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Thin layer culture method of tulips

A thin-layer culture and tulip technology, applied in the field of plant tissue culture, can solve problems such as the limitation of the proliferation effect of bulb bulbs, and achieve the effects of efficient and stable genetic transformation system, robust growth, and increased bulb induction number

Inactive Publication Date: 2011-06-15
DASHUN INT FLOWER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Plant tissue culture technology effectively solves this problem. At present, bulbous bulbs such as lilies and tulips are mainly used as explants, which not only require a large number of bulbs, but also greatly limit the proliferation effect.

Method used

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  • Thin layer culture method of tulips
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Examples

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Effect test

example 1

[0011] The experimental group was set as 6 groups, and each group treated 60 explants. Adopt the method of the present invention: the tulip scale cross-cutting (tTCL) becomes thin layer (specific thickness sees Table 1), spreads on the culture medium; Induces the growth medium to add 6-BA per liter, NAA, 2,4- D (see Table 1 for the specific concentration), MS medium with 20 g of sucrose and 6 g of agar; the culture condition is a temperature of 23±2° C.; the light intensity is 1500-2000 Lx, and the light time is 16 h / d.

[0012] The control was set as follows: the whole piece of tulip tissue culture scale was inoculated on the MS medium supplemented with 6-BA 2.0mg / L, NAA 0.2mg / L, 30g / L sucrose and 6g / L agar per liter. The culture conditions are all at a temperature of 23±2°C; the light intensity is 1500-2000Lx, and the light time is 16h / d. 60 explants were processed.

[0013] Table 1 Multi-factor investigation on the effect of seed ball induction on the experimental group a...

example 2

[0017] The source and cutting mode of the scales in the method of the present invention are compared.

[0018] Taking tulip commercial bulbs as the source, the specific treatment method for the scales is: peel off the corky scales on the outer layer of the tulip commercial bulbs, take the healthy scales without disease spots, wash them with running water for 30 minutes, and use 70% of them on the ultra-clean work. Soak in ethanol solution for 30s, then soak in 2% sodium hypochlorite solution for 15min, rinse with sterile water 5 times. Using tulip tissue cultured bulbs as the source, the outer and middle scales are directly removed. Then the scales were cut longitudinally (TCL) or transversely (tTCL) into a thin layer with a thickness of 0.5 mm, and spread on the culture medium; the culture medium for inducing proliferation was supplemented with 6-BA 2.0 mg per liter, NAA 0.5 mg / L, 2 , 4-D 2.0mg, sucrose 20g and agar 6g MS medium. The culture conditions were the same as abov...

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Abstract

The invention relates to the field of flower propagation methods, in particular to the field of corm flower propagation methods. A thin layer culture method of tulips comprises the following steps of: longitudinally or transversely cutting tulip scales into thin layers of 0.1-5mm, and laying the thin layers on a culture medium; and inducing and proliferating the culture medium into an MS (Murashige and Skoog) culture medium, wherein 1.0-2.0mg of 6-BA (Benzyl Aminopurine), 0.1-1.0mg of NAA (Naphthyl Acetic Acid), 1.0-4.0mg of 2, 4-D (2,4-Dichlorophenoxyacetic acid), 20g of cane sugar and 6g of agaragar are added to the MS culture medium per L, the culture condition is that the temperature is 23+ / - 2 DEG C, the illumination intensity is 1,500-2,000Lx, and the illumination time is for 16h / d. The thin layer culture method of the tulips has the advantages and active effects: the tissue culture bulb inductivity reaches 100%, induced tissue culture seedlings grow vigorously, the source of the tissue culture seedlings is not limited by seasons, and the tissue culture scales are the better source of thin layer culture materials. Cutting modes cause different inductions on bulbs, and the differentiation rate and the bulb induction number of the scales which are transversely cut are higher than those of the scales which are longitudinally cut.

Description

technical field [0001] The invention relates to the field of plant tissue culture methods; in particular, the field of bulb flower propagation methods. Background technique [0002] Tulip (Tulipa gesneriana) Liliaceae, tulip genus. Perennial bulbous flowers, with various flower types, rich colors, tall and straight plant types, and excellent ornamental value, are deeply loved by people. Most of the tulip bulbs in my country are imported from the Netherlands, mainly because the natural reproduction speed of tulips is slow, the reproduction coefficient is low, and the bulb virus is easy to accumulate, causing the bulbs to degrade or gradually die. Plant tissue culture technology has effectively solved this problem. At present, bulbous bulbs such as lilies and tulips are mainly used as explants, which not only require a large number of bulbs, but also greatly limit the proliferation effect. [0003] Thin-layer culture is a new type of culture method, mostly using plant leaves...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 杨铁顺
Owner DASHUN INT FLOWER
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