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Method for producing L-tyrosine through enzyme method

A tyrosine and enzymatic technology, applied in the field of L-tyrosine preparation and extraction, can solve the problems of low yield and low purity of tyrosine extraction, achieve high yield, overcome long reaction time, and improve production The effect of efficiency and yield

Active Publication Date: 2016-09-28
SHANDONG YANGCHENG BIOLOGY TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the deficiencies in the prior art, the present invention provides a method for enzymatically producing L-tyrosine, which solves the problem of low extraction purity of tyrosine by improving and optimizing the enzymatic reaction steps and extraction process conditions. , the problem of low yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The extraction method of L-tyrosine of the present invention is as follows:

[0032] 1. Preparation of fermentation broth containing L-tyrosine

[0033] (1) Use Torulopsis glabrata CGMCCNO.0879 recorded in the patent CN03109214.4 to ferment and prepare pyruvate for the bacterial strain, the method is: add Torulopsis glabrata to the pyruvate fermentation medium according to the inoculation amount of 10%, Fermentation was carried out under the conditions of 30°C, pH 5.0, dissolved oxygen 30-50%, and a total of 48 hours of fermentation. The concentration of pyruvic acid at the end of fermentation was about 70g / L; the composition of the fermentation medium was: glucose, KH 2 PO 4 , soybean peptone, soybean meal hydrolyzate, ammonium sulfate;

[0034] (2) When 4 hours away from the end of fermentation, add β-tyrosinase to the fermentation broth, and then continuously add phenol and NH 4 Cl, and control the flow velocity so that the flow velocity of phenol in the system is 1...

Embodiment 2

[0044] The extraction method of L-tyrosine of the present invention is as follows:

[0045] (1) Take 35000L of the fermentation broth containing L-tyrosine prepared in Example 1, heat it to 70°C, and adjust the pH to 2-4 with acid until the L-tyrosine is completely dissolved;

[0046] (2) Add 8g / L activated carbon to the fermentation broth, decolorize at 70°C for 60min;

[0047] (3) After decolorization, the fermented liquid is filtered through a ceramic membrane to obtain a decolorized clear liquid. The filtering conditions are: the average pore diameter of the ceramic membrane is 220nm, the temperature is 60°C, the pressure is 0.35Mpa, and the flow rate of the fermented liquid is 60L / m 2 h, the filtration time is 2 hours; the filtered bacteria, protein and activated carbon concentrate are filtered by a high-pressure filter press and then incinerated in a boiler;

[0048] (4) The decolorized clear liquid is filtered through an acid-resistant liquid security filter to effecti...

Embodiment 3

[0052] The extraction method of L-tyrosine of the present invention is as follows:

[0053] (1) Take 35000L of the fermentation broth containing L-tyrosine prepared in Example 1, heat it to 90°C, and adjust the pH to 2-4 with acid until the L-tyrosine is completely dissolved;

[0054] (2) Add 14g / L activated carbon to the fermentation broth, decolorize at 80°C for 30min;

[0055] (3) After decolorization, the fermented liquid is filtered through a ceramic membrane to obtain a decolorized clear liquid. The filtering conditions are: the average pore diameter of the ceramic membrane is 180nm, the temperature is 90°C, the pressure is 0.2Mpa, and the flow rate of the fermented liquid is 150L / m 2 h, the filtration time is 2 hours; the filtered bacteria, protein and activated carbon concentrate are filtered by a high-pressure filter press and then incinerated in a boiler;

[0056] (4) The decolorized clear liquid is filtered through an acid-resistant liquid security filter to effect...

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Abstract

The invention discloses a method for producing L-tyrosine through an enzyme method. The method comprises the following steps of adopting a fermentation method to produce pyruvic acid, adding beta-tyrosinase, phenol and NH4Cl at the fermentation anaphase of the pyruvic acid, converting the generated pyruvic acid into the L-tyrosine, and obtaining fermentation liquor containing the L-tyrosine; heating the fermentation liquor containing the L-tyrosine to be 70 DEG C to 90 DEG C, and acidifying until L-tyrosine crystals are completely dissolved; adding activated carbon to decolor, after decoloring, filtering by utilizing a ceramic membrane, removing bacteria, albumen and the activated carton, carrying out secondary filtering on obtained clear liquid through an acid-resisting liquid cartridge filter; adjusting the pH of the secondary filtered clear liquid to be 5.0 to 7.0, cooling to crystallize, centrifuging, and obtaining the L-tyrosine. According to the method for producing the L-tyrosine through the enzyme method provided by the invention, the process is simplified, the production efficiency is improved, the obtained L-tyrosine is high in purity and yield, the purity of the obtained tyrosine is remarkably improved and reaches to 98.0 percent or above compared with a traditional extraction method, and various tyrosine drug standards are met.

Description

technical field [0001] The invention relates to a method for enzymatically producing L-tyrosine, belonging to the technical field of L-tyrosine preparation and extraction. Background technique [0002] L-tyrosine is an aromatic amino acid, which belongs to a kind of amino acid, which cannot be synthesized by the human body, and is a non-essential amino acid for the human body. In medicine, L-tyrosine is used to treat hyperthyroidism. In addition, L-tyrosine is also the main raw material for the synthesis of polypeptide hormones, antibiotics, L-dopa and other drugs. [0003] There are three methods for preparing L-tyrosine, one is the extraction method, which uses natural protein resources such as human hair, pig hair, pig blood meal, etc. tyrosine. Since the protein raw material hydrolyzate contains a variety of amino acids, among which the isoelectric point and solubility of L-tyrosine and L-cystine are similar, it is very difficult to separate and obtain high-purity L-ty...

Claims

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Application Information

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IPC IPC(8): C12P13/22
CPCC12P13/22
Inventor 王东阳蔡传康刘朋朋张华陈正
Owner SHANDONG YANGCHENG BIOLOGY TECH CO LTD
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