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Fast marine green algae cultivating process

A marine green algae, rapid technology, applied in the fields of botany equipment and methods, cultivation, plant cultivation, etc., can solve the problems of low chlorophyll content in algal cells, large changes in pH of algae liquid, long growth cycle, etc., and achieve the content of intracellular substances. Rich, fast-growing, simple-to-form effect

Inactive Publication Date: 2007-03-21
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

[0004] At present, marine green algae adopts traditional culture methods, which have a long growth cycle, large changes in the pH of the algae liquid during the culture period, and low chlorophyll content per unit volume of algae cells.

Method used

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Embodiment 1

[0025] The present invention is further illustrated by taking the light culture of Platymonas subcordiformis as an example.

[0026] 1) Preparation of algae cell culture medium: 2 liters of natural seawater filtered through ten layers of gauze was filled in a culture bottle with a volume of 3 liters, and trace elements (mg / l) were added: Tris 1210, CH 3 COONa 1440, NH 4 Cl 375, MgSO 4 ·7H 2 O 129.7, CaCl 2 40.21, Na-EDTA 0.125, ZnSO 4 ·7H 2 O0.5475, FeSO 4 ·7H 2 O 0.349, MnSO 4 ·H 2 O 0.077, CuSO 4 ·5H 2 O 0.0196, Co(NO 3 )·6H 2 O 0.0124, Na 2 B 4 o 7 10H 2 O 0.00885, Nitrilotriacetic acid 10, (NH 4 ) 6 MoO 7 o 24 4H 2 O 0.00925; adjust the pH of the medium to 8.00, and sterilize at 115°C for 20 minutes;

[0027] The Chinese names of the drugs used are: Tris: Tris (hydroxymethyl) aminomethane; CH 3 COONa: sodium acetate; NH 4 Cl: ammonium chloride; MgSO 4 ·7H 2 O: magnesium sulfate heptahydrate; CaCl 2 : Calcium chloride; Na-EDTA: Disodium ethylenediam...

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Abstract

The present invention relates to culture of marine green algae, and is especially the process of fast cultivating marine green algae. The process includes sterilizing and cooling natural sea water and adding trace nutritious elements to form the culture medium; inoculating marine green algae in the density of (5.0-6.0)x10 cells / ml; and lighting culturing in the liquid surface illumination intensity of 3500-4000 Lx and lighting / no-lighting time ratio of 12-14 to 12-10 for 6-8 days to reach algae density of (2.0-2.5)x10 cells / ml. The process has slow change of pH in the algae liquid, high chlorophyll content each algae cell unit and large algae cell. The present invention uses trace elements in culturing marine green algae, and has fast algae cell growing rate and high chlorophyll content each algae cell unit.

Description

technical field [0001] The invention relates to the cultivation of marine green algae, in particular to a method for adding micronutrient elements to regulate the rapid growth of marine green algae. Background technique [0002] Marine Green Algae is a low-level plant that grows in the ocean, and is one of the marine algal cells widely used by humans. Marine green algae are generally rich in nutrients. In addition to being widely used as food industry raw materials, fertilizers and feedstuffs, marine green algae has attracted more and more attention for its health care and medicinal value. It is rich in vitamins and amino acids. , minerals and trace elements, while the content of lipids and digestible and absorbable sugars is low, so the use of seaweed to develop low-calorie and high-nutrition health food has become a current hot topic. Many marine green algae are also rich in bioactive substances and medicinal ingredients, and have important development potential as a new ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G33/00
CPCY02A40/80
Inventor 张卫冉春秋虞星炬金美芳陈兆安
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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