36results about How to "Stable osmotic pressure" patented technology

Technology for bulk preserving spermatozoa of Chinese sturgeon through freezing at ultralow temperature comprises collecting and detecting fresh spermatozoa, diluting, balancing, adding antifreeze, subpackaging, program cooling, and preserving in liquid nitrogen container of -196deg.C. Each bulk can freeze 100 mL fresh spermatozoa. when the first bulk spermatozoa is in the procedure cooling step, after 5 minute, starting diluting the second bulk spermatozoa, and repeating above steps until all spermatozoa is preserved 8-12 h before its effective time. The diluent is Tris-HCl 20-30 millimole, sucrose 30-60 mol, potassium chloride 2-10 millimole, and distilled water 1000mL, and regulating pH to 8-8.5 with HCl. The antifreeze is methanol. The invention can efficiently carry out bulk preservation of Chinese sturgeon spermatozoa through freezing at ultralow temperature and long term ultralow temperature freezing preservation, and provide enough spermatozoa resource for artificial propagation of Chinese sturgeon.

The invention discloses a compound premix capable of enhancing the content of proteins in muscles of a grass carp. The compound premix consists of 10000g of the raw materials in weight mixing ratio: 1000g of compound vitamins, 5000g of compound mineral elements, 1000g of Beta-dextran premix agents, 500 to 1000g of taurine and unite bran. The addition of the compound premix of the invention in theformula feed for grass carp takes a weight proportion of 1 percent to ensure that the condition factor of the cultivated grass carp is larger than 2, the organ body weight proportion is smaller than the weight ratio, 11percent, and the content of the proteins in the muscles of the fish body is larger than 22 percent. The invention has the advantages that the grass carp cultivated using the compound feed of the premix has enhanced the edible part and the better meat quality. Saving the resources, the compound premix of the invention is beneficial to the environmental protection and is more beneficial to the increasing demand by the national citizens for the quality and safety of the aquatic foods.

The invention discloses a device for evaluating performance of a forward osmosis membrane. The device comprises a raw material liquid tank, a feed liquid pool, a forward osmosis membrane component, a draw solution pool, a high-concentration salt pond and a delivery tank, wherein a check valve and an overflow pipe are arranged on an overflow tank of the pool; an aeration pipe is arranged at the bottom; the forward osmosis membrane component comprises a shell; a forward osmosis membrane is arranged inside the shell, and divides the shell into upper and lower lattices, which respectively are a draw solution chamber and a feed liquid chamber; the draw solution chamber is put into a magnetic stirrer; a water inlet / outlet of the draw solution pool is connected with the draw solution chamber of the forward osmosis membrane component; a probe of a conductivity controller is put into the pool; the water outlet of the high-concentration salt pond is connected with the water inlet of the draw solution pool through a pipeline with a strong brine pump; opening and closing of the strong brine pump are controlled by a conductivity meter; the delivery tank is put on an electric scale. The device disclosed by the invention is small in volume, simple and easy to operate, stable in test result, small in required area of the forward osmosis membrane, wide in application range, and applicable to test of the forward osmosis membranes with kinds of sources.

The invention relates to the medical field, particularly to a composition and its use, an umbilical cord preservation preparation and a preparation method thereof. According to the invention, physiological saline is taken as the basic ingredient of umbilical cord preservation liquid, umbilical cord blood plasma is used as the nutritional ingredient source, as a natural substance, umbilical cord blood plasma not only provides multiple nutritional ingredients, but also can maximumly maintain the umbilical cord vitality in order to maintain a microenvironment similar to that in vivo. Lactobionic acid, hydroxyethyl starch and other macromolecular substances are added to avoid swelling death of the umbilical cord in the preservation process. The preservation liquid can be used for transportation and preservation of the preparation at low temperature. Thus, adequate nutrients are supplied to in vitro umbilical cord, and also the seed cell vitality, the original cell morphology and biological characteristics are well maintained.

The invention provides a kind of avelamycin high-yield bacterial strain breeding method, adopts ARTP and three times 137 Csy mutagenesis mutated Streptomyces and used 2 protoplast fusions for gene recombination. The beneficial effect is: after four rounds of mutagenesis and two rounds of protoplast fusion, the fusion high-yield strain G2-31 was obtained, and its avelamycin titer reached 2842.57 mg / L, which was 4 times that of the original strain 11-10. Passage test, strain state is stable, showing high potential for industrial application.

The invention discloses a serum-free cryopreservation solution for immune cells, which is characterized in that it comprises a basal medium and a cell cryopreservation protective agent, and the cell cryopreservation protective agent is composed of the following raw materials: polyethylene glycol, nonylphenol poly Oxyethylene ether-10, acacia polysaccharide, sodium carboxymethylcellulose, polyglyceryl fatty acid ester, bamboo leaf flavone. In the present invention, polyethylene glycol and nonylphenol polyoxyethylene ether-10 are used to replace DMSO to change the permeability of the cell membrane and at the same time protect the cell membrane, so that the free water in the cell can be excluded from the cell and reduce the risk of cryopreservation. The formation of ice crystals in the cells during the process damages the cells. Acacia polysaccharides, sodium carboxymethylcellulose, and polyglycerol fatty acid esters are added as osmotic pressure stabilizers to maintain a stable osmotic pressure in the extracellular environment. Added with bamboo leaf flavonoids, it helps cells recover quickly after recovery. The invention also provides a cryopreservation method for immune cells, which is simple and convenient, and meets the demands for immune cells in scientific research, medical treatment and other fields.

The invention provides mesenchymal stem cells combined with traditional Chinese medicines for activating amplification, and a preparation method of the mesenchymal stem cells comprises the following steps: (1) pretreatment: digesting tissue pieces with pancreatin and IV collagenase, separating primary mesenchymal stem cells; (2) culture: adding the cells obtained in the step (1) to a low sugar DMEM culture medium containing components of 50-80mg / mL hemianthus micranthemoides extract, 50-80mg / mL Chinese gall extract, 1.5-3mmol / mL L-glutamine and 1.5-3ng / mL bFGF, culturing in a wet incubator of 37 DEG C with 5% CO2, changing the culture medium once every 3-4 days; and (3) collection of cells and subculture. Shown by in vitro tests, the traditional Chinese medicines activated mesenchymal stem cells can effectively inhibit reproduction of HBV and promote repair of damaged hepatic cells.

The invention discloses a preparation method of round leaf zannichelliaceae cymodocea rotundta protoplast, and belongs to the field of plant cell engineering. The preparation method comprises the stepS1 of taking spires of round leaf zannichelliaceae cymodocea rotundta, cleaning the spires with sterile seawater, and then performing temporary culture to obtain a raw material 1; the step S2 of immersing the raw material 1 obtained in the step S1 into a pretreatment solution to obtain a raw material 2; the step S3 of taking out the raw material 2 obtained in the step S2, removing the moisture, cutting the raw material into pieces and adding an enzyme solution for enzymatic hydrolysis to obtain an enzymatic hydrolysis tissue; the step S4 of filtering the enzymatic hydrolysis tissue obtained in the step S3 to obtain a filtrate; the step S5 of centrifuging the filtrate obtained in the step S4, abandoning a supernatant, conducting suspension and precipitation with a W5 solution, centrifugingand abandoning the supernatant to obtain a precipitate; the step S6 of suspending the precipitate obtained in the step S5 in an MMG solution to obtain the round leaf zannichelliaceae cymodocea rotundta protoplast. The round leaf zannichelliaceae cymodocea rotundta protoplast obtained by the method has high yield and high survival rate.