Molecular marker fingerprinting for identifying anoectochilus formosanus produced at Huoshan in Anhui and similar species thereof

Abstract

The invention discloses a molecular marker fingerprinting for identifying the anoectochilus formosanus produced at Huoshan in Anhui and the similar species thereof. The molecular marker fingerprinting comprises the steps of preprocessing anoectochilus formosanus samples, extracting DNA of each sample genome, executing ISSR-PCR (inter-simple sequence repeat-polymerase chain reaction), executing agarose gel electrophoresis, building ISSR molecular marker fingerprint of each sample and using the ISSR molecular marker fingerprint for identifying the anoectochilus formosanus germplasm. The method provided by the invention saves time and economic cost, and can obtain the accurate and reliable identification fingerprint just by extracting the DNA of the anoectochilus formosanus plants, executing ISSR-PCR and executing agarose gel electrophoresis; the method identifies the species, which are easy to confuse in appearance, from the essence of inheritance, has the advantages of being simple, convenient and efficient, excellent in repeatability and high in resolution, and so on; and the method can identify the species in seeding stage of plants, and plays an important role in guaranteeing the accuracy and the stability of the Huoshan anoectochilus formosanus crude drug base source.

Description

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AI Technical Summary

Benefits of technology

This patented technique allows us to create genetic markers that help research scientists better study crops or other organisms' roots based on their natural characteristics such as size, shape, coloration, etc., without having them undergo physical damage during testing. It also helps predict how different types of medicine will work well together when mixed together due to similar properties from each type. Overall, this new discovery makes it possible to improve agricultural production efficiency while reducing costs associated therewith.

Problems solved by technology

The technical problem addressed in this patents is how to accurately distinguish various types of citrus crops like Xylonglavescenceceae (Coriidae). Existing methods require expensive equipment and complex processes, making them difficult to use outside China where they may affect healthy populations.

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