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Method for ecologically interplanting sarcandra glabra under phyllostachys edulis forest

A technology for coral and moso bamboo, applied in the fields of botanical equipment and methods, horticulture, application, etc., can solve the problems of low maturity rate and harvest rate of grass coral seeds, seasonal restrictions on materials, difficult seed sources, etc., and achieves simple and easy breeding. , Conducive to management and improve the effect of land utilization

Inactive Publication Date: 2013-06-12
浙江慧普农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Publication number is that the Chinese patent of CN 101194593A has introduced a kind of tissue culture fast propagation method of Coral praecox, this method uses the root system of aseptic seedling as explant to obtain regenerated seedling through the callus approach, but this method must use Coral pratense without The test-tube seedlings obtained by sowing bacteria or inducing axillary buds of stem segments are used as test materials, the steps are cumbersome, the production cycle is long, and there are still problems of low maturity rate and harvest rate of grass coral seeds, difficult seed sources, seasonal restrictions on material selection and low germination rate.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Group cultivation of seedlings

[0032] Select healthy and disease-free young leaves of Coralia praecox as explants, soak them in 75% alcohol for 20 seconds in an ultra-clean workbench, then soak them in 0.1% mercuric solution for 1 minute, rinse them with sterile water 3 to 4 times, and cut them into areas about 1cm 2 Left and right small squares, spare. The sterilized leaves were placed on the adventitious bud induction medium with the adaxial side up, and transferred to fresh medium for 30 days after inoculation. The adventitious bud induction medium is based on MS as the basic medium, supplemented with 2.5 mg / L TDZ, 0.15 mg / L NAA, 120g / L banana puree and 0.8g / L activated carbon; Swelling and green bud spots appeared, followed by regeneration of adventitious buds. When cultured for 30 days, the regeneration frequency and average number of adventitious shoots were 82.1% and 5.3, respectively. The adventitious buds growing from the edge of the leaves were cut i...

Embodiment 2

[0044] (1) Group cultivation of seedlings

[0045] Select healthy and disease-free young leaves of Coralia praecox as explants, soak them in 75% alcohol for 20 seconds in an ultra-clean workbench, then soak them in 0.1% mercuric solution for 1 minute, rinse them with sterile water 3 to 4 times, and cut them into areas about 1cm 2 Left and right small squares, spare. The sterilized leaves were placed on the adventitious bud induction medium with the adaxial side up, and transferred to fresh medium for 35 days after inoculation. Adventitious bud induction medium is based on MS as the basic medium, supplemented with 2.0 mg / L TDZ, 0.1 mg / L NAA, 150g / L banana puree and 0.5g / L activated carbon; Swelling and green bud spots appeared, followed by regeneration of adventitious buds. When cultured for 30 days, the regeneration frequency and average number of adventitious buds regenerated from explants were 80.2% and 4.9, respectively. The adventitious buds growing from the edge of th...

Embodiment 3

[0055] The operating steps and parameters of this embodiment refer to Example 1. The difference is that the leaf adventitious bud induction medium is based on MS as the basic medium, and 4.0 mg / L TDZ, 0.2 mg / L NAA, 100 g / L banana puree and 1.0g / L activated carbon; 15 days after the leaves were inoculated into fresh medium, the incisions were slightly enlarged and green buds appeared, and then adventitious buds began to regenerate. When cultured for 30 days, the regeneration frequency and average number of adventitious buds regenerated from explants were 78.4% and 4.7, respectively. The adventitious buds growing from the edge of the leaves were cut into individual buds and inoculated in the proliferation medium, which was based on MS, supplemented with 2.0 mg / L TDZ and 0.05 mg / L NAA. The same proliferation medium can be used for multiple subcultures, once every 25 to 30 days, and the average multiplication factor of clustered buds is 6.0. Cut out a single bud with a heigh...

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Abstract

The invention relates to a method for ecologically interplanting sarcandra glabra under a phyllostachys edulis forest. The method comprises the steps of: (1) tissue culture and seedling raising: sarcandra glabra blades are selected as explants to conduct adventitious bud induction, an adventitious bud induction culture medium uses an MS (Murashige and Skoog) culture medium as a basic culture medium and additionally comprises 2.0 to 4.0mg / L of TDZ (thidiazuron), 0.1 to 0.2mg / L of NAA (naphthyl acetic acid), 100 to 150g / L of banana puree and 0.5 to 1.0g / L of activated carbon, and then adventitious buds are subjected to enrichment culture and rooting culture; (2) seedling hardening: bottom seedlings are conveyed to under the phyllostachys edulis forest to be hardened; (3) transplantation: tissue culture seedlings are transplanted to a north slope bamboo forest with a number of living stems (NLS) of phyllostachys edulis of 150 to 400 plants / mu or a south slope bamboo forest with an NLS of 200 to 400 plants / mu; (4) forest management: the second intertillage is conducted in June and September every year; and (5) harvest: the sarcandra glabra is harvested in November to December every year. By adopting the method, the culture period of the sarcandra glabra can be shortened and the yield of the sarcandra glabra can be increased.

Description

technical field [0001] The invention belongs to the technical field of agricultural planting, and specifically relates to a method for ecologically interplanting grass and coral under moso bamboo forests. Background technique [0002] Grass coral (Sarcandra glabra (Thunb.) Nakai), alias: Sarcandra glabra (Thunb.) Nakai, alias: Sarcandra glabra (Thunb.) Nakai, etc., is a perennial evergreen semi-shrub of the genus Sarcandra glabra (Thunb.) Nakai. The whole plant is used for medicine. It has the functions of antibacterial and anti-inflammatory, clearing away heat and detoxification, expelling wind and dampness, promoting blood circulation and relieving pain, promoting menstruation and setting bones, etc. , has a remarkable effect on a variety of malignant tumors, and is an excellent Chinese medicinal material with a wide range of pharmacological effects and low toxicity. With the continuous development of new products that use coral as raw materials, the demand for them is in...

Claims

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Application Information

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IPC IPC(8): A01G1/00
Inventor 朱淑颖姜叶琴张海洋
Owner 浙江慧普农业科技有限公司
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