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Rapid propagation method of dendrobium officinale

A fast technology of Dendrobium candidum, applied in the field of rapid propagation of Dendrobium candidum, can solve the problems of insufficiently developed root system, undeveloped leaves, low yield of fresh strips, etc., and achieves the effects of reducing the use of hormones, ensuring safety, and having dark green leaves.

Inactive Publication Date: 2014-07-30
厦门涌泉科技有限公司
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  • Claims
  • Application Information

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Problems solved by technology

[0004] However, in the prior art, most stems of Dendrobium candidum tissue-cultured seedlings are relatively thin, and most of them are even "toothpick seedlings". Lower output, higher production cost

Method used

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  • Rapid propagation method of dendrobium officinale
  • Rapid propagation method of dendrobium officinale
  • Rapid propagation method of dendrobium officinale

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Embodiment

[0020] The invention discloses a method for rapid propagation of Dendrobium officinale, comprising the following steps:

[0021] 1. Selection of explant material and acquisition of sterile material

[0022] Individuals with rust-colored epidermis, obvious stem nodes, relatively transparent whole body and less fiber were selected as explants. Clean and disinfect the explants first, the specific steps are as follows: first soak for 15 minutes in an aqueous solution of phosphorus-free washing powder, then rinse with running water for 30 minutes; soak in 75% alcohol for 15 seconds, rinse once with sterile water, and then use 0.1% HgCl 2 The solution was sterilized for 8 minutes and washed 5 times with sterile water.

[0023] After the above-mentioned explants are cleaned and disinfected, the water is blotted and cut into 1.0-1.5 cm stem segments, and then inserted into induction medium for induction culture.

[0024] 2. Induction of adventitious buds

[0025] Inoculate the exp...

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Abstract

The invention discloses a rapid propagation method of dendrobium officinale. The rapid propagation mehod comprises the following steps: washing and sterilizing an explant; inoculating the explant in an adventitious bud induction medium which comprises an MS basic culture medium, 2.0mg / L-3.0mg / L of 6-BA (6-Benayl Aminopurine) and 0.2mg / L of NAA (N-Acetyl Aspartate); inoculating an adventitious bud stem in a protocorm induction medium which comprises a B5 basic culture medium, 2.0mg / L of the NAA, 0.2mg / L of the 6-BA and 0.5mg / L-2.0mg / L of KT; inoculating a protocorm in a protocorm propagation culture medium which comprises 1 / 2 of the MS basic culture medium in the step 1, 50g / L of coconut juice; transferring the protocorm in a differential medium which comprises 1 / 2 of the MS basic culture medium in the step 1, 0.2mg / L of the NAA, 80g / L-100g / L of a potato extract; inoculating differentiated seedlings in a seedling and root growing culture medium which comprises 1 / 2 of the MS basic culture medium in the step 1 and 100g / L-150g / L of a banana extract. The rapid propagation method disclosed by the invention has the effects of increasing the survival rate of tissue cultured seedlings of the dendrobium officinale and by using the method, plants of the dendrobium officinale grow healthily and strongly.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a rapid propagation method of dendrobium officinale. Background technique [0002] Dendrobium officinale is a perennial epiphytic herb of Orchidaceae, which grows on semi-shady rocks in mountains at an altitude of 1600 meters. The medicinal ingredients of Dendrobium officinale are mainly Dendrobium polysaccharides, dendrobine and total amino acids, etc., and contain calcium, iron, zinc, selenium, sodium and other trace elements, which have unique medicinal value. Traditional Chinese medicine uses its stems as medicine, named "Dendrobium", which belongs to the yin-tonifying medicine in the tonic. Thin, strong yin, long-term use to thicken the stomach." "Compendium of Materia Medica" evaluates Dendrobium officinale as "strengthening yin and benefiting essence. It thickens the stomach, nourishes the deficiencies in the interior, calms stomach qi, grows muscles, improves intelligence a...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 李璐林江波陈德宗潘菲
Owner 厦门涌泉科技有限公司
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