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Chinese prawn B683 microsatellite label detecting technique

A technology of microsatellite markers and Chinese prawns, which is applied to the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of specific genetic markers of shrimp microsatellite polymorphism maps that have not yet been seen, and achieve a simple and fast method Effect

Inactive Publication Date: 2005-12-28
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

At present, there are no reports on the construction of Chinese shrimp microsatellite polymorphism map, specific genetic markers and pedigree authentication.

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  • Chinese prawn B683 microsatellite label detecting technique

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[0015] The following examples describe in detail the present invention's method for molecular genetic markers of the Chinese prawn B683 microsatellite core sequence DNA. First, extract the genomic DNA of Penaeus chinensis and dilute it for later use; then use the microsatellite core sequence containing B683 in the genome library of Penaeus chinensis to design specific primers at both ends of the sequence; The genomic DNA of the individual is amplified by PCR, and the PCR product is detected by polyacrylamide gel electrophoresis; the bands appearing in the product are analyzed to determine the genotype of each individual, so as to obtain the genetic variation of the Chinese prawn in the B683 core sequence region polymorphism map.

[0016] 1. Extraction of Chinese prawn genomic DNA: Take 100 mg of prawn muscle tissue, cut it into pieces, put it into a 1.5ml centrifuge tube, add 500 μl of pH 8.0 TE solution (10mmol / L Tris-CI, 10mmol / LEDTA), and use a grinding rod grind. Add 25 ...

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Abstract

A detection technique for the microsatellite marker B683 of Penaeus chinensis: first extract the genomic DNA of Penaeus chinensis; then use the microsatellite sequence containing B683 in the genome library of Penaeus chinensis to design specific primers at both ends of the sequence; and analyze the genome of Penaeus chinensis The DNA was amplified by PCR, and the PCR products were separated by denatured polyacrylamide gel electrophoresis to obtain the genetic polymorphism map of the B683 core sequence region of Penaeus chinensis. The genotype at the B683 locus of each individual of Penaeus chinensis can be obtained by analyzing the bands of the map. The invention is applicable to detection technologies such as genetic markers, pedigree authentication, and genetic map construction of Chinese prawn populations. The method is convenient, fast and accurate, and can visually detect the genotype of each individual of Penaeus chinensis at the B683 locus.

Description

Technical field [0001] The invention belongs to the Chinese prawn DNA molecular genetic marker technology, and is a technical method for detecting the genetic polymorphism of the Chinese prawn at the B683 site. Background technique [0002] Before the present invention was made, there were similar research reports both at home and abroad, "Quick Screening of Recombinant Positive Clones Containing Microsatellites in Chinese Prawns by PCR Method" and "Chinese Shrimp Microsatellites" published by Xu Peng et al. Screening of DNA" and other papers have reported the construction of a partial genome library of Chinese prawns and the screening of microsatellite sequences, and evaluated the core sequences of microsatellites according to the standards proposed by Weber (1990), but his research Results were limited to the acquisition of microsatellite sequences for research and no further application development. In 1999, the French Institute of Marine Development (IFREMER) found 10 m...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 孔杰刘萍孟宪红张庆文王伟继费日伟王清印
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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