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A prawn pcab7 protein and wssv envelope protein vp28 interaction blocker polypeptide

A drug and composition technology, applied in the direction of peptide/protein components, peptides, antiviral agents, etc., to achieve the effect of prolonging the survival time

Active Publication Date: 2022-04-12
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

[0005] Although the precise mechanism of WSSV virus entry into the shrimp body is still unknown, related studies have shown that the virus enters host cells through the interaction of the virus envelope protein with the host cell receptors

Method used

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  • A prawn pcab7 protein and wssv envelope protein vp28 interaction blocker polypeptide
  • A prawn pcab7 protein and wssv envelope protein vp28 interaction blocker polypeptide

Examples

Experimental program
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Embodiment 1

[0017] The polypeptide of the present invention is synthesized by bioca.

[0018] Live Chinese prawn is cultured by the applicant experimental base.

[0019] 1 Virus extraction: Take the shrimp tissue of the infected virus to add 5 times volume (V / V) TNE (plus PMSF) homogenate, and the homogenate is 2500 × g 4 ° C for 10 min. Take the supernatant, centrifuge at 10,000 × g at 4 ° C for 30 min. The filter was over 0.45 μm. Take the supernatant 100000 × g from 4 ° C for 1 h. The extracted WSSV particles are suspended in aseptic phosphate buffer (PBS);

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Abstract

The invention provides a blocking polypeptide, which can block the interaction between Chinese prawn PcRab7 protein and WSSV envelope protein VP28, and has anti-WSSV effect. The amino acid sequence of the blocking polypeptide provided by the present invention is TAPLAITAIIAVFI. The polypeptide provided by the invention can block the binding of PcRab7 to VP28, thereby affecting the fusion of the virus envelope and the host cell membrane, so that the virus cannot enter the host cell and realize the anti-WSSV effect. The present invention uses shrimp infection experiments to verify that it can effectively prolong the survival time of shrimps infected with WSSV, can be used as an effective anti-WSSV candidate drug, and provides possible polypeptide drugs for the prevention and treatment of WSSV in the future.

Description

Technical field [0001] The present invention relates to the field of biomedical technology, in particular to block China's penetratin VP28 interaction in shrimp protein PCRAB7 and white spot syndrome, inhibiting white spot syndrome viruses infected with Chinese shrimp. Background technique [0002] The shrimp white spot syndrome is a viral disease caused by White Spot Syndrome Virus (WSSV), which is harmful to the global shrimp farm. The viral host is wide, the toxicity is strong. It has 90% -100% lethality rate within 3 to 7 days after the shrimp, which is seriously harmful to the shrimp farm. Therefore, it is important to screen for efficient antiviral drugs. [0003] The main countermeasures and prevention measures of WSSV prevention and treatment are currently prevented to prevent control of WSSV diseases in a certain extent, and there is still no effective prevention of drugs. Including WSSV recombinant protein subunit vaccines, RNAi technology has good results in the labora...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08A61K38/10A61P31/20
CPCC07K7/08A61P31/20A61K38/00
Inventor 纪晓峰郑媛盛军
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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