Molecular marker relevant to growth of fenneropenaeus chinensis and application of molecular marker
A technology for growth traits of Chinese prawns, applied in the field of molecular marker genetics and breeding, can solve the problem of lack of markers related to growth traits
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[0022] The method of the present invention will be further described below in conjunction with examples. For the experimental methods that do not indicate specific conditions in the following examples, generally, conventional conditions can be used, such as the conditions described in the "Molecular Cloning Experiment Guide" written by J. Sambrook (Sambrook), or according to the manufacturer's suggestion conditional run. Those skilled in the art can better understand and master the present invention with the help of the examples.
[0023] 1. PCR amplification and polymorphism detection of cDNA sequence of FaMeT gene in Penaeus prawn
[0024] 1. PCR amplification of FaMeT gene DNA sequence of Chinese prawn
[0025] Using the sequence of the FaMeT gene mRNA (GenBank Accession No.: JQ315119) in NCBI, the specific amplification primers P1 and P2 were designed. Using the genomic DNA of 30 Penaeus chinensis as templates, Taq DNA polymerase, buffer environment, Mg 2+ In the presen...
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