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Method for promoting germination and seedling formation of anoectochilus formosanus seeds

A technique for clematis and seeds, which can be applied in the directions of seed immunity, seed and rhizome treatment, and microorganism isolation, and can solve problems such as low seedling efficiency.

Inactive Publication Date: 2017-06-20
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the technical defects of the prior art, and provides a method for promoting the germination and seedling formation of clematis seeds, so as to solve the technical problem in the prior art that the natural germination and seedling growth efficiency of clematis seeds is low

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  • Method for promoting germination and seedling formation of anoectochilus formosanus seeds

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1 material

[0024] Wild clematis, purchased from Tonggu County, Jiangxi Province

[0025] 2 Research Methods

[0026] 2.1 Acquisition of endophytic fungi

[0027] Harvest the roots of wild clematis, wash off the sediment with tap water, soak in soapy water for 10-20 minutes, rinse well, disinfect the surface with 75% alcohol for 30 seconds, disinfect with 0.1% mercury liter for 5 minutes, and finally rinse with sterile water 5 times. Each root was cut into several 0.5-1.0cm root segments, and placed flat on the potato dextrose agar medium (PDA plate medium, sterilized in advance, subpackaged in sterile petri dishes), and each petri dish was connected with 5 root segments. The control group was the last cleaning solution, which was applied to petri dishes, and 40 petri dishes were inoculated, sealed with a parafilm and placed in a constant temperature incubator at 28°C for dark cultivation.

[0028] After the bacteria grow out, they are purified by streaking. On th...

Embodiment 2

[0039] The invention discloses a method for promoting the germination of clematis seeds. The method comprises the steps of isolating fungi from roots of clematis plants, and co-cultivating the obtained fungi and sterilized clematis seeds in a sterile substrate.

[0040] The isolation of fungi from the roots of clematis plants includes the following steps: take wild clematis roots, wash and disinfect the surface with alcohol with a concentration of 75% for 30 seconds, then sterilize with 0.1% mercury liter for 5 minutes, and then wash with aseptic water clean; put the 0.5cm-long root section flatly on the sterile PDA plate medium, seal it with a parafilm and culture it in the dark at 28°C; pick the colony, which is the isolated fungus.

[0041] The sterile matrix contains wood chips.

[0042] The aseptic matrix is ​​prepared by the following method: take the fermented sawdust matrix, add distilled water to moisten it, divide it into culture bottles for sterilization, and cool d...

Embodiment 3

[0045] The invention discloses a method for promoting the germination of clematis seeds. The method comprises the steps of isolating fungi from roots of clematis plants, and co-cultivating the obtained fungi and sterilized clematis seeds in a sterile substrate.

[0046] The fungus isolated from the root of the clematis plant comprises the following steps:

[0047] 1) Take the root of wild clematis, wash it, disinfect it with 75% alcohol for 30 seconds, then disinfect it with 0.1% mercury chloride for 5 minutes, and then wash it with sterile water; put the 1cm-long root on a sterile On the PDA plate medium, after sealing with a parafilm, culture in the dark at 28°C;

[0048] 2) pick colonies and purify by streaking to obtain several purely cultured colonies of different species;

[0049] 3) Pick the colony of Rhizoctonia solani from the several colonies obtained in step 2), which is the isolated fungus.

[0050] The aseptic matrix is ​​prepared by the following method: take the...

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Abstract

The invention provides a method for promoting germination and seedling formation of anoectochilus formosanus seeds. According to the technical scheme, an idea that the anoectochilus formosanus seeds can germinate only through symbiosis with fungi is found by virtue of an experimental measure, a set of fungus acquiring methods and symbiosis culture conditions are explored under the guidance of the idea, meanwhile, a strain capable of outstandingly promoting the germination and seedling formation of the anoectochilus formosanus seeds on a sawdust matrix is definitized. Particularly, the method comprises the steps of separating endophytic fungi from the root of wide anoectochilus formosanus, mixing a mixed fungal colony or a purified specific colony with the disinfected anoectochilus formosanus seeds in the sterile sawdust matrix, culturing for one week under a dark condition, and then culturing at specific illumination intensity and temperature for specific illumination time until the seeds germinate. According to the technical scheme, the germination velocity of the anoectochilus formosanus seeds is increased, meanwhile, the method has good repeatability due to a clear principle.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, and further relates to the germination induction technology of the orchidaceae Aromatica seeds, in particular to a method for promoting the germination and seedling formation of the Aromatica seeds. Background technique [0002] Anoectochilus roxburghii (Wall.) Lindl. belongs to the plant of the genus Anoectochilus of the family Orchidaceae, and is a ground orchid. Clematis not only has high medicinal value, but also has the reputation of "King of Medicine" and "Golden Grass" among the people. It is also an indoor ornamental treasure with great ornamental value. The seeds of golden clematis are underdeveloped and have no endosperm, so it is difficult to germinate independently under natural conditions. In addition, the natural environment has been severely damaged in recent years, birds, insects, snakes, etc. have been harmed, and artificial excessive excavation has brought wild golden ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00A01C1/08C12N1/14C12N1/02
CPCA01C1/00A01C1/08C12N1/02C12N1/14
Inventor 杨柏云罗火林
Owner NANCHANG UNIV
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