A kind of preparation method of simulated cellulase

A cellulase, cellulose technology, applied in chemical instruments and methods, organic compound/hydride/coordination complex catalysts, physical/chemical process catalysts, etc., to achieve high-value conversion, good degradation effect, and preparation process simple effect

Inactive Publication Date: 2016-08-24
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this experimental result is not directly for the catalytic hydrolysis of cellulose

Method used

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  • A kind of preparation method of simulated cellulase
  • A kind of preparation method of simulated cellulase
  • A kind of preparation method of simulated cellulase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Preparation of Morusyl Mimetic Enzymes.

[0032] (1) Take the mulberry branch and cut off the mulberry branch bark with a knife, cut off the mulberry branch bark, wash away the sediment with tap water, dry at 105° C. for 10 h, pulverize, and pass through a 200-mesh sieve to obtain the mulberry branch powder;

[0033] (2) Get 100g mulberry branch powder, add 1000g mass concentration and be the ionic liquid [EMIM][DEP] of 50% (w / v%), process 3h under the condition of 80 ℃, the treatment power of microwave treatment is 900w, treatment time 75s, add 1L of water to the system to precipitate the mulberry branch powder, wash with water 2 to 3 times to remove the ionic liquid (the aqueous solution of the ionic liquid can be distilled to remove water to a certain concentration and can be reused), and place it in a 70°C blast drying oven until dry to obtain the initial sample;

[0034] (3) Put the initial sample obtained in step (2) into a crucible, place it in a tube ...

Embodiment 2

[0037] Example 2 Preparation of Morusyl Mimetic Enzyme.

[0038] (1) Take the mulberry branch and cut off the mulberry branch bark with a knife, cut off the mulberry branch bark, wash away the sediment with tap water, dry at 100°C for 10 hours, pulverize, pass through a 200-mesh sieve, and obtain the mulberry branch powder;

[0039] (2) Get 100g mulberry branch powder, add 1000g mass concentration and be the ionic liquid [EMIM]Ac of 60% (w / v%), process 2h under 90 ℃ of conditions, the processing power of microwave processing is 800w, and processing time 70s, Add 1L of water to the system to precipitate the mulberry branch powder, wash with water 2 to 3 times to remove the ionic liquid (the aqueous solution of the ionic liquid can be distilled to remove water to a certain concentration and can be reused), and place it in a blast drying oven at 75°C until dry , get the initial sample;

[0040] (3) Put the initial sample obtained in step (2) into a crucible, place it in a tube f...

Embodiment 3

[0043] Example 3 Preparation of Morusyl Mimetic Enzymes.

[0044] (1) Take the mulberry branch and cut off the mulberry branch bark with a knife, cut off the mulberry branch bark, wash away the sediment with tap water, dry at 100°C for 10 hours, pulverize, pass through a 200-mesh sieve, and obtain the mulberry branch powder;

[0045] (2) Get 100g mulberry branch powder, add 1000g mass concentration and be the ionic liquid [MMIM][DEP] of 70% (w / v%), process 1.5h under the condition of 1000 ℃, the treatment power of microwave treatment is 850w, treat After 65 seconds, add 1L of water to the system to precipitate the mulberry branch powder, wash with water 2 to 3 times to remove the ionic liquid (the aqueous solution of the ionic liquid can be distilled to remove water to a certain concentration and can be reused), and place it in a blast drying oven at 75°C in until dry to obtain the initial sample;

[0046] (3) Put the initial sample obtained in step (2) into a crucible, place i...

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Abstract

The invention discloses a method for preparing simulated cellulase. The method comprises the following steps: taking a cellulose material, washing, removing the impurities, drying, grinding, and screening, thereby obtaining cellulose powder; uniformly mixing and stirring the obtained cellulose powder with ionic liquid according to a mass ratio of 1-20:100, preserving the temperature, performing assisted microwave treatment so as to expand the fibers, washing to remove the ionic liquid, and drying to remove the moisture, thereby obtaining a primary sample; adding the obtained primary sample into a crucible, putting the crucible into a tube furnace, and performing heating treatment under gas protection; adding a mixed acid, adding water and washing to remove the impurities, and drying to obtain dried powder, thereby obtaining the sample powder; adding the obtained sample powder into an organic base, stirring, heating, reacting, and washing by using a cleaning solvent, thereby obtaining the simulated cellulase. The simulated cellulase prepared by the method is high in binding ability with a substrate (a cellulose material) and good in thermal stability, high-temperature catalysis can be performed, the catalytic effect is good, the cost of the raw materials is low, and the cellulase can be repeatedly utilized.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and in particular relates to a preparation method of simulated cellulase. Background technique [0002] The research process of simulating enzymes is to use synthetic polymers to simulate the structure, characteristics, principle of action of enzymes and the chemical reaction process of enzymes in organisms. Enzyme is a kind of protein with catalytic activity, which has the characteristics of high catalytic efficiency, strong specificity and mild action conditions. Enzymes are easily inactivated by physical, chemical and other factors, so enzymes cannot be widely used to replace industrial catalysts. The main purpose of studying simulated enzymes is to solve the above shortcomings of enzymes and greatly enhance the stability of enzymes. At present, the research on mimetic enzymes mainly includes the following five aspects: [0003] The metal prosthetic group of the simulated enzyme: the met...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J31/06C08B15/00
Inventor 李强张大钊季更生屠洁李天程
Owner JIANGSU UNIV OF SCI & TECH
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