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Preparation method of thalassia hemperichii protoplast

A technology of protoplasts and raw materials, which is applied in the field of preparation of Thalias protoplasts, can solve problems such as the absence of Thalesia, and achieve the effects of improved preparation rate, good integrity, and high yield

Active Publication Date: 2019-07-26
HAINAN ACADEMY OF OCEAN & FISHERIES SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Aiming at the problem that there is no preparation method of Thalias protoplast in the prior art, the present invention provides a preparation method of Thaleis protoplast

Method used

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  • Preparation method of thalassia hemperichii protoplast
  • Preparation method of thalassia hemperichii protoplast

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preparation example Construction

[0038] 3.1 Preparation of protoplasts

[0039] Take 2g of Thaleisca young leaves as an example, the specific steps are as follows:

[0040] S1: take the young leaves of Thalassia spp., wash them with sterile seawater, and raise them temporarily to obtain raw material 1;

[0041] S2: Soak the raw material 1 obtained in S1 in the pretreatment solution to obtain raw material 2;

[0042] S3: Take out the raw material 2 obtained in S2, after absorbing the water, cut the raw material 2 into 0.2cm 2 Add 10mL enzyme solution for enzymolysis to obtain enzymolysis tissue;

[0043] S4: Filter the enzymolyzed tissue obtained in S3 with a 200-mesh sieve to obtain a filtrate;

[0044] S5: centrifuge the filtrate obtained in S4 at 800rmp for 10min, discard the supernatant, suspend the precipitate with the resuspension solution, centrifuge, discard the supernatant, and obtain the precipitate;

[0045] S6: suspending the precipitate obtained in S5 with a resuspension solution to obtain Thale...

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Abstract

The invention discloses a preparation method of a thalassia hemperichii protoplast and belongs to the field of plant cell engineering. The preparation method comprises the following steps: S1, takingyoung leaves of thalassia hemperichii, washing them with sterile seawater, and temporarily culturing them to obtain a raw material 1; S2, putting the raw material 1 obtained in the step S1 into a pretreatment liquid for soaking to obtain a raw material 2; S3, taking out the raw material 2 obtained in the step S2, sucking dry water, cutting them into blocks, adding enzyme liquid for enzymolysis, and obtaining enzymolysis tissues; S4, filtering the enzymolysis tissue obtained in the step S3 to obtain filtrate; S5, centrifuging the filtrate obtained in the step S4, discarding the supernatant, suspending the precipitate with a resuspension solution, centrifuging, and discarding the supernatant to obtain a precipitate; S6, suspending the precipitate obtained in S5 with a resuspension solution to obtain the thalassia hemperichii protoplast. The preparation method of the thalassia hemperichii protoplast is established for the first time, and the thalassia hemperichii protoplast obtained by the method is high in yield and high in survival rate.

Description

technical field [0001] The invention belongs to the field of plant cell engineering, and in particular relates to a preparation method of the protoplast of the algae. Background technique [0002] Seagrass is a large monocotyledonous submerged plant that lives in shallow seas in tropical and temperate seas, and belongs to the order Marzophyta. They have the general characteristics of higher plants and are able to complete their life cycle in water. The primary productivity of seagrass is very high, and it is an important component of the shallow water ecosystem - seagrass field. The seagrass field resources are unique, and its ecological and economic value is mainly manifested in: (1) Seagrass can accelerate the settlement of suspended solids in seawater, absorb nutrients and heavy metals in seawater, thereby improving water quality; (2) Seaweed can slow down water flow, thereby Increase the settlement of suspended solids in the water to achieve the effect of stabilizing t...

Claims

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Application Information

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IPC IPC(8): C12N5/04
CPCC12N5/04
Inventor 吴钟解
Owner HAINAN ACADEMY OF OCEAN & FISHERIES SCI
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