Molecular marker relevant to growth of fenneropenaeus chinensis and application of molecular marker

A technology for growth traits of Chinese prawns, applied in the field of molecular marker genetics and breeding, can solve the problem of lack of markers related to growth traits

Inactive Publication Date: 2014-04-16
QINGDAO AGRI UNIV
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  • Abstract
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Problems solved by technology

However, there is still a lack of reports on markers related to growth traits in Penaeus chinensis

Method used

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  • Molecular marker relevant to growth of fenneropenaeus chinensis and application of molecular marker
  • Molecular marker relevant to growth of fenneropenaeus chinensis and application of molecular marker
  • Molecular marker relevant to growth of fenneropenaeus chinensis and application of molecular marker

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Embodiment Construction

[0022] The method of the present invention will be further described below in conjunction with examples. For the experimental methods that do not indicate specific conditions in the following examples, generally, conventional conditions can be used, such as the conditions described in the "Molecular Cloning Experiment Guide" written by J. Sambrook (Sambrook), or according to the manufacturer's suggestion conditional run. Those skilled in the art can better understand and master the present invention with the help of the examples.

[0023] 1. PCR amplification and polymorphism detection of cDNA sequence of FaMeT gene in Penaeus prawn

[0024] 1. PCR amplification of FaMeT gene DNA sequence of Chinese prawn

[0025] Using the sequence of the FaMeT gene mRNA (GenBank Accession No.: JQ315119) in NCBI, the specific amplification primers P1 and P2 were designed. Using the genomic DNA of 30 Penaeus chinensis as templates, Taq DNA polymerase, buffer environment, Mg 2+ In the presen...

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Abstract

The invention aims to provide a molecular marker relevant to growth of fenneropenaeus chinensis and an application of the molecular marker. The molecular marker is a SNP (Single Nucleotide Polymorphism) locus in a FaMeT gene of the fenneropenaeus chinensis, which is relevant to the growth trait of the fenneropenaeus chinensis. The SNP locus is the 364th site of the FaMeT gene with a nucleotide sequence shown in SEQ ID NO:1, wherein the basic group of the SNP locus is G or T. The SNP locus is obtained by screening the GG-type fenneropenaeus chinensis; the weight and the head cuirass width of the SNP locus are both higher than those of a GT genotype individual. Thus, the GG-type individual can be preferentially selected as a breeding parent according to the SNP locus during the growth breeding selection process of the fenneropenaeus chinensis, thereby improving the efficiency of breeding selection.

Description

technical field [0001] The invention belongs to the technical field of molecular marker genetic breeding, and specifically relates to a molecular marker related to the growth of Chinese prawns and its application. Background technique [0002] Chinese prawn (Fenneropenaeu chinensis) is the main economic shrimp species in the Yellow Sea and the Bohai Sea. The breeding areas are mainly concentrated in the coast of the Yellow Sea and the Bohai Sea in the north of my country. In 1990, it accounted for 70% of my country's shrimp farming output. However, after 1993, due to the influence of germplasm, diseases and environmental factors, the aquaculture production dropped sharply, and the current production only accounts for about 10% of my country's prawn production. Cultivating fine varieties with fast growth, good economic traits and strong stress resistance has become the basis for ensuring the sustainable development of China's shrimp farming industry. [0003] Breeding is cu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 李朝霞张艳王金叶董晓煜王春德方华华
Owner QINGDAO AGRI UNIV
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