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Method for increasing pinctada maxima larva metamorphosis rate

A technology for pearl oysters and larvae, which is applied in the field of shellfish culture, can solve the problems of restricting the commercial production of pearl oysters, unable to obtain nucleated mother oysters in batches, and low survival rate of mother oysters, etc. Low, improve the effect of survival rate

Inactive Publication Date: 2017-05-31
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the 1980s, my country successfully cultivated pearl oyster with nuclei, but because the artificial seedlings of pinnata were cultured in sea areas, the survival rate of oysters capable of implanting nuclei was very low, less than 1%, and it was impossible to obtain nuclei in batches. Mother oyster, which seriously restricts the commercial production of pinnacle oyster in my country

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] On April 23, 2012, Leizhou Haiwei Aquaculture Co., Ltd. Using the natural population collected from Weizhou Island in the North Sea, the age of shellfish is 3-4 years old, and the shell length is 16.5-19.3cm. Pick 8 individuals with mature gonads (male gonads milky white, female gonads light yellow), the number of males and females is 5 and 3 respectively, and the attachments such as oysters, barnacles, and lime worms attached to the shell surface are cleaned. Use the shade drying to induce the parents to discharge gametes, and the fertilized eggs are hatched in a 200L plastic basin; the fertilized eggs are hatched for 48 hours, and the floating larvae are transferred to a 1000L glass steel bucket for cultivation, and the larvae density is 0.5 / mL; the larvae are cultivated according to conventional techniques, and the bait is fed twice a day , change the water by 20% every other day; the proportion of eye-spot larvae reaches 50%, and put in the palm rope attachment base...

Embodiment 2

[0023] On April 25, 2013, Leizhou Haiwei Aquaculture Co., Ltd. Breeding group, the age of shellfish is 3 years old, and the shell length is 14.7-17.5cm. Pick 10 individuals with mature gonads (male gonads milky white, female gonads light yellow), the number of males and females is 6 and 4 respectively, and the attachments such as oysters, barnacles, and lime worms attached to the shell body surface are cleaned. Use 0.04% 5-hydroxytryptamine solution to soak the parents for 40mins, fertilized eggs hatch in 200L plastic basins; fertilized eggs hatch for 48h, transfer the floating larvae to a 1000L glass steel bucket for cultivation, and the larvae density is 1.0 / mL; carry out larvae cultivation according to conventional techniques, and throw them every day Feed the bait twice, and change the water by 20% every other day; when the proportion of eye-spot larvae reaches 70%, put in the palm rope attachment base; The number of attachment bases was 20; 7 days after the larvae were a...

Embodiment 3

[0025] On June 12, 2013, Leizhou Haiwei Aquaculture Co., Ltd. Breeding group, the age of shellfish is 3 years old, and the shell length is 15.2-17.8cm. Pick 6 individuals with mature gonads (male gonads milky white, female gonads light yellow), the number of males and females is 4 and 2 respectively, and the attachments such as oysters, barnacles, and lime worms attached to the shell surface are cleaned. Use the shade drying stimulation to induce the parents to discharge gametes, and the fertilized eggs are hatched in 200L plastic pots; the fertilized eggs are hatched for 48 hours, and the floating larvae are transferred to a 1000L glass fiber reinforced plastic bucket for cultivation, and the larvae density is 0.8 / mL; the larvae are cultivated according to conventional techniques, and the bait is fed twice a day , change the water by 20% every other day; the proportion of eye-spot larvae reaches 60%, and put in the palm rope attachment base; 20; 7 days after the larvae were ...

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Abstract

The invention relates to a method for increasing pinctada maxima larva metamorphosis rate, and belongs to the technical field of shellfish culture. The method includes the specific steps: (1) preparing 70-100cm long palm ropes with the diameters of 2-3cm, soaking the palm ropes in 1% potassium permanganate solution for 10-12 hours, soaking the palm ropes in fresh water for 10-12 hours and cleaning residual potassium permanganate solution; (2) soaking the palm ropes in an outdoor cement pond for 1-2 days, continuously adding natural seawater with the quantity of 10-15L / min, and washing out sludge impurities attached to the palm ropes by filtered seawater after soaking; (3) placing 20-30 palm ropes into a nursery pond per m<3>; (4) transferring an attaching base to another nursery pond for culture after settlement and metamorphosis of larvae for 5-7 days; (5) placing the attaching base into an 80-mesh net bag and transferring fingerlings into a natural sea area for culture when the sizes of the fingerlings on the palm ropes reach 2-3mm. The proportion of eyebot larvae in the nursery pond is 50-70%, and the larva density is 0.5-1.0 piece / mL. The pinctada maxima larva metamorphosis rate and fingerling culture survival rate can be obviously increased, and the method is low in cost, simple and convenient.

Description

technical field [0001] The invention relates to the cultivation of pinnata larvae, in particular to a method for increasing the metamorphosis rate of pinnata pinnata larvae, and belongs to the technical field of shellfish cultivation. Background technique [0002] Sessile metamorphosis is an important developmental process in marine bivalve molluscs, during which planktonic larvae lose their veils and change from planktonic to sessile. The attachment substrate is one of the key factors affecting the metamorphosis rate of bivalve larvae. Existing studies have shown that the type, material and color of the attachment substrate have a significant impact on the metamorphosis rate of bivalve larvae. For example, the use of dark-colored (black and brown) attachment substrates can increase the metamorphosis rate of larvae. In addition, studies have shown that metal ions, amino acids, and neuroactive amine chemicals induce metamorphosis in larvae. The main components of biofilm ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/56
CPCY02A40/81
Inventor 邓岳文王庆恒杨创业杜晓东梁飞龙李俊辉
Owner GUANGDONG OCEAN UNIVERSITY
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