72results about How to "Increase perversion rate" patented technology

The invention relates to an ecological breeding method of quasipaa spinosas. The method comprises the steps of strain selection, hybridization, egg laying, hatching, pollywog culturing and young quasipaa spinosa breeding. The ecological breeding method has the advantages that three quasipaa spinosa strains bred by the method have great egg laying amount and fast growing speed; the quasipaa spinosas are fed at the temperature of over 12 DEG C in winter to reduce inner energy consumption in winter and maintain good development of sex glands of the quasipaa spinosas; water current stimulates the quasipaa spinosas to lay eggs, which increases egg laying rate and hatching rate of breeder quasipaa spinosas; and according to the method, quasipaa spinosa breeding ponds can prevent escape, diseases and natural enemies and are propitious to increase survive rate, thereby realizing pollution-free ecological breeding of the quasipaa spinosas. The ecological breeding method of the quasipaa spinosas is provided with breeding ponds for drip egg-laying breeding and winter food supplementing and simulates ecological environment.

The invention provides a preparation method and application of a sea bdellovibrio bacteriovorus ecological preparation, and relates to a sea microbial ecological preparation and a preparation method of a bdellovibrio bacteriovorus preparation for mariculture. The preparation method comprises the following steps of: preparing a host bacteria suspension; separating and purifying bdellovibrio bacteriovorus; and propagating and culturing bdellovibrio bacteriovorus. The preparation method is characterized in that after pseudomonas stutzeri is connected with an incline and is activated, adding physiological saline in a test tube to prepare the bacterial suspension; then propagating and culturing with nutrient bouillon; adding a flocculating agent in the obtained bacterial suspension; standing and precipitating after uniformly shaking; adding the physiological saline to obtain 1012 cfu / mL of host bacteria suspension; separating and purifying the bdellovibrio bacteriovorus of collected sea and bottom sediment samples with a double agar plate method; pouring culture media containing the host bacteria suspension and the collected samples into sea agar in the lower layer for culture after uniformly mixing; and adding the host bacteria and the bdellovibrio bacteriovorus obtained by separation into sterilized natural sea, and culturing until the concentration of the bdellovibrio bacteriovorus is 108 to 109 pfu / mL for future use.

The invention relates to the field of Rapana venosa artificial breeding, in particular to a Rapana venosa ecological breeding method. The method comprises: transferring egg bags into a plastic basket or net cage (10-20mesh) for suspended hatch in a nursery pond and charging air continuously, wherein the density of the egg bags is 5,000 to 8,000 / m<3>; breeding larvae at a density of 1 / ml with bait made of chlamydomonas (diatom and tetraselmis are preferred) in an amount of 1.0 to 12.0*10<4> / ml every day at a water temperature of 22 to 26 DEG C; continuously charging air, changing 1 / 2 of water every day; and when the larvae grow three whorls to a size of 700 to 800 micrometers, placing the larvae in to 60-mesh net bags, cages or tanks together with double-shell spats (animal baits for transformed Rapana venosa) for sea breeding.

The invention relates to a treatment method of middle-late stage attaching bases in shellfish seedling breeding, in particular to a method for treating the attaching bases (mesh sheets, red palm ropesand the like) of shellfish (scallops, mussels, arca inflata reeves and the like) by using the benthic diatom film method, thereby greatly improving the larval attaching rate and the metamorphosis rate. The method has convenient operation and can treat a large number of attaching bases. The treatment method and the attaching bases after treatment have no pollution and are safe and environment-friendly; more importantly, the seedling attaching effect is significantly better than the attaching bases treated by using the traditional method.

The invention relates to a method for culturing a Stichopus japonicus seed, which belongs to the technical field of a Stichopus japonicus seed culturing method and comprises the following steps of (1) fishing and catching; (2) screening; (3) transporting and (4) culturing the Stichopus japonicus seed. According to the method for culturing the Stichopus japonicus seed disclosed by the invention, the culturing process is easy to operate, the Stichopus japonicus seed is scientifically selected and fished and caught in advance; the Stichopus japonicus seed can be matured in advance and lay eggs by utilizing the method of indoor heating and accelerating, thus, large-scale seeds can be cultured in the same year, and the produced Stichopus japonicus seed has enhanced hereditary capacity, strong resistance and good property; moreover, as the feed of the Stichopus japonicus seed and the water temperature and the water quality in the Stichopus japonicus culturing pond are strictly controlled, the fecundity of the Stichopus japonicus is better than that of the Stichopus japonicus cultured in the pond and the intertidal zone, thus, the laid eggs have good quality and the distortion ratio at each stage is high.

The invention discloses a preparation method of a Crassostrea hongkongensis fixation medium. The preparation method comprises the following steps that (1) a plurality of rectangular plastic sheets are made and a round hole with the radius being 3-5 mm is formed in each plastic sheet, wherein the length of the plastic sheets is 10-20 cm, the width of the plastic sheets is 2-5 cm and the thickness of the plastic sheets is 1-3 cm; (2) the plastic sheets are strung through a rope, a plastic tube is arranged between every two adjacent plastic sheets, the plastic tubes are arranged on the rope in a sleeved mode, and then the preparation process of the Crassostrea hongkongensis fixation medium is completed. The invention further discloses the Crassostrea hongkongensis fixation medium and an application method of the Crassostrea hongkongensis fixation medium. After being processed through oxalic acid, fresh water and the like, the Crassostrea hongkongensis fixation medium is used as an eyespot larva collection fixation medium; the Crassostrea hongkongensis fixation medium increases the metamorphosis rate of Crassostrea hongkongensis larvas, accelerates juvenile mollusk growth, improves the juvenile mollusk survival ability and increases the juvenile mollusk yield, has the advantages of being capable of being recycled multiple times, light, convenient to use, good in corrosion resistance and the like and is worthy of popularization and application.

The invention relates to a litopenaeus vannamei parent prawn feed capable of replacing a fresh and living bait, and belongs to the field of nutrients and feeds of aquatic animals. The feed comprises the following ingredients in percentage by weight: 40% of fish meal, 6% of casein, 10% of krill meal, 15% of mussel meal, 14.75% of high-gluten flour, 0.5% of cholesterol, 2% of phospholipid, 7% of fish oil, 1% of choline, 0.5% of astaxanthin, 0.75% of vitamin C, 1.5% of calcium hydrogen phosphate, 0.5% of complex vitamins, and 0.5% of composite mineral substances. According to the litopenaeus vannamei parent prawn feed disclosed by the invention, through a compounding ratio of various nutrient components and through selection of raw materials, a prepared compound feed can meet nutritional requirements of parent prawns for reproduction, and feeding effects in respects of an egg laying rate, a continuous egg laying rate, a hatching rate, a distortion rate, egg diameters and the like of the parent prawns fed with the litopenaeus vannamei parent prawn feed are not significantly different from those fed with fresh and living clamworms, so that the feed disclosed by the invention can replace the fresh and living bait for strengthening the nutrients of the parent prawns, and the problem that the parent prawns are infected with external disease sources when being fed with the fresh and living bait is solved.

The invention belongs to the field of aquaculture, and particularly relates to a cultivation method of quasipaa spinosa tadpole metamorphosis larvae. The method comprises a tadpole cultivation pool, ambient conditions, stocking of small tadpoles, a culture method and a method for promoting metamorphosis. The illumination intensity suitable for a tadpole breeding stage, the illumination intensity suitable for a tadpole metamorphosis stage and the illumination intensity for controlling the metamorphosis speed are optimized, so that according to the growth stage and the metamorphosis stage of tadpoles, characteristic nutrition is optimized, and a water quality control method of combination of quicklime water and slow flowing water is optimized; for the tadpoles which are hatched in spring, conditions of illumination, water temperature, nutrition and the like are optimized, so as to shorten the growing period of the tadpoles; for the tadpoles which are hatched in autumn, corresponding conditions of illumination, water temperature, nutrition and the like are also optimized, so as to extend the growing period of the tadpoles; the growing development and metamorphosis development of the tadpoles are promoted, and diseases can be prevented, thereby increasing the survival rate and metamorphosis rate of the tadpoles.

An inductor for the metamorphosis of larvae of dual-shell shellfish is a mixture of KCl, 5-hydroxytryptamine (3.814-387.4 g / m3). Its advantge is high metamorphosis percentage.

The invention discloses a Chlamys nobilis offspring seed cultivation method. Offspring seeds are pretreated by water and are performed with parent Chlamys nobilis selection, induction for oviposition and artificial insemination to cultivate Chlamys nobilis offspring seed larva. The invention adjusts water quality by processing water for the offspring seed before inputting larva, thus effectively improving larva survival rate and distortion ratio, and lowering production cost.

The invention provides a special compound feed for breeding parent prawns of penaeus monodon. The feed comprises the following components in percentage by weight: 25%-40% of fish meal, 5%-9.5% of artemia powder, 3%-6% of enzymolysis fish soluble pulp powder, 3%-9% of schizochytrium limacinum powder, 1%-3% of kelp powder, 2%-8% of hermetia illucens powder, 2%-5% of shrimp shell powder, 6%-12% of mussel powder, 2%-5% of marine red yeast dry powder, 10%-15% of flour, 1.5%-3% of lysolecithin, 0.8%-1.4% of ARA purified oil, 1.5% of DHA purified oil, 1.5% of EPA purified oil, 3.1%-3.7% of stearic acid triglyceride, 1%-2% of fish oil, 1%-5% of monocalcium phosphate, 0.9%-1.22% of vitamin premix and 1.08%-1.4% of mineral premix. According to the special compound feed, the growth performance of the parent prawns of penaeus monodon in the breeding period is remarkably improved, gonad development of the penaeus monodon is promoted, and the egg laying amount, the egg laying rate, the continuous egg laying rate, the fertilized egg hatching rate, the metamorphosis rate and the egg diameter of the parent prawns are increased.

The invention relates to a method for inducing settlement and metamorphosis of capitulum mitella cypris larvae by kinoprene dripping method stepped flow. According to the method, firstly, dissolutionis promoted according to the weight volume ratio of 1:(45-60) (w / v) of kinoprene to dimethyl sulfoxide, and ethanol is added to prepare mutagenic agents containing 5-8mg / mL kinoprene. After a pond A and a pond B of a stepped culture pond are filled with clean sea water, the cypris larvae are transferred into the sea water in the pond A and the pond B of the stepped culture pond, and continuous micro-inflation is performed. Afterwards, a mutagenic agent dripping pipe is opened to continuously drip the mutagenic agents to the pond A. A water feeding pump of the stepped culture pond is started toinject water, and continuous water injection time is 4-6 days. 4-6 days after, water injection and dripping are stopped, cypris larva settlement bases are taken out, and then the cypris larvae can betransferred into a larva breeding stage. The method is simple in operation, less in energy consumption, low in cost, stable in function and remarkable in inducing effect, metamorphosis rate can reach80% or more, and the method is a good method for inducing settlement and metamorphosis of the capitulum mitella cypris larvae.

The invention discloses rana spinosa tadpole feed. The rana spinosa tadpole feed comprises artificial compound feed, vegetable pumpkin feed and mixed feed, wherein the mixed feed comprises the artificial compound feed and the vegetable pumpkin feed according to a weight ratio of 1: 1. After adoption of the rana spinosa tadpole feed, tadpoles accumulate more nutrition, and metamorphose into frogs with larger body shapes, so that the survival potential can be further improved and the survival rate can be increased; the tadpoles are omnivorous, and full compound feed meets food habits of the tadpoles and nutrients needed during growth, so that improvement on the disease resistance of the tadpoles is facilitated, and the tadpoles are high growth speed, long in total length and high in metamorphosis survival rate; through increase in the metamorphosis rate and the disease resistance, the growth cycle of the tadpoles is shortened to about 90 days, and the growth cycle is short. The rana spinosa tadpole feed and the feeding method thereof have the characteristics of capability of greatly increasing the survival rate, the total length and the metamorphosis survival rate of the tadpoles and great reduction in the feeding cost, and have the advantages of a short feeding cycle and strong disease resistance of the tadpoles.

The invention relates to a method for increasing pinctada maxima larva metamorphosis rate, and belongs to the technical field of shellfish culture. The method includes the specific steps: (1) preparing 70-100cm long palm ropes with the diameters of 2-3cm, soaking the palm ropes in 1% potassium permanganate solution for 10-12 hours, soaking the palm ropes in fresh water for 10-12 hours and cleaning residual potassium permanganate solution; (2) soaking the palm ropes in an outdoor cement pond for 1-2 days, continuously adding natural seawater with the quantity of 10-15L / min, and washing out sludge impurities attached to the palm ropes by filtered seawater after soaking; (3) placing 20-30 palm ropes into a nursery pond per m<3>; (4) transferring an attaching base to another nursery pond for culture after settlement and metamorphosis of larvae for 5-7 days; (5) placing the attaching base into an 80-mesh net bag and transferring fingerlings into a natural sea area for culture when the sizes of the fingerlings on the palm ropes reach 2-3mm. The proportion of eyebot larvae in the nursery pond is 50-70%, and the larva density is 0.5-1.0 piece / mL. The pinctada maxima larva metamorphosis rate and fingerling culture survival rate can be obviously increased, and the method is low in cost, simple and convenient.

The invention discloses a large-scale breeding method and equipment for hybrid young scallops. Through temperature regulation and other technical means, gonads of bay scallops and gonads of patinopecten yessoensis both of which become mature at the same time lay eggs and produce sperms respectively; then, a stepwise cooling mode is adopted for lowering the temperature of egg laying and spermiationwater for the bay scallops to be suitable for patinopecten yessoensis to lay the eggs, and then the egg laying and spermiation water for the bay scallops is mixed with the egg laying water for patinopecten yessoensis. The vitality of the sperms is maintained, the fertilization rate, hatching rate, survival rate and metamorphosis rate of planktonic larvae in a hybridization process are increased,and the production efficiency of the hybrid young scallops is improved. The hybrid young scallops also have the advantages of being high in growth speed, wide in temperature-resistant range, high in adult scallop yield and the like.

The invention provides a cultivation method of panulirus stimpsoni larva, which comprises the steps of: A. cultivating the hatched panulirus stimpsoni by a first bait for 13-17 days, wherein the first bait comprises at least one of the artemia nauplii, the scallop fertilized eggs, the oyster fertilized eggs and the planktonic copepodid larva; B. cultivating the panulirus stimpsoni by a second bait for 8-12 days, wherein the second bait comprises the first bait of the step A and the copepodid adult; and C. continuously cultivating the panulirus stimpsoni larva till that the panulirus stimpsoni larva is completely mutated into thelarva, wherein the bait comprises a third bait, and the third bait comprises the mural copepodid adult. The cultivation method of the panulirus stimpsoni larva can cultivate the panulirus stimpsoni larva in bulk, and is high in survival rate, high in mutation rate, and high in growth speed.

The invention provides a healthy aquatic microcapsule biological feed which is prepared by biochemically-treated raw materials, namely, composite high protein power, algae single-cell extractive, microbial protein yeast, detoxification and fermentation potato powder, a micro-ecology nutrient solution and a food calling coating agent by a curing material production device, a micro-ecology nutrient solution emulsifying mixing pelletizing device and a coating layer coating microcapsule production device. The quality of the feed can be improved, the production cost can be reduced, and the safety probability of the food can be improved since the chemical antibiotics additive is replaced by the biological product; the nutrition ingredients of the raw materials of the feed can be protected due to a microcapsule synthesis production technology; the gustatory difficulty of seedling feeding can be solved due to the food calling coating agent; and the feed is persistent in water due to the microcapsule, so that the pollution of the feed residue to the water quality can be reduced.

The invention discloses a method for cultivating sea cucumber larvae. The method includes steps of distributing sea cucumber fries and cultivating the larvae; feeding mixed liquid fodder containing simulated fine nannochloris oculata dry powder, yeast and EM (effective microorganisms) to the larvae after larva embryos are developed into the small auricularia larvae and the digestive tracts of the small auricularia larvae are completely developed; enabling the auricularia larvae to start to be gradually developed into doliolarias at the end of the fifth feeding day; completing sea cucumber larva cultivation after the eighth feeding day; starting a juvenile sea cucumber cultivating stage. Compared with the traditional method for cultivating sea cucumber larvae, the method for cultivating the sea cucumber larvae has the advantages that the method is simple and convenient, fodder cultivating workshops can be omitted, labor and materials can be saved, the production cost can be reduced, and the fodder can be stably supplied, is safe and is comprehensively nutritional.

The invention discloses a bait formula for enhancing nutrition of meretrix lamarckii at a planktonic larva phase. The bait formula comprises the following raw materials in parts by weight: 3-7 parts of a chlorella mother liquid, 0.5-0.8 part of a isochrysis galbana parke mother liquid, 0.6-0.9 part of a chaetoceros mulleri mother liquid, 0.05-0.6 part of a platymonas subcordiformis mother liquid and 0.02-0.06 part of a rhodotorula benthica mother liquid. The bait formula for enhancing nutrition of meretrix lamarckii at the planktonic larva phase, which is disclosed by the invention, has comprehensive and sufficient nutrition, is good in palatability, excellent in food calling and intake effect, capable of meeting requirements of enhanced nutrition and high-metamorphosis survival rates at the larva phase, improving the immunity and the stress resistance of zooplankton, improving disease and insect resistance of the zooplankton and purifying water, and has rich bacterial with high activity and metabolite with great gain, and development by metamorphosis of planktonic larvae can be accelerated.

A metamorphosis inductor based on organic substances for the larvae of dual-shell shellfish is prepared from KCl (5.0-1000 g / m3, L-tyrosine (0.01-3 g / m3 and VB12 (0.01-0.5 mg / m3 through proportionally mixing. Its advantage is high metamorphosis percentage increased by 20-40%.

The invention provides a saline-alkaline tolerant molecular marker C1480 for portunus trituberculatus and an application of the saline-alkaline tolerant molecular marker C1480. The nucleotide sequenceof the molecular marker C1480 is as shown in SEQ ID No. 1, and the nucleotide sequence of a primer pair for detecting the molecular marker C1480 is as shown in SEQ ID No. 2 and SEQ ID No. 3. The molecular marker C1480 is an SNP marker, and the alkali-resistant genotype of the molecular marker C1480 is a GG genotype. The molecular marker C1480 provided by the invention is not limited by the growthstage of portunus trituberculatus; the molecular marker C1480 can be used for obviously accelerating the breeding process of portunus trituberculatus and quickly breeding the portunus trituberculatusspecies with excellent saline-alkaline tolerance, is accurate and reliable in detection of the saline-alkaline tolerance of the portunus trituberculatus by using the molecular marker C1480, is simpleto operate, and is beneficial to healthy breeding and development of the portunus trituberculatus, thereby having wide application prospect.

The invention discloses a method for increasing the metamorphosis adhesion rate of eyespot larvae of pinctada martensi and belongs to the technical field of aquaculture. The method comprises the basicsteps of preparation of an adhesion water pond for the eyespot larvae of pinctada martensi, collection of the eyespot larvae, medicine feeding and young pinctada martensi breeding management; in theprocess of breeding young pinctada martensi, the eyespot larvae without metamorphosis adhesion in a young pinctada martensi breeding pond are collected and moved to a young pinctada martensi breedingpond with fully hung young pinctada martensi collection instruments for cultivation, antibiotics and vitamins are used for improving ingestion and digestion of the eyespot larvae, and the metamorphosis adhesion rate of the eyespot larvae of pinctada martensi is increased. By adopting the method, large-scale manual young pinctada martensi breeding of pinctada martensi is conducted, the metamorphosis adhesion rate of the larvae is high, the individual size of young pinctada martensi is even, the young pinctada martensi breeding yield of pinctada martensi can be increased, and the economic benefit of pinctada martensi can be improved; the method is suitable for large-scale manual young pinctada martensi breeding of pinctada martensi, easy to apply and popularize and obvious in social benefitand economic benefit.

The invention discloses a method for cultivating burying habitat type shellfish larvae. The method comprises the following steps of after shellfish grows to D-shaped larvae, washing young shellfishesinto a cultivation basket, not adding a substrate into the cultivation basket, ensuring that the pond distribution density in the cultivation basket is 10ind / ml, and adhering a 300-mesh bolting silk net to the inner surface of the cultivation basket; putting the cultivation basket into a cultivation water body for cultivation, feeding bait organisms into the cultivation basket at regular time, andmaintaining the concentration of the bait organisms at 5*10<5>ind / ml; introducing oxygen into the cultivation water body to keep the cultivation water body rich in oxygen; and performing regular young shellfish washing and water changing. Compared with the prior art, the method has the following advantages that 1, the cultivation basket is arranged in the cultivation water body, the cultivation environment is improved, and the young shellfish growth speed and survival rate are increased; 2, the substrate does not need to be added, seawater permeates through the 300-mesh bolting-silk net, andthe influence of zooplankton on young shellfishes is reduced; and 3, the young shellfishes are basically not damaged by washing the young shellfishes and changing water, and the labor cost is greatlyreduced.

The invention provides a low-salt-tolerant molecular marker C62 for portunus trituberculatus and application of the low-salt-tolerant molecular marker C62. A nucleotide sequence of the molecular marker C62 is as shown in SEQ ID No.1, and nucleotide sequences of a primer pair for detecting the molecular marker C62 are as shown in SEQ ID No.2 and SEQ ID No.3. The molecular marker C62 is an SNP marker, and the low-salt tolerance genotype of the molecular marker C62 is an AG genotype. The molecular marker C62 provided by the invention is not limited by the growth stage of the portunus trituberculatus, is used for accelerating the breeding process of the portunus trituberculatus and quickly breeding crab species with excellent low-salt resistance, and is beneficial to healthy breeding and sustainable development of the portunus trituberculatus, so that the molecular marker C62 has a wide application prospect.

The invention discloses an artificial cultivation method of red-stripe lysmata vittata larvae. The artificial cultivation method comprises following steps: collection of larvae; density and environmental requirements of larva cultivation; recirculating aquaculture prior to larval metamorphosis and cultivation of larvae. The artificial cultivation method of red-stripe lysmata vittata larvae has following beneficial effects: artificial cultivation is achieved for the first time by adoption of artificial cultivation technology so that survival rate and metamorphosis rate of zoea are greatly increased; and all of these provide quantity and quality assurance for red-stripe lysmata vittata in an aquatic market .

The invention relates to a breeding method capable of controlling the aberration rate and the body color abnormity of verasper variegatus seedlings and belongs to the field of aquatic seedling culture. The method comprises the following steps of: feeding reinforced rotifer to fish larvae of verasper variegatus, which are at the age of 5 to 6 days; when the verasper variegatus grows to the age of 19 to 21 days, simultaneously feeding artemia nauplii and reducing the feeding quantity of the rotifer until the verasper variegatus grows to the age of 34 to 36 days; and then starting to completely feed the artemia nauplii, wherein the artemia nauplii is reinforced by schizochytrium limacinum and the rotifer is reinforced by se-yeast after being reinforced by the schizochytrium limacinum. According to the invention, the Se-yeast is used as a reinforcer of the rotifer; content of selenium in a bait is improved; the fish larvae of verasper variegatus can be promoted to grow; the metamosphsis rate of the fish larvae of verasper variegatus is promoted; the aberration rate and the ratio of the body color abnormity are effectively reduced; survival rate is high; and the seedlings have high quality.

The invention discloses a fermentation type feed capable of promoting metamorphosis of bullfrog tadpoles. The feed comprises the following raw materials in parts by weight of 10-20 parts of fish meal,8-20 parts of chicken meat powder, 20-40 parts of dehulled soybean meal, 4-10 parts of peanut meal, 5-12 parts of corn protein powder, 8-16 parts of rice bran, 16-26 parts of flour, 0.5-2 parts of avitamin premix feed for frogs, 0.5-2 parts of a multiple-mineral premix feed for frogs and 0.2-0.4 part of 50% choline chloride, and further comprises fermentation components by weight of 1-3kg of lactic acid composite microbes, 1-3kg of spore composite microbes, 2-4kg of microzymes and 0.5-1kg of complex enzymes. The invention further discloses a preparation method of the fermentation type feed capable of promoting metamorphosis of bullfrog tadpoles. The metamorphosis cycle of the bullfrog tadpoles can be shortened through improving water quality and increasing the digestion and utilization rate, so that the metamorphosis rate of the tadpoles can be increased.