Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Haemophilus parasuis culture medium

A technology of Haemophilus suis and culture medium, which is applied in the field of agricultural microorganisms, can solve the problems such as massive proliferation of Haemophilus parasuis, and achieves the effects of enriching nutrients and improving the separation rate.

Inactive Publication Date: 2021-05-25
北京信得威特科技有限公司
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a culture medium for Haemophilus parasuis, which can improve the separation rate of samples contaminated by Haemophilus parasuis, and the result is easy to determine, while solving the problem of massive proliferation of Haemophilus parasuis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Haemophilus parasuis culture medium
  • Haemophilus parasuis culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1) Preparation of basal culture medium

[0036] Take tryptone 15.0g, plant peptone 5.0g, sodium chloride 5.0g, dipotassium hydrogen phosphate 3.0g, glucose 3.0g, add distilled water to 1000mL. Mix evenly and aliquot, sterilize under high pressure (100MPa) at 121°C for 15min, and cool to room temperature.

[0037] 2) Handling of auxiliary materials

[0038] Nicotinamide adenine dinucleotide: Take 0.1g of nicotinamide adenine dinucleotide, dissolve and mix with 100mL deionized water, aliquot and store at -20°C for later use.

[0039] 0.1% phenol red indicator: Take 0.1 g of phenol red, add 2.82 mL of 0.1 mol / L sodium hydroxide solution to dissolve, add distilled water to 100 mL, subpackage, store at room temperature, and set aside.

[0040] Lincomycin: Take 1.0 g of lincomycin, dissolve it in 10 mL of deionized water, aliquot it, and store it at -20°C for later use.

[0041] Horse serum: stock solution, aliquoted, stored at -20°C for later use.

[0042] Mix the prepar...

Embodiment 2

[0046] 1) Preparation of basal culture medium

[0047] Take tryptone 16.0g, plant peptone 3.0g, sodium chloride 4.0g, dipotassium hydrogen phosphate 2.5g, glucose 2.0g, add distilled water to 1000mL. Mix evenly and dispense, sterilize under high pressure (101MPa) at 121°C for 15min, and cool to room temperature.

[0048] 2) Handling of auxiliary materials

[0049] Nicotinamide adenine dinucleotide: Take 0.1g of nicotinamide adenine dinucleotide, dissolve and mix with 100mL deionized water, aliquot and store at -20°C for later use.

[0050] 0.1% phenol red indicator: Take 0.1 g of phenol red, add 2.82 mL of 0.1 mol / L sodium hydroxide solution to dissolve, add distilled water to 100 mL, subpackage, store at room temperature, and set aside.

[0051] Lincomycin: Take 1.0 g of lincomycin, dissolve it in 10 mL of deionized water, aliquot it, and store it at -20°C for later use.

[0052] Horse serum: stock solution, aliquoted, stored at -20°C for later use.

[0053] Mix the prepa...

Embodiment 3

[0057] 1) Preparation of basal culture medium

[0058] Take tryptone 17.0g, plant peptone 4.0g, sodium chloride 5.0g, dipotassium hydrogen phosphate 2.5g, glucose 2.0g, add distilled water to 1000mL. Mix evenly and dispense, sterilize at 121°C, 103.4MPa high pressure (100MPa) for 15min, and cool to room temperature.

[0059] 2) Handling of auxiliary materials

[0060] Nicotinamide adenine dinucleotide: Take 0.1g of nicotinamide adenine dinucleotide, dissolve and mix with 100mL deionized water, aliquot and store at -20°C for later use.

[0061]0.1% phenol red indicator: Take 0.1 g of phenol red, add 2.82 mL of 0.1 mol / L sodium hydroxide solution to dissolve, add distilled water to 100 mL, subpackage, store at room temperature, and set aside.

[0062] Lincomycin: Take 1.0 g of lincomycin, dissolve it in 10 mL of deionized water, aliquot it, and store it at -20°C for later use.

[0063] Horse serum: stock solution, aliquoted, stored at -20°C for later use.

[0064] Mix the pr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a haemophilus parasuis culture medium. The haemophilus parasuis culture medium consists of a basic culture solution and an auxiliary material, the auxiliary material is a mixture of nicotinamide adenine dinucleotide, a phenol red indicator, linkkinin and horse serum; each L of the basic culture solution comprises the following components: 15.0 to 17.0 g of tryptone; 3.0 to 5.0 g of plant peptone; 4.0 to 5.5 g of sodium chloride; 2.0 to 3.5 g of dipotassium phosphate; 2.0 to 3.5 g of glucose; and the balance of water. The haemophilus parasuis culture medium is rich in nutrient components, very suitable for rapid growth of haemophilus parasuis and capable of improving the separation rate of the haemophilus parasuis in a polluted material, phenol red is added into the culture medium to serve as an indicator, on one hand, growth of the haemophilus parasuis cannot be affected, on the other hand, change of acidity and alkalinity can be indicated, in the growth process of the haemophilus parasuis, glucose is fermented to produce acid, alkali in a culture solution can be neutralized, the color of phenol red is changed from red to yellow, and the growth condition of the bacteria can be judged according to the change of the color of the phenol red.

Description

technical field [0001] The invention relates to a culture medium for Haemophilus parasuis, which belongs to the field of agricultural microorganisms. Background technique [0002] Haemophilus parasuis (HPS) is a small Gram-negative bacillus with pleomorphism. The colony shape is raised, smooth, translucent or gray needle-sized colonies, and it is an aerobic or facultative anaerobic bacterium. . HPS has strict nutritional requirements, and its growth strictly requires nicotinamide adenine dinucleotide (NAD+ or factor V). It is difficult to isolate and culture, and the identification steps are cumbersome. [0003] Isolation of Haemophilus parasuis can collect nasal cavity, lung, pericardial effusion, joint fluid, pleural effusion and other disease materials for isolation. Because this bacteria is very delicate, if the aseptic operation cannot be achieved during sampling, it is easy to be covered by contaminated bacteria during cultivation, and the probability of isolation is...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C12Q1/04C12R1/21
CPCC12N1/20C12Q1/045G01N2333/285
Inventor 任国鑫张庆霞范国兵李县斌周振成李恒永冷锋
Owner 北京信得威特科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com