Technique for preparing hermaphrodite scallop interspecific hybrid fingerlings
A technology of scallop and body shape, which is applied in the field of preparation of hermaphroditic scallop interspecific hybrid seedlings, can solve problems such as difficulty in summer and overwintering, inability to breed, and narrow temperature adaptation range of purple scallop, and achieves large heterosis, easy purification, and high fertilization rate high effect
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Embodiment 1
[0012] Example 1: After raising the temperature of purple scallops and bay scallops to promote ripening to reach sexual maturity, 18 mature purple scallops and bay scallops were taken respectively, and sperm eggs were obtained after stimulating oviposition and ovulation. The sperm and eggs of two kinds of scallops were cross-fertilized to obtain 4 combined populations of Chunzi (ZZ), Chunhai (HH), Zihai (ZH) and Haizi (HZ), and the fertilization rate of each group was counted. The experimental results showed that the sperm and eggs of purple scallop and bay scallop had higher compatibility, and the fertilization rate of each hybrid group was higher than that of bay scallop, but slightly lower than that of purple scallop selfing group.
Embodiment 2
[0013] Example 2: After obtaining 4 combined populations of pure purple (ZZ), pure sea (HH), purple sea (ZH) and sea purple (HZ) as in Example 1, cultivate them at 22-23 degrees, carefully observe and compare Embryo development in each group. The results showed that the embryonic development of the purple scallop was faster than that of the bay scallop, and the development of the two hybrid combinations was intermediate between the two parents. There were also differences in the size of D-type larvae at 24 hours after fertilization, and the 24-hour D-type larvae of bay scallops were larger than those of purple scallops.
Embodiment 3
[0014] Embodiment 3: after obtaining pure purple (ZZ), pure sea (HH), purple sea (ZH) and sea purple (HZ) total 4 combined populations as in embodiment 1, follow up and measure the growth situation of each group of larvae, it can be seen that The growth rate of the purple scallop was slower than that of the bay scallop (p<0.001, ANOVA), and the growth of the hybrid group was between the two female parents at the beginning, but the growth rate increased with age, showing a female parent effect and increased hybrid Advantage.
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