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Preparation method of laver extract and application of laver extract in aquaculture

A technology of extract and seaweed, applied in the field of preparation of seaweed extract, to achieve the effects of reducing usage, facilitating large-scale preparation, and improving immunity and survival rate

Pending Publication Date: 2020-11-13
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, studies have shown that microbial polysaccharides, chitin, animal and plant extracts, etc. have varying degrees of immune enhancement effects on aquatic animals, but considering the preparation cost and technical difficulty, the application of these immune enhancers still needs to be further improved

Method used

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  • Preparation method of laver extract and application of laver extract in aquaculture
  • Preparation method of laver extract and application of laver extract in aquaculture
  • Preparation method of laver extract and application of laver extract in aquaculture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Take 100g of crushed seaweed, add 30 times of water, add 1.5g cellulase, 1g pectinase, adjust pH to 5.5, 50°C, react for 2h, add 1.5g trypsin, adjust pH to 8.0, 37°C, 1h; then add 1.5 g alkaline protease, pH 8.0, 50°C, 3h; heat to 95°C to inactivate the enzyme for 15min, centrifuge at 6000rpm for 30min, concentrate the retentate under reduced pressure at 50°C, desalt by nanofiltration, and freeze-dry to obtain laver extract P1.

Embodiment 2

[0024] Embodiment 2, the preparation (P2) of laver extract

[0025] Take 100g of crushed laver, add 40 times of water, add 3.0g cellulase, 3.0g pectinase, adjust pH to 5.0, 55°C, react for 3h, add 3.0g trypsin, adjust pH to 9.0, 40°C, 3h; then Add 3.0 g of alkaline protease, pH 9.0, 55°C, 4h; heat to 90°C to inactivate the enzyme for 20 minutes, centrifuge at 7000rpm for 20 minutes, concentrate the retentate under reduced pressure at 55°C, desalt by nanofiltration, and freeze-dry to obtain laver extract P2.

Embodiment 3

[0026] Embodiment 3, the preparation (P3) of laver extract

[0027] Take 100g of crushed seaweed, add 20 times of water, add 1.0g cellulase, 1.0g pectinase, adjust pH to 5.5, 50°C, react for 2h, add 1.0g trypsin, adjust pH to 9.0, 35°C, 2h; then add 1.5g alkaline protease, pH 7.8, 45°C, 2h; heat to 100°C to inactivate the enzyme for 10min, centrifuge at 7500rpm for 30min, concentrate the retentate under reduced pressure at 50°C, desalt by nanofiltration, and freeze-dry to obtain laver extract P3.

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Abstract

The invention provides a preparation method of a laver extract and application of the laver extract in aquaculture. According to the preparation method of the laver extract, laver is extracted at a time through a two-step composite enzymolysis method, and the extract is mainly a mixture composed of polysaccharide, protein and polypeptide. The technological method is simple and easy to implement, the yield of the prepared extract is high, and polysaccharide and protein in porphyra are extracted at a time to the maximum extent. The porphyra extract prepared by the process is added into an aquaculture feed, and when the addition amount is 0.05%-5%, the feed can improve non-specific immunity of fishes, shrimps and crabs and enhance immunity of body cells. Through breeding tests, the immunity,the disease resistance, the growth speed and the survival rate of fishes such as channel catfish and shrimps such as Chinese prawns and crabs are remarkably improved compared with those of a control group. The method disclosed by the invention has the characteristics of simple process, high yield, simplicity in use and various methods, and is suitable for breeding various aquatic animals.

Description

technical field [0001] The invention relates to the field of aquaculture, in particular to a preparation method of laver extract and its application in aquaculture. Background technique [0002] Aquaculture is developing rapidly in my country, ranking among the best in the world both in terms of output and scale. However, at the same time, the problems restricting aquaculture production have become increasingly prominent, among which the problems of aquatic animal diseases caused by environmental pollution and high-density aquaculture are imminent. Antibiotics and other chemical drugs are still common methods for fish disease prevention and control, but the extensive use of antibiotics will lead to the emergence of drug-resistant strains and the decline of the immunity of aquatic organisms. On the other hand, the accumulation of fishery antibiotics in aquatic products will also directly affect the human body. healthy. Therefore, the development of safe, non-toxic and green...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/14A23K10/30A23K20/142A23K20/147A23K20/163A23K40/30A23K50/80A01K61/59A01K61/50A01K61/10A01K61/00
CPCA23K10/30A23K10/14A23K50/80A23K40/30A23K20/163A23K20/147A23K20/142A01K61/00A01K61/10A01K61/50A01K61/59Y02A40/81
Inventor 王晶王晓晴张全斌耿丽华岳洋吴宁
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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