Special high-speed high-density integral cell culture medium for BHK21 cells and method for preparing same

Abstract

The invention discloses a special high-speed high-density integral cell culture medium for BHK21 cells, which is used for culturing the BHK21 cells and comprises European balanced salt, known chemical component lactalbumin hydrolysate and a DMEM (Dulbecco's Modified Eagle Medium) cell culture medium, wherein the components and the contents in the integral cell culture medium are all known. The invention also discloses a method for preparing the special high-speed high-density integral cell culture medium for the BHK21 cells, which comprises the following steps of: firstly, weighing and grouping all raw materials, and respectively and sequentially carrying out mixed milling on the raw materials; and secondly, sequentially carrying out mixed milling on the mixed and milled raw materials. The high-speed high-density integral cell culture medium has the advantages of stable components, longer quality guarantee period and simple preparation process, is easy to control the quality, is easy to storage and can be widely applied to cell culture.

Description

technical field [0001] The present invention relates to a kind of cell culture medium, especially a kind of BHK 21 A high-speed, high-density integrated cell culture medium dedicated to cells and a preparation method thereof belong to the field of biotechnology. Background technique [0002] Today, BHK 21 Cells are the main cell lines used in vaccine production, and are widely used in the production of vaccines such as foot-and-mouth disease. for BHK 21 The medium for cell culture is generally prepared by a three-in-one method. Specifically, hydrolyzed milk protein is formulated with European-style balanced salt and used in combination with cell culture medium such as DMEM. However, the hydrolyzed milk protein used in the culture medium is generally of animal origin, and there is no commercially available fully chemically synthesized product, and the chemical composition of animal milk hydrolyzed protein is not fixed, so the quality of the culture medium cannot be guarant...

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Problems solved by technology

However, the hydrolyzed milk protein used in the culture medium is generally of animal origin, and there is no commercially available fully chemically synthesized product, and the chemical composition of animal milk hydrolyzed protein is not fixed, so the quality of the culture medium cannot be guaranteed, thus making BHK 21 The quality of the vaccine synthesized by cells cannot be guaranteed; moreover, the shelf life of animal-derived hydrolyzed milk protein is generally short, and the storage environment needs to be strictly controlled, otherwise it is easy to deteriorate; at the same time, the three-in-one preparation used in the medium The process of the method is more loaded down with trivial details, and production efficiency is lower

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