57 results about "Joint fluid" patented technology

A joint aspirate-facilitating device is provided including a flexible main panel that is secured to the joint to be aspirated. An inflatable bladder is secured to the main panel and is placed in contact with the soft tissues overlying and surrounding the joint space. The bladder is used to apply pressure to selected areas of the joint to compress the fluid sacs in the joint. An access opening is formed in the main panel designating the location where joint fluid is to be aspirated. The joint fluid is directed to flow to the area of the joint exposed at the access opening based on the shape of the bladder that compresses surrounding areas of the fluid sacs. A conventional hand pump may pressurize the bladder or the bladder may be filled with a viscous gel material to generate pressure. Multiple access openings may be formed on the main panel designating locations where aspiration is to be conducted. For multiple access openings, plugs may be used to fill those openings where aspiration is not conducted, thereby maximizing joint fluid flow only to the selected location where aspiration is to be conducted. An ultrasound probe may be attached and incorporated into the device to confirm joint fluid displacement into the targeted location prior to need aspiration. A disposable molded sterile dressing may applied to the access opening to reduce the risk of infections. The device can be used on any joint of the body, and the particular shape of the main panel and bladder accommodates maximum joint fluid flow to the point where aspiration is desired.

Disposable microfluidic cartridges, potentiostat reader devices, systems, kits, and methods to determine the concentration of analyte reaction products in a patient's specimen, such as: blood, saliva, cerebrospinal fluid, joint fluid or tears. The microfluidic cartridges are disposable, paper-based strips comprising multiple assay layers, each layer comprising: a drop zone for receiving a patient specimen; a filter to remove contaminates, and / or isolate assay reactants; a hydrophilic microfluidic channel to direct movement of the specimen down the cartridge; a centered reaction chamber comprising impregnated enzymes and reagents to chemically react with the filtered specimen to produce an analyte; and a detector mechanism. Multiple analytes can be produced in parallel (e.g. one per layer), or sequentially along one layer. The detector mechanism utilizes electro-analytic methods to facilitate the reader device in quantifying each analyte, and each analyte concentration is wirelessly transmitted to an electronic computing device for storage in a patient's medical record.

A joint aspirate-facilitating device is provided including a flexible main panel that is secured to the joint to be aspirated. An inflatable bladder is secured to the main panel and is placed in contact with the soft tissues overlying and surrounding the joint space. The bladder is used to apply pressure to selected areas of the joint to compress the fluid sacs in the joint. An access opening is formed in the main panel designating the location where joint fluid is to be aspirated. The joint fluid is directed to flow to the area of the joint exposed at the access opening based on the shape of the bladder that compresses surrounding areas of the fluid sacs. A conventional hand pump may pressurize the bladder or the bladder may be filled with a viscous gel material to generate pressure. Multiple access openings may be formed on the main panel designating locations where aspiration is to be conducted. For multiple access openings, plugs may be used to fill those openings where aspiration is not conducted, thereby maximizing joint fluid flow only to the selected location where aspiration is to be conducted. An ultrasound probe may be attached and incorporated into the device to confirm joint fluid displacement into the targeted location prior to need aspiration. A disposable molded sterile dressing may applied to the access opening to reduce the risk of infections. The device can be used on any joint of the body, and the particular shape of the main panel and bladder accommodates maximum joint fluid flow to the point where aspiration is desired.

A chamber for testing the reactivity of patient fluids to a metallic implant alloy, such as CoCrMo, and a method of determining the reactivity of the patent to the alloy. A sealed mini-electrochemical cell incorporates the alloy surface as well as reference and counter electrodes coupled to a potentiostat for performing electrochemical measurements of the alloy surface while it is in direct contact with freshly harvested human fluids. Freshly obtained body fluids are thus assessed in terms of their bioelectrochemical reactivity with the alloy surface as a measure of the likely reactivity of the patient to the alloy if it were to be implanted into the patient.

The invention relates to a composition for preventing and treating joint soft tissue injury and complications thereof. A complex formulation, which comprises bone collagen, sodium hyaluronate, mangosteen extract, pomegranate extract and vitamin C in specific proportions, is prepared. The composition has an anti-inflammatory effect, since the generation of PGE2 is reduced by lowering the activity of COX2. Meanwhile, the composition accelerates the restoration and generation of cartilage tissue and joint fluid and lowers the degree of joint inflammation.

The invention provides a medicine composition for preventing and treating arthritis. The medicine composition comprises the following components in percentage by weight: 15%-35% of glucosamine hydrochloride, 20%-40% of biological calcium carbonate, 5%-20% of rhizoma drynariae extract, 1%-5% of casein phosphopeptides, 10%-20% of chondroitin sulfate and 10%-30% of epimedium extract. The medicine composition has the advantages of being capable of obviously inhibiting inflammatory cell factors in serum and joint fluid of arthritics patients, relieving the symptoms and signs of the patients, adjusting the immunologic functions of the patients, and effectively preventing and treating diseases such as rheumatism, rheumatoid arthritis, osteoarthritis and gouty arthritis. The invention also provides an application of the medicine composition in the preparation of medicines for preventing and treating diseases such as the rheumatism, the rheumatoid arthritis, the osteoarthritis and the gouty arthritis.

The invention discloses a non-viral nanometer nucleic acid transportation compound used for treating cartilage defect through intra-articular injection which comprises low-molecular chitosan with molecular weight of 5KD-100KDand percentage composition of amino group of 30-70%, and nucleic acid, wherein N / P of the compound is 0.2:1-10:1.The invention degrades high molecular chitosan to low molecular chitosan, increases water solubility of material, reduces viscosity of solution, reduces influence of sour environment of solvent to cell, improves compound size with nanometer, is in favor of performing genetic transmission to cartilago articularis cell, and protects target nucleic acid from neutralizing with synovial fluid and being degrade by nuclease in lysosome. The invention has simple preparation and abroad application scope; can solve engineering roadblock of cartilage repair with no requirement of operation, complex device and bracket material.

The invention discloses an artificial hip joint cuplike cotyle which is at least made up of biological ceramics or vitallium alloy biological materials. The artificial hip joint cuplike cotyle is characterized in that microtexture array surfaces are formed on the area which can be abraded easily at the friction surface of an inner ball of the artificial hip joint cuplike cotyle, namely, a microtexture array is distributed on the edge area of the surface of the inner ball of the artificial hip joint cuplike cotyle and a microtexture array is distributed on the central area of the bottom of the surface of the inner ball of the artificial hip joint cuplike cotyle, so that a series of miniature storage container group structures for synovial fluid or interstitial fluid can be formed. The artificial hip joint cuplike cotyle has good lubrication performance and is capable of reducing the friction effect between a contact interface of the artificial hip joint cuplike cotyle and a contact interface of an artificial thighbone ball and reducing the occurrences of frictions, abrasions and vibrations.

Tissue implants prepared for the repair of tissues, especially avascular tissues such as cartilage. One embodiment presents an electric potential capable of receiving and accumulating desirable factors or molecules from surrounding fluid when exposed to dynamic loading. In another embodiment the implant promotes tissue conduction by retarding, restricting and controlling cellular invasion through use of gradients until competent tissue forms. Further embodiments of the tissue implants may be formed into a multi-phasic device that provides deep tissue mechanical stimulus by conduction of mechanical and fluid forces experienced at the surface of the implant.

The invention relates to a male joint of a fast connector. The male joint of the fast connector comprises a male joint shell, a male joint valve element is movably assembled in a male joint fluid channel of the male joint shell in a guiding mode, a pressure relief channel used for connecting the male joint fluid channel and the outside atmosphere is arranged in the male joint valve element and comprises a liquid passing hole segment connected with the male joint fluid channel and an installing hole segment connected with the outside atmosphere, a plugging piece is movably assembled in the installing hole segment in a guiding mode, a pressure relief spring used for applying elastic acting force to the plugging piece and enabling the plugging piece to plug the liquid passing hole segment is arranged on the male joint valve element, and a guiding channel used for connecting the liquid passing hole segment and the installing hole segment after the plugging piece is separated from the liquid passing hole segment is arranged on the plugging piece and the male joint valve element or between the plugging piece and the male joint valve element. The male joint of the fast connector can perform automatic pressure relief.

The invention discloses a joint fluid drainage irrigator which comprises an irrigation channel and a puncture needle rod inserted in the irrigation channel. An irrigation handle is arranged at the rear end of the irrigation channel; a suction valve is arranged between the irrigation handle and the irrigation channel; and a drainage handle matched with the irrigation handle is arranged at the rear end of the puncture needle rod. The joint fluid drainage irrigator has a novel structure, is convenient to operate and can completely clean the joint fluid in a joint cavity, thereby effectively removing the diseases.

As an FAM19A4 protein is possibly related to various physiological and pathological conditions and plays an important role in the physiological and pathological conditions, and quantitative detectionof the soluble FAM19A4 protein in specific samples such as blood, body fluid and cell culture supernatant has significance. The invention firstly relates to an application of the FAM19A4 protein serving as a diagnosis marker of a disease including an infectious disease and an autoimmune disease. The invention further relates to a method and kit for quantitatively detecting the soluble FAM19A4 protein. According to the method and kit, the soluble FAM19A4 protein is detected through a micro-sphere solid carrier and a flow cytometry technique by the aid of the double-antibody sandwich method principle, and the method and kit can be used for quantitatively detecting the FAM19A4 protein in body fluids such as serum, plasma, urine, pleuroperitoneal fluids, joint fluids, cerebrospinal fluids, amniotic fluids and follicular fluids of a clinic patient, basic research and various biological samples such as cell culture fluids and mouse blood in a mouse model.

This invention relates to: a method for differentiating osteoclast precursor cells (preosteoclasts) into osteoclasts, which comprises culturing the preosteoclasts in the absence of accessory cells; a method for isolating preosteoclasts, which comprises culturing peripheral blood or joint fluid in the absence of cytokine for 1 to 3 weeks; an preosteoclasts, which is obtainable by the above method; a method for differentiating the preosteoclasts obtained by the above method into osteoclasts, which comprises culturing the preosteoclasts in the absence of accessory cell; an osteoclast, which is obtainable by the above method; a method for screening agents for treating metabolic bone diseases, which comprises using the preosteoclasts or the osteoclasts as described above; and agents for treating metabolic bone diseases, which is obtainable by the above screening method.

This invention relates to: a method for differentiating osteoclast precursor cells (preosteoclasts) into osteoclasts, which comprises culturing the preosteoclasts in the absence of accessory cells; a method for isolating preosteoclasts, which comprises culturing peripheral blood or joint fluid in the absence of cytokine for 1 to 3 weeks; an preosteoclasts, which is obtainable by the above method; a method for differentiating the preosteoclasts obtained by the above method into osteoclasts, which comprises culturing the preosteoclasts in the absence of accessory cell; an osteoclast, which is obtainable by the above method; a method for screening agents for treating metabolic bone diseases, which comprises using the preosteoclasts or the osteoclasts as described above; and agents for treating metabolic bone diseases, which is obtainable by the above screening method.

The invention discloses a robot for conservative treatment of gonitis. The robot comprises a U-shaped thigh positioning groove, an ankle clamping mechanism and a periodic stretching mechanism, wherein the thigh of a patient suffering from gonitis is fixed in the thigh positioning groove, the ankle clamping mechanism clamps the ankle of the patient suffering from gonitis, and the periodic stretching mechanism is mounted between the thigh positioning groove and the ankle clamping mechanism, and is used for periodically stretching the legs of the patient and resetting the affected legs by virtue of self elasticity. The leg of a patient is periodically stretched and the affected leg is reset by virtue of self elasticity, so that the stress of the knee joint and the process that joint fluid flows into a joint capsule during repeated leg swinging are simulated, the growth of cartilage in the joint can be effectively promoted, and the treatment effect of knee osteoarthritis is ensured; and the patient does not need to stand in the treatment process, the risks of knee joint abrasion, patient falling and the like can be avoided, and the use requirements of all knee osteoarthritis patients can be met.

The invention discloses a retaining plate. The retaining plate comprises a retaining plate body and is characterized in that a cleaning device is arranged on the surface of the retaining plate body, grooves are arranged in the left side and the right side of the retaining plate body, and the cleaning device can move on the surface of the retaining plate body in a reciprocating manner to clean splattered objects on the surface of the retaining plate body into the grooves. The retaining plate is a splattering-preventing transparent retaining plate used in joint replacement surgery, the surface of the transparent retaining plate adopts a grain surface diffuse reflection design, and the view of a surgeon can be prevented from being affected by high-intensity reflected light under a shadowlesslamp so as to prevent side injury. The retaining plate can well block the splattered objects during use, can fast and repeatedly remove foreign matter such as bone dregs, blood and joint fluid splatted to the retaining plate, can guarantee the view clearness of the surgeon during surgery, can prevent important soft tissue structures from being injured during electric saw operation and can increasethe safety of bone sawing using an electric saw. Meanwhile, the retaining plate can prevent objects from splattering to the eyes and skin of medical staff to cause cross infection so as to well protect the medical staff.

A specific polypeptide related to rheumatoid arthritis and application thereof are provided. The specific polypeptide includes citrulline, wherein at least one side of the citrulline is connected witha citrulline-like amino acid, and a spacer amino acid is also provided between the citrulline and the citrulline-like amino acid. The specific polypeptide provided has high sensitivity and specificity in identifying autoimmune antibodies of patients with rheumatoid arthritis, and can be directly used for the detection of RA autoimmune antibodies, and can also be used to immunize animals to produce polyclonal antibodies or monoclonal antibodies that can be used to detect citrullinated proteins, and to produce identification kits for citrullinated proteins in rheumatoid arthritis serum, joint fluid and body fluids.

The invention discloses application of inhibitory cells derived from a medullary system in preparation of diagnostic reagents for autoimmune diseases. It is found that MDSCs and its subgroup G-MDSCs,M-MDSCs are obviously different in normal people and patients with autoimmune diseases, and only peripheral blood and / or joint fluid sampling is needed, the ratio can be judged by flow cytometry, a specific value is calculated, and the detection is very simple and convenient; in addition, by setting a cut-off value (mean value of normal human + 3SD), higher accuracy can be further ensured, the autoimmune disease can be determined without comprehensive judgment combined with other markers, good indication can be provided for diagnosis and drug treatment effects of the autoimmune disease, and clinical application is facilitated.

The invention discloses a finger-wrist joint rehabilitative apparatus. The finger-wrist joint rehabilitative apparatus comprises a main machine and a limb fixing mechanism connected to the main machine through a connecting plate. The connecting plate comprises a rotary part rotating relative to the main machine and a fixing part fixed relative to the main machine. The limb fixing mechanism comprises a fingerprint fixing mechanism fixed on the rotary part and a wrist fixing mechanism fixed on the fixing part. The main machine comprises a motor assembly used for driving the rotary part to rotate and a control system used for controlling the motor assembly. The finger-wrist joint rehabilitative apparatus has the following beneficial effects: the finger-wrist joint rehabilitative apparatus is simple in structure and convenient to use; joints and wrist joints can passively flex and extend in a repeatedly and continuously manner so that joints are simulated; therefore, a virtuous circle is produced by a physiological joint fluid so that swelling of joints is mitigated; possible phenomena such as reduction in the joint movement range and stiffness of joints due to wounds or insufficient exercises are prevented or treated; and the spasm protection function is obtained so that a patient is protected in case of limb spasticity, thereby preventing the patient from secondary harm.

The invention relates to the technical field of biology, and particularly relates to a preparation method of a cervus and cucumis polypeptide and application of the cervus and cucumis polypeptide to the preparation of special medical-purpose food. According to the cervus and cucumis polypeptide prepared by the preparation method, an extracting solution is subjected to enzymatic hydrolysis with compound proteinase; tannin and an alkaloid are removed with calcium sulfate; the generation of a sensitizer is avoided; meanwhile, the stability of a product is also improved; the polypeptide prepared by the preparation method is ground into powder; the administration can be carried out through an oral taking way; the convenience of the administration is improved. Shown by an experiment, the cervusand cucumis polypeptide prepared by the preparation method can be used for effectively decreasing contents of IL(Interleukin)-1Beta and TNF (Tumor Necrosis Factor)-Alpha in joint fluid of model rats;an obvious difference is presented compared with a control group, and p is less than 0.05.

The invention discloses a biological agent for preventing and relieving joint sub-health and a preparation method thereof, and belongs to the technical field of biological agents. The biological agent of the present invention contains a plurality of bioactive components and a plurality of active ingredients of Chinese herbs. The bioactive components comprise S-adenosylmethionine, sodium hyaluronate, sodium chondroitin sulfate, vitrin and acetyl glucosamine, can prevent decomposition of proteoglycan by enzyme, provide exogenous hyaluronic acid for human joints, activate synthesis of mucopolysaccharide in the body, and promote synthesis of hyaluronic acid and collagen and cartilage regeneration. The bioactive components can improve content of hyaluronic acid in the body, reduce cartilage loss and maintain morphology of cartilage and synovial fluid. The active ingredients of Chinese herbs comprise fructus liquidambaris extract, herba lycopodii extract, fructus morinda citrifolia and ginger root extract, and have the effects of relaxing tendons and activating collaterals, promoting blood circulation to remove blood stasis, improving local microcirculation and relieving pain. According to the biological agent, Chinese medicines and western medicines are combined to fundamentally prevent and relieve joint inflammation.

The invention discloses joint cavity injection for treating osteonecrosis, and belongs to the field of biomedicine. Sodium hyaluronate, graphene quantum dots and BMP (bone morphogenetic protein) 2 / 7 heterodimers are mixed with one another according to certain quantities to obtain the joint cavity injection. The joint cavity injection has the advantages that the joint cavity injection can directlyreach affected parts when administrated by means of injection, accordingly, pathological joint fluid can be improved, lubrication functions can be increased, the osteogenic activity of mesenchymal stem cells can be excited, osteogenesis of osteoblasts can be promoted, osteolysis of osteoclasts can be inhibited, osteonecrosis repair can be promoted, and functions of joints can be reserved; the joint cavity injection is used for treating osteonecrosis related diseases related to the joints.

The invention discloses a preparation containing human joint fluid exosome. The preparation comprises 20-30% of the human joint fluid exosome, 15-18% of a polyethylene glycol solution, 7-12% of humanalbumin, 6.5-7.5% of methionine, 5.3-6.7% of propylene carbonate, 2.1-3.8% of vitamin C, 1.35-2.28% of tranexamic acid, 4.87-5.86% of a solvent, 5.66-7.32% of a phosphatidyl sarcosine complex, and thebalance of deionized water. The invention further discloses a using method of the preparation containing the human joint fluid exosome. The using method includes the following steps: separation and extraction of the human joint fluid exosome, production of the preparation, sterilization, resuscitation cryopreservation and injection use. The above formula design is reasonable, and the human jointfluid exosome is obtained by separation and extraction, and has a good treatment effect on joint diseases, so the exosome preparation is more safe than drug or surgical treatment, and reduces the trauma to the patient's body; and the exosome preparation can be preserved for a long time after being produced, is very convenient to use, has a good treatment effect, and facilitates promotion and application.

The invention discloses a fluid centrifugal control system. The system comprises a tee-joint fluid channel body and a structural body. The tee-joint fluid channel body comprises an inflow channel, an outflow channel and a discharging channel. The structural body corresponds to the discharging channel. Rotating centrifugal force and pressure of fluid in the inflow channel jointly act on the structural body. The included angle between the acting direction of the fluid, acting on the structural body, in the inflow channel and the acting direction of the rotating centrifugal force acting on the same structural body is larger than 90 degrees. The displacement of the structural body controls the circulating state of the discharging channel. The fluid centrifugal control system has the beneficial effects of being simple in structure, reliable in performance and the like.

The invention discloses an autologous joint fluid source mesenchymal stem cell repair cartilage injury image segmentation method based on multi-modal magnetic resonance. The method comprises the following steps of S1, collecting a cartilage injury image, S2, carrying out image visualization processing, S3, carrying out image edge detection, S4, extracting characteristic parameters, S5, extractingseed points, S6, selecting non-seed points, S7, updating the regional gray average value and the pixel standard deviation K of all the seed points in a growth region, and S8, finishing image acquisition. According to the method, 15-20 characteristic parameters are extracted from each edge; wherein the information features comprise edge local information features, edge geometric information features and information features between edge adjacent voxels, the features of different edge lines can be fully embodied, the classification screening precision is improved, and the method realizes intelligent selected image segmentation through self-adaptive seed point extraction and algorithm screening, and improves the image processing speed.

The invention discloses an ultra-sensitive electrochemical LCR sensor for detecting methicillin-resistant staphylococcus aureus in synovial fluid. The ultra-sensitive electrochemical LCR sensor comprises the following steps of (1) designing two specific double-stranded short probes (S-dsDNA) according to conservative series of a MecA gene; (2) in an LCR reaction system, taking the MecA gene as a template, connecting two short series of probes through DNA ligase to form long double-stranded DNA (L-dsDNA), and then carrying out cyclic amplification by using an L-dsDNA template to form a large amount of L-dsDNA, and (3) fixing the formed L-dsDNA with sulfydryl and biotin modification on a BSA modified gold electrode through a gold-sulfur bond, dropwise adding avidin-PolyHRP to be combined with biotin on the L-dsDNA, finally putting the electrode into a base solution containing TMB and H2O2, and enabling H2O2 to oxidize TMB to generate an electrochemical signal under the catalysis of HRP. The sensor has the advantages of economy, quickness, high sensitivity, specificity and the like, can be used for detecting a single base mutation series, and realizes the detection of the MRSA in the synovial fluid of a patient infected around the joint prosthesis.

The invention provides a shield excavation face spread joint fluid-solid coupling safety degree analysis method which comprises the steps that a spread joint strain softening model of an excavation face is established, a rock mass of the excavation face is divided into a plurality of units, and each unit is composed of a rock block and a joint; judging an elastic stage and a yield stage of the rock mass and the joint; safety calculation is conducted on the rock blocks and joints in the elastic stage / yield stage; selecting the rock mass / joint with the minimum safety degree in the elastic stage / yield stage; permeability coefficient calculation is conducted on the rock mass / joint with the minimum safety degree; substituting the safety coefficient into fluid-solid coupling numerical calculation to carry out iterative calculation so as to monitor the dynamic change of the permeability coefficient; by calculating the safety degree of each unit of the rock mass and determining the permeability coefficient of the rock mass, the shield excavation surface local stability monitoring of rock mass fluid-solid coupling is realized, the permeability coefficient dynamic change of the rock mass spread over joint characteristics is clearly reflected, and the shield excavation surface analysis accuracy under the spread over joint condition is improved.