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Low temperature preservation method for roxburgh anoectochilus terminal bud germplasm resources

A technology for low-temperature preservation and clematis, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as unreported preservation technology of clematis germplasm resources, and achieve prolonging the time interval of subculture, practical Strong and stable effect

Active Publication Date: 2016-05-11
李操
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, people's research on clematis is limited to rapid reproduction, medicinal ingredients, etc., but there is no report on the preservation technology of clematis germplasm resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for cryopreservation of clematis germplasm resources, comprising the following steps:

[0029] 1) Collection of wild seedlings: collect clematis plants with robust growth and no pests and diseases in the original location;

[0030] 2) Raw material treatment: Clean the collected wild seedlings, dry the water, soak them in 3-5g / L sterilized washing powder liquid for 20-30 minutes, rinse them and then dry the water at a temperature of Passivation at 35-45°C for 10-12 hours;

[0031] 3) Aseptic operation: cut the shoot tips or stem segments of the seedlings, soak the shoot tips or stem segments in 70% ethanol for 1-5 minutes, rinse them once with sterile water, and then soak them in 10% H2O2 After soaking for 5-10 minutes, rinse with sterile water for 2 times, and finally soak with 0.4% HgCL2 for 3-7 minutes, rinse with sterile water for 2 times, cut off the stem tips or stem segments and transfer them to the primary culture of Clematis In the base, MS is the bas...

Embodiment 2

[0038] A method for cryopreservation of clematis germplasm resources, comprising the following steps:

[0039] 1) Collection of wild seedlings: collect clematis plants with robust growth and no pests and diseases in the original location;

[0040] 2) Raw material treatment: Clean the collected wild seedlings, dry them in the air, soak them in 3g / L sterilized washing powder for 30 minutes, rinse them clean and dry them, and blunt them at a temperature of 35°C. 12 hours;

[0041] 3) Aseptic operation: Cut the shoot tips or stem segments of the seedlings, soak the shoot tips or stem segments in 70% ethanol for 1 minute, rinse them once with sterile water, and then soak them in 10% H2O2 for 5 minutes. minutes later, rinsed twice with sterile water, and finally soaked in 0.4% HgCL2 for 3 minutes, rinsed twice with sterile water, cut off the stem tips or stem segments, and transferred the leaves into the first-generation medium of clematis. The first generation medium of clematis ...

Embodiment 3

[0048] A method for cryopreservation of clematis germplasm resources, comprising the following steps:

[0049] 1) Collection of wild seedlings: to collect healthy and pest-free clematis plants in the original situ;

[0050] 2) Raw material processing: clean the collected wild seedlings, dry them, soak them in 5g / L sterilized detergent for 20 minutes, rinse them, dry them, and blunt them at a temperature of 45°C. 10 hours;

[0051] 3) Aseptic operation: cut the stem tip or stem section of the seedling, soak the stem tip or stem section of the seedling in 70% ethanol for 5 minutes, rinse with sterile water once, and then soak in 10% H2O2 for 10 Minutes later, wash 2 times with sterile water, and finally soak 7 minutes with 0.4% HgCL After rinsing 2 times with sterile water, cut off the stem tip or stem segment and leave the leaves and transfer them to the primary culture medium of Clematis clematis. The first-generation medium of Clematis clematis uses MS as the base medium, a...

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Abstract

The invention discloses a low temperature preservation method for roxburgh anoectochilus terminal bud germplasm resources. The low temperature preservation method comprises the following steps: collecting wild seedlings, carrying out primary treatment and high temperature passivation on explants; carrying out germicidal treatment on a clean bench, and then transferring into a roxburgh anoectochilus terminal bud initial medium for low temperature induced differentiation; carrying out low-temperature dark treatment for a period of time after the differentiation, and then transferring bud balls into a roxburgh anoectochilus terminal bud subculture medium; cultivating the bud balls for a period of time under the condition of normal-temperature radiation, then transferring the bud balls for low-temperature dark treatment, and finally transferring plantlets into a roxburgh anoectochilus terminal bud medium for cultivation under normal-temperature radiation. The method disclosed by the invention has the advantages of low cost, simple operation and high practicability, and can protect the precious medicinal resource of roxburgh anoectochilus terminal bud well, so that the biological diversity is protected.

Description

technical field [0001] The invention belongs to the technical field of plant preservation, and in particular relates to a cryopreservation method for clematis germplasm resources. Background technique [0002] Clematis, also known as golden thread orchid and golden thread grass, is a perennial and rare Chinese herbal medicine of the orchid family, the genus Mosaic. It is widely used in the folk, and is known as "the king of medicine", "golden grass", "magic medicine", "black ginseng" and so on. According to the determination of relevant departments, the amino acid composition, composition, content and anti-aging active trace element content in golden silk lily are higher than domestic and wild American ginseng. It has been used as a folk herbal medicine for hundreds of years. Functions and indications: clearing heat and cooling blood, removing dampness and detoxifying, balancing yin and yang, strengthening the body and strengthening the body, complementing yin and yang, pr...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 李操
Owner 李操
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