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Establishing method of Cnobilis inbred line

The invention relates to a technology for the establishment of a scallop and a method for establishing the scallop, which is applied in the field of establishing an inbred line of the scallop Huagui, and can solve the problems of no artificial insemination method, so as to achieve the effects of speeding up the breeding of varieties, speeding up the speed of purification, and being easy to operate.

Active Publication Date: 2012-05-09
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Problems solved by technology

Although the existence of hermaphroditism has been observed through tissue sections in Chlamys chinensis, there is no report on the method of using this phenomenon to construct an inbred line, and there is no report on the method of artificial insemination by dissection

Method used

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Embodiment Construction

[0015] November 2009

[0016] 70 two-year-old scallops with full gonads and orange color were selected from the breeding population in Dapeng Bay, and they were kept indoors for 3 days. During the breeding period, the water is changed once a day, the water temperature is 22°C, and sufficient bait is fed to promote the further development of the gonads into a physiologically mature state. Arrange and number the shellfish, puncture the gonad tissue of each shellfish with a syringe needle, smear it on a glass slide and drop a drop of seawater, put the slide glass under a microscope and observe, it is found that there are both eggs and sperm in the gonad tissue of 4 shellfish . The 4 hermaphroditic individuals were dried in the shade for 60 minutes and then stimulated with running water for 30 minutes, and then the shellfish were placed in 4 numbered glass beakers filled with 1L seawater (26°C, warming up 4°C).

[0017] Individuals 1, 2, and 4 began to ejaculate in about 10 minu...

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Abstract

The invention relates to an establishing method of a Cnobilis inbred line, which can quickly purify the genome of the Cnobilis, so as to accelerate variety breeding. Individuals containing female and male germ cells and appearing as female, namely hermaphrodites, are selected through micrographic examination, an egg is fertilized by sperm in the same individual through induced parturition hastening or dissection fertilization, the amount of the sperms are increased or reduced artificially by taking measures to artificially interfere and adjust the relative proportion of the male and the female gametes in the water, and hatching, breeding and cultivating are carried out according to the ordinary technology of the species, so as to establish the inbred line. The establishing method of the Cnobilis inbred line is simple to operate, requires no special chemical reagent, and is easy to popularize and apply.

Description

technical field [0001] The invention belongs to the technical field of shellfish breeding, and in particular relates to a method for establishing an inbred line of Chlamys farreri. Background technique [0002] Chlamys (Minachlamys) nobilis (Reeve, 1852)] is a warm-water shellfish that is naturally distributed in Japan's Honshu, Shikoku, Kyushu, China's South China Sea and Indonesia. It is distributed in Haimen, Zhelang, Daya Bay, Dapeng Bay, Zhapo and other places along the coast of Guangdong Province. Chlamys scallops are dioecious marine bivalve molluscs. They reach sexual maturity in about one year. The female gonads are orange and the male gonads are milky white. The breeding season is from May to October in the coastal areas of Guangdong. In the peak period, 3-15 million eggs can be laid. Hermaphrodite and sex conversion are widespread. The proportion of sexual transformation within one year accounted for 4.47% of the total, among which the proportion of males was 5...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 何毛贤刘文广
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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