an aquaculture feed for penaeus chinensis. The aquaculture feed is prepared by main and auxiliary materials. The main materials comprise, by weight, 22-26 parts of fish paste, 23-25 parts of fish meal, 8-12 parts of meat and bone meal, 8-12 parts of blood meal, 10-14 parts of yeast powder, 4-6 parts of sunflower seed cake, 1-3 parts of sugarcane slag powder, 8-12 parts of silkworm chrysalis, 0.8-1.2 parts of fish oil, 6-8 parts of soybean phosphatide, 2-4 parts of cuttlefish paste, 0.2-0.4 part of calcium dihydrogen phosphate, 0.1-0.2 part of green tea powder, and 1-2 parts of black bean powder. The auxiliary materials comprise, by weight, 0.02-0.04 part of insect chitin, 0.01-0.03 part of vitamin premix agent, 0.05-0.07 part of rhizoma polygonati, 0.02-0.04 part of hypericum japonicum, 0.02-0.04 part of medicated leaven, and 0.02-0.04 part of Taibai ginseng. The preparation method comprises the steps of mixing the main materials, granulating, spraying a solution of the auxiliary materials on the particles of the main materials, and drying. Feeding shrimps with the aquaculture feed for penaeus chinensis provided by the invention can promote shrimps to fully absorb nutrition, prevent shrimp diseases, promote shell growth and the shelling of shrimps, improve the yield, and reduce the feed coefficient.

The invention aims to provide a molecular marker relevant to growth of fenneropenaeus chinensis and an application of the molecular marker. The molecular marker is a SNP (Single Nucleotide Polymorphism) locus in a FaMeT gene of the fenneropenaeus chinensis, which is relevant to the growth trait of the fenneropenaeus chinensis. The SNP locus is the 364th site of the FaMeT gene with a nucleotide sequence shown in SEQ ID NO:1, wherein the basic group of the SNP locus is G or T. The SNP locus is obtained by screening the GG-type fenneropenaeus chinensis; the weight and the head cuirass width of the SNP locus are both higher than those of a GT genotype individual. Thus, the GG-type individual can be preferentially selected as a breeding parent according to the SNP locus during the growth breeding selection process of the fenneropenaeus chinensis, thereby improving the efficiency of breeding selection.

The invention discloses a jellyfish breeding method including subjecting jellyfishes to mixed culture with ruditapes philippinarum, sinonovacula constrictas, penaeus chinensis, penaeus monodons and goby; controlling ecological breeding technologies including a stocking system based on breeding species and density, a feeding system based on a match between the species and number and a water quality control system based on a principle that firstly applying fertilizer into water, secondly maintaining fertilizer content and thirdly controlling the water quality. In this way, the jellyfishes and other species are rapid to grow and high in output and economic benefit. By the use of the jellyfish breeding method, the jellyfish output reaches 200-400kg / mu, the outputs of the ruditapes philippinarum and the sinonovacula constrictas reach 200-300kg / mu, and the outputs of the penaeus chinensis and the penaeus monodons reach 10-30kg / mu, and the goby output reaches 20-30kg / mu.

This invention provides a method for simultaneous detecting white spot syndrome virus (WSSV) and taura syndrome virus (TSV) by two-temperature multiplex PCR. The method comprises: (1) designing two pairs of specific primers according to conserved sequences of WSSV and TSV; (2) simultaneously detecting WSSV and TSV by two-temperature multiplex PCR. The method has such advantages as simple operation, rapid detection, high specificity, high sensitivity and low cost. the method can be used for simultaneous detection of WSSV and TSV, Penaeus chinensis health investigation, epidemic control and differential diagnosis.

The invention discloses a method for efficiently establish half-sib families for penaeus chinensis. The method includes steps of 1), strengthening nutrition cultivation for family penaeus; 2), marking the family penaeus individually for breeding; and 3), regulating the three steps with environmental conditions. The method for efficiently establishing the half-sib families for the penaeus chinensis simple to operate can remarkably improve success rate of establishing the half-sib families, and can meet subsequent requirement for large-scale penaeus chinensis breeding.

The invention discloses a penaeus chinensis feed. The penaeus chinensis feed is made from the following materials in parts by weight: 100-200 parts of fish meal, 50-100 parts of crab shell and shrimp shell extracts, 30-50 parts of soyabean protein powder, 30-50 parts of puffing corn powder, 10-20 parts of oyster powder, 10-20 parts of feather meal, 15-25 parts of bone meal, 20-30 parts of tapioca starch, 10-20 parts of pueraria starch, 7-15 parts of red jujube powder, 20-30 parts of cassava distillers' grains, 1-5 parts of yeast powder, 50-100 parts of milk and 10-20 parts of an additive. The feed disclosed by the invention does not contain antibiotics, chemical synthesis medicines, chemical hormones and the like; vegetable protein and animal protein are compounded for use, so that enough nutrition and enough energy are provided for the penaeus chinensis; the additive contains multiple vitamins, trace elements, vegetable oil, fish oil and plant extraction solutions, so that the stress resistance and the organism immune competence of the penaeus chinensis are enhanced, the prevalence rate of the penaeus chinensis is reduced, the quick growth of the penaeus chinensis is promoted, and the quality of the penaeus chinensis is increased.

The invention discloses a female specific primer of Penaeus chinensis and application thereof. The sequence of the female specific primer is as follows: Femal-specific-F: 5'-GTTTCACCTACGCTTCACCC-3', and Femal-specific-R is: 5'-TCCTTAATCCTGCTGCATCCA-3'. The primer is used for carrying out PCR (Polymerase Chain Reaction) amplification on genome DNA (Deoxyribose Nucleic Acid) of Penaeus orientalis to be detected, if a electrophoresis result shows that two bands of 210 bp and 392 bp appear, the Penaeus orientalis are judged to be female Penaeus chinensis, and if only the band of 392 bp appears, the Penaeus orientalis are judged to be male Penaeus chinensis. The invention also utilizes a designed contrast primer to avoid misjudgment of the identification result caused by the problem of the template, the accuracy is improved. The specific primer provided by the invention has strong specificity, and the identification method is simple, rapid and accurate, and is especially suitable for early genetic sex identification of the penaeus chinensis.

The invention provides a method for establishing a technical analysis system of prawn MSAP (methylation sensitive amplification polymorphism). The method includes: firstly, extracting prawn genome DNA (deoxyribonucleic acid), and diluting for standby; secondly, establishing a double digestion system and a ligation system; thirdly, establishing a pre-amplification reaction system and a pre-extension reaction program; fourthly, establishing a selective-extension reaction system and a reaction program; and fifthly, performing polyacrylamide gel electrophoresis and silver stain to obtain a required stripe. The method provides basis for search on methylation and epigenetic adaptive mechanism of Fenneropenaeus chinensis genome DNA and solves the problem that adaptation of Fenneropenaeus chinensis cannot be researched by DNA methylation. The method is applicable to germplasm resources identification, gene expression regulation and genetic marking of Fenneropenaeus chinensis, has the advantages of high polymorphism, low cost and clear methylation sites, and can be used to analyze methylation level of whole genome without knowing DNA sequence in advance.

The invention belongs to the fields of gene products and application and relates to a method for preparing a soluble penaeus chinensis SWD antibacterial peptide through yeast recombinant expression. The method includes the following steps that 1, a swd / PGAPZ alpha-A recombinant expression vector is established; 2, a high-expression penaeus chinensis yeast strain swd / PGAPZ alpha-A / GS115 is obtained; 3, optimal yeast fermentation culture and expression conditions are established; 4, a SWD recombinant protein is purified; 5, activity detection is conducted on the SWD recombinant protein. The method establishes a simple and efficient yeast eukaryotic expression system to simply and efficiently produce and prepare the SWD antibacterial peptide, and the SWD antibacterial peptide produced by means of the method is soluble in water, high in activity and easy to purify and recover, meanwhile has the double functions of antisepsis and protease activity inhibition, has the advantage of industrial production and has wide application prospect in the fields of feed and food additives, daily chemical industry and health and medicines.

A detection technique for the microsatellite marker B683 of Penaeus chinensis: first extract the genomic DNA of Penaeus chinensis; then use the microsatellite sequence containing B683 in the genome library of Penaeus chinensis to design specific primers at both ends of the sequence; and analyze the genome of Penaeus chinensis The DNA was amplified by PCR, and the PCR products were separated by denatured polyacrylamide gel electrophoresis to obtain the genetic polymorphism map of the B683 core sequence region of Penaeus chinensis. The genotype at the B683 locus of each individual of Penaeus chinensis can be obtained by analyzing the bands of the map. The invention is applicable to detection technologies such as genetic markers, pedigree authentication, and genetic map construction of Chinese prawn populations. The method is convenient, fast and accurate, and can visually detect the genotype of each individual of Penaeus chinensis at the B683 locus.

The present invention discloses a Fenneropenaeus chinensis conditioning food and a processing method thereof, wherein the problems of simple process and single taste and nutrition of the traditional shrimp conditioning food preparation process are solved and the shelf life of the product can be prolonged through the present invention. The Fenneropenaeus chinensis conditioning food is characterized in that the Fenneropenaeus chinensis conditioning food contains Fenneropenaeus chinensis, chicken meat, starch, common yam rhizome, monosodium glutamate, edible salt, allium fistulosum-ginger juice, white sugar, edible oil, piper nigrum powder, onion, cooking wine, astaxanthin and vitamin C, and Fenneropenaeus chinensis washing, sterilization, minced meat preparing, mixing seasoning, molding, slurry preparation, slurry coating, packaging sterilization, rapid freezing and other steps are performed to prepare the Fenneropenaeus chinensis conditioning food. The process preparation method of the present invention can further be widely used for production of other shrimp conditioning foods.

The invention belongs to the field of gene product development and application and relates to a production method and application of a penaeus chinensis COMT (Catechol-O-methyltransferase) recombinant protein. The technical scheme is as follows: the production method of the penaeus chinensis COMT recombinant protein comprises the following steps of (1) constructing a comt / pPIC9K recombinant expression vector; (2) obtaining a Pichia pastoris expression strain comt / pPIC9K / KM71 with a large transformant copy number; (3) carrying out fermentation culture and inducible expression on transformants; and (4) purifying COMT. The COMT prepared from eukaryon-Pichia pastoris by using the method has translated modification so as to be more natural in structure and higher in enzymatic activity; the COMT is directly purified by using a yeast liquid, so that the complex protokaryotic thallus recycling and smashing steps are omitted, the production cost is reduced, and the purification efficiency is increased.

The invention relates to the technical field of food processing, in particular to seafood flavored pizza. The seafood flavored pizza comprises the following raw materials by weight of 100-500g of flour, 1-4g of dry yeast, 50-80g of warm water, 100-500g of fresh penaeus chinensis, 300-400g of fresh squids, 30-50g of bacon, 10-30g of fresh peas, 30-50g of chili peppers and 50-300g of Mozzarella cheese. The seafood flavored pizza which is good in color, fragrance and taste is made by a simple method, is simple in technology, is suitable for people to make at home, is rich in nutrient components and is suitable for old and young people.

The invention discloses a molecular marker C2 for identifying the sex of penaeus chinensis and application of the molecular marker C2. The nucleotide sequence of the molecular marker C2 is as shown in SEQ ID No.1, and the molecular marker C2 is a specific molecular marker for female penaeus chinensis. Meanwhile, a pair of amplification primers with nucleotide sequences as shown in SEQ ID No.2 and SEQ ID No.3 are also obtained by utilizing the molecular marker C2. In the invention, the genome DNA of the penaeus chinensis to be detected is used as a template, an amplification primer pair is used for PCR amplification, then a PCR amplification product is detected by electrophoresis, when a 192 bp specific amplification band appears in the amplification product, the penaeus chinensis is judged to be female, and when no amplification product appears, the penaeus chinensis is judged to be male. The molecular marker C2 and the amplification product thereof which are used for identifying the sex of the penaeus chinensis are simple to operate, accurate in result and not easy to misjudge, and are not limited to the development period of the penaeus chinensis.

The invention discloses a processing method of a penaeus chinensis feed. The processing method is characterized by comprising the following aspects of (1) performing cooking: crushing soybean meal andcamellia seed meal, stirring and mixing the crushed soybean meal with the crushed camellia seed meal, fish meal, fish internal organ powder and walnut oil, and performing cooking to obtain a cooked mixture; (2) performing fermentation: adding a mixed bacterium agent 1 and a mixed bacterium agent 2 to the cooked mixture, and performing fermentation; (3) performing mixing: adding dry beer yeast, shrimp shell powder, lotus root starch, red algae powder and soybean phospholipid to the fermented mixture, adding a plant gum solution to the mixture, and performing stirring so as to obtain mixed feedpowder; (4) performing spraying: granulating the mixed feed powder to obtain a granular feed, and spraying a functional agent to the granular feed; and (5) performing film coating: adding hardened beef tallow and casein to a plant extract, performing stirring and mixing to obtain a film coating agent, and performing macerating.

The invention discloses a breeding method of a fast-growing stress-resistant type penaeus chinensis commercial line, and belongs to the technical field of penaeus chinensis breeding. The method comprises the following steps: collecting penaeus chinensis Huanghai No.1 population for pure breeding, selecting excellent female prawn and male prawn parents from the penaeus chinensis Huanghai No.1 pure breeding population as ancestral generations, and breeding a family with high growth performance as a specialized maternal line; collecting penaeus chinensis Huanghai No.3 and Huanghai No.4 populations for pure breeding, selecting male prawn parents with high ammonia nitrogen resistance and high stress resistance from the penaeus chinensis Huanghai No.3 pure breeding populations, selecting female prawn parents with high pH resistance and high stress resistance from the penaeus chinensis Huanghai No.4 pure breeding populations as ancestral generations; breeding a family with high stress resistance as a specialized paternal line by adopting an artificial insemination technology; selecting female prawns from the specialized maternal line, selecting male prawns from the specialized paternal line, carrying out hybridization by adopting the artificial insemination technology, and obtaining the fast-growing stress-resistant type penaeus chinensis commercial line.

The invention relates to a primer set for identifying a Chinese prawn population. The primer set comprises four pairs of primers, wherein sequences of a forward primer and a reverse primer of each pair of primers are SEQ ID NO: 1 and SEQ ID NO: 2, SEQ ID NO: 1 and SEQ ID NO: 3, SEQ ID NO: 4 and SEQ ID NO: 5, and SEQ ID NO: 6 and SEQ ID NO: 7 respectively. According to a primer combination screened by the invention, the source and the place of the Chinese prawn population at a natural sea area can be determined, and the contribution of different spawning sites to the Chinese prawn population is favorably defined. Chinese prawns are widely distributed in the sea area shared by China, Japan and Korea; according to the primer combination screened by the invention, the contribution of different spawning water areas to the condominium water area resource can be determined, and resource place proportion and deserved fishing quota can be defined; and the method has great significance to safeguard of Chinese maritime right and protection of Chinese fishery resources.

The present invention relates to a core sequence, primer and application of a SNP marker related to high pH resistance of Chinese prawns, and belongs to the technical field of shrimp molecular marker-assisted breeding. The core sequence and primer sequence of the SNP marker are respectively shown in SEQ ID NO.1 As shown in -6, the method for using the primers to carry out typing is as follows: extract the individual genomic DNA of Chinese prawns, use the SNP marker typing primers, and adopt the fluorescent quantitative PCR method to carry out SNP marker typing of Chinese prawn individuals to determine the individuality of each individual. genotype. Since the SNP marker FC1516 is closely related to the high-pH tolerance trait of Chinese prawns, and this method is a molecular marker detection method based on the DNA level, the genotype of this locus can be quickly and accurately detected for screening, Eliminate individuals that are intolerant or sensitive to high pH stress, and speed up the process and accuracy of the breeding process and accuracy of high pH stress-resistant traits in Penaeus prawns in China.

The invention provides a preparation method of a laver extract and application of the laver extract in aquaculture. According to the preparation method of the laver extract, laver is extracted at a time through a two-step composite enzymolysis method, and the extract is mainly a mixture composed of polysaccharide, protein and polypeptide. The technological method is simple and easy to implement, the yield of the prepared extract is high, and polysaccharide and protein in porphyra are extracted at a time to the maximum extent. The porphyra extract prepared by the process is added into an aquaculture feed, and when the addition amount is 0.05%-5%, the feed can improve non-specific immunity of fishes, shrimps and crabs and enhance immunity of body cells. Through breeding tests, the immunity,the disease resistance, the growth speed and the survival rate of fishes such as channel catfish and shrimps such as Chinese prawns and crabs are remarkably improved compared with those of a control group. The method disclosed by the invention has the characteristics of simple process, high yield, simplicity in use and various methods, and is suitable for breeding various aquatic animals.

The invention discloses a pond alternate culture method for Chinese prawns and penaeus japonicus. The pond alternate culture method comprises the following steps that S1, preparation treatment is carried out; S2, algae culture fertilizer water and algae phase are balanced; S3, shrimp seeds are selected and put in; S4, culture management is carried out, a Chinese prawn pond and a penaeus japonicus pond are correspondingly managed according to a Chinese prawn culture method and a penaeus japonicus culture method; S5, alternate culture is carried out, after the penaeus japonicus is taken out of the ponds and sold, the Chinese prawn pond and the penaeus japonicus pond are dug through, then half of the Chinese prawns is put in the Chinese prawn pond into the penaeus japonicus pond, and then culture management is carried out on the Chinese prawns in the two ponds according to a Chinese prawn culture method; and S6, the Chinese prawns are taken out of the pond for sale. According to the pond alternate culture method for the Chinese prawns and the penaeus japonicus, the Chinese prawns and the penaeus japonicus are alternately cultured, the utilization rate of the pond is increased, the Chinese prawns and the penaeus japonicus are not affected, the production value per mu reaches 6000 yuan, the benefit per mu reaches 3000 yuan or above, and therefore the purpose of increasing production and income is achieved.

The invention discloses a pond bottom sludge cleaning machine for penaeus chinensis breeding. According to the pond bottom sludge cleaning machine for penaeus chinensis breeding, a sludge cleaning mechanism and a sludge smashing mechanism are arranged at the bottom of a sludge cleaning ship plate, the sludge cleaning mechanism comprises a gear mounting housing, the side wall of the gear mounting housing is hinged to the side wall of the front end of the sludge cleaning ship plate, an underwater motor is mounted on the side wall of the end, close to the sludge cleaning ship plate, of the gear mounting housing, the output end of the underwater motor is sleeved with a driving gear, a first driven gear is meshed with the upper portion of the driving gear, a second driven gear is meshed with the position, located above the sludge cleaning ship plate, of the first driven gear, a sludge cleaning rotating cylinder and a sludge containing box are installed at the position, located on the left side of the underwater motor, of the bottom of the sludge cleaning ship plate through a lifting mechanism respectively, a connecting rotary rod is connected to the rotating end in the middle of the driving gear in a sleeving mode, and first rotary sleeve wheels are connected to the outer walls of the front side and the rear side of the connecting rotary rod. According to the machine, crushing rotating rods and spiral stirring blades are driven to rotate and stir, massive sludge on the front side of the sludge cleaning rotating cylinder is minced, and the sludge can be more conveniently shifted into the sludge containing box through a sludge shifting plate.

The invention discloses a jellyfish breeding method including subjecting jellyfishes to mixed culture with ruditapes philippinarum, sinonovacula constrictas, penaeus chinensis, penaeus monodons and goby; controlling ecological breeding technologies including a stocking system based on breeding species and density, a feeding system based on a match between the species and number and a water quality control system based on a principle that firstly applying fertilizer into water, secondly maintaining fertilizer content and thirdly controlling the water quality. In this way, the jellyfishes and other species are rapid to grow and high in output and economic benefit. By the use of the jellyfish breeding method, the jellyfish output reaches 200-400kg / mu, the outputs of the ruditapes philippinarum and the sinonovacula constrictas reach 200-300kg / mu, and the outputs of the penaeus chinensis and the penaeus monodons reach 10-30kg / mu, and the goby output reaches 20-30kg / mu.

The invention discloses a family breeding method for Penaeus chinensis 'Huanghai No.1' with high ammonia nitrogen resistance and high pH value. The method comprises the following steps of: collecting the Penaeus chinensis 'Huanghai No.1' bred in various areas to construct base groups; then, selecting parent shrimps from different groups, and establishing families according to the complete diallelcross mode to separately grow the larvae and breed; respectively carrying out a high ammonia nitrogen and high pH value stress test on each family at the larvae I stage (P1 stage) and on the day whenthe larvae is 150-day old, and screening the families with high tolerance by combining with the breeding survival rate to carry out breed conservation; in the following year, establishing the families by taking the conservation families as the parents and adopting a nested design, and then breeding in the families; and in the third year, carrying out small-scale demonstrated breeding on the screened families, and determining and estimating the adaptive capacities of the screened families to environment. The method of the invention has the advantages of realizing the polymerization and accumulation of the growth and stress resistance characters of the penaeus chinensis, cultivating new anti-stress strains or varieties after further breeding is performed, providing the adaptability of penaeus chinensis to different breeding environments, and having great significance for the variety replacement of the penaeus chinensis.

The invention relates to a method for cryopreservation of Chinese prawn sperm at ultra-low temperature, and belongs to the technical field of sperm preservation. Specifically, the collected Chinese prawn semen was diluted with ultra-low temperature cryoprotectant solution, then the temperature was lowered to -180°C, and then transferred to liquid nitrogen for storage. In the present invention, the seminal fluid of Penaeus chinensis diluted with ultra-low temperature cryoprotectant solution is cooled down to -180°C and stored in liquid nitrogen. Molecular combination, hydration occurs, and the crystallization process of weakened water increases the viscosity of the solution to reduce the formation of ice crystals. At the same time, it can maintain a certain molar concentration inside and outside the cell, reduce the concentration of electrolyte in the unfrozen solution inside and outside the cell, and make the cell Protects cells from solution damage and ice crystal damage.

The invention relates to a mixed ecological culture method of high-sea swimming crabs and Chinese prawns, and belongs to the technical field of swimming crab breeding. The mixed ecological culture method comprises the following steps of selecting high-sea swimming crab II-stage young crabs with complete appendages, smooth body surfaces and strong healthy vitality and young Chinese prawns with thebody length of 0.9-1.1 cm for mixed ecological breeding; building an outdoor plastic greenhouse pool to temporarily culture the young high-sea swimming crabs, so that the growth period is prolonged, and the marketing specification is improved; and placing scallop cages and arranging shields, so that self-mutilation of the high-sea swimming crabs in the high sea is reduced, and the culture survivalrate is increased. The problems of small marketing specification, low culture survival rate and low comprehensive benefit of the high-sea swimming crabs are solved, the marketing specification of thehigh-sea swimming crabs reaches 100 g or above, the culture survival rate reaches 35% or above, and the comprehensive benefit reaches 9000 yuan / mu.