Method for preparing soluble penaeus chinensis SWD antibacterial peptide through yeast recombinant expression

Abstract

The invention belongs to the fields of gene products and application and relates to a method for preparing a soluble penaeus chinensis SWD antibacterial peptide through yeast recombinant expression. The method includes the following steps that 1, a swd / PGAPZ alpha-A recombinant expression vector is established; 2, a high-expression penaeus chinensis yeast strain swd / PGAPZ alpha-A / GS115 is obtained; 3, optimal yeast fermentation culture and expression conditions are established; 4, a SWD recombinant protein is purified; 5, activity detection is conducted on the SWD recombinant protein. The method establishes a simple and efficient yeast eukaryotic expression system to simply and efficiently produce and prepare the SWD antibacterial peptide, and the SWD antibacterial peptide produced by means of the method is soluble in water, high in activity and easy to purify and recover, meanwhile has the double functions of antisepsis and protease activity inhibition, has the advantage of industrial production and has wide application prospect in the fields of feed and food additives, daily chemical industry and health and medicines.

Description

technical field [0001] The invention relates to a method for recombinantly expressing soluble Chinese prawn SWD antibacterial peptide by yeast, which belongs to the technical field of genetic engineering. Background technique [0002] With the widespread use or even abuse of antibiotics, the problems of antibiotic residues and bacterial resistance have attracted more and more attention from all over the world. It has become a worldwide research topic to develop new antibacterial drugs to replace traditional antibiotics as soon as possible. [0003] Antimicrobial peptides are a class of small molecular polypeptides with antibacterial effects discovered in insects in the 1970s. They exist in most organisms and play a very important role in invertebrates' resistance to the invasion of bacteria, viruses, fungi and other pathogenic bacteria. role. Antimicrobial peptides can be used not only in pharmaceuticals but also in food, animal husbandry, aquaculture, daily chemical indust...

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Problems solved by technology

[0006] However, the SWD recombinant protein produced by prokaryotic expression exists in the form of insoluble inclusion bodies (see the paper JiaYP, SunYD, WangZH, WangQ, WangXW, ZhaoXF, WangJX.Asinglewheyacidicproteindomain(SWD)-containingpeptidefromfleshyprawnwithantimicrobialandproteinaseinhibitoryactivities. ), to use it must go through complex denaturation and renaturation steps, which not only reduces the yield, but also increases the difficulty of industrial production

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