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Preparation method of cervus and cucumis polypeptide and application of cervus and cucumis polypeptide to preparation of special medical-purpose food

A technology of deer melon polypeptide and deer bone, which is applied to the preparation of deer melon polypeptide, and the application field of deer melon polypeptide in the preparation of food for special medical purposes, which can solve problems such as allergic reactions and loss of polypeptide functions

Active Publication Date: 2018-05-04
JILIN JINUO BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tannins, alkaloids and proteins can react to form precipitates that are insoluble in water and difficult to digest and absorb. Tannins and alkaloids can also combine to form precipitates that are insoluble in water and difficult to digest and absorb, resulting in allergic reactions and loss of polypeptide functions.

Method used

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  • Preparation method of cervus and cucumis polypeptide and application of cervus and cucumis polypeptide to preparation of special medical-purpose food
  • Preparation method of cervus and cucumis polypeptide and application of cervus and cucumis polypeptide to preparation of special medical-purpose food

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preparation example Construction

[0083] The present invention provides a preparation method of velvet polypeptide and the application of velvet polypeptide in the preparation of food for special medical purposes. Those skilled in the art can learn from the content of this article and appropriately improve the process parameters to realize it. In particular, it should be pointed out that all similar replacements and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The method and application of the present invention have been described through preferred embodiments, and relevant personnel can obviously make changes or appropriate changes and combinations to the method and application herein without departing from the content, spirit and scope of the present invention to realize and apply the present invention Invent technology.

[0084] The test materials used in the present invention are all common commercially available products, which...

Embodiment 1

[0087] (A) Preparation of deer bone polypeptide extract:

[0088] (1) Clean fresh deer bones, crush the deer bones, add water for injection, and extract 3 to 5 times under the conditions of 85°C and 0.10-0.3 MPa;

[0089] (2) After combining the above extracts, adjust the pH value to 4.0-5.0, then let it stand at 4-7°C for 15 hours, then filter it with a ceramic membrane with a pore size of 50 microns, and centrifuge the filtrate with a centrifuge at 1500 rpm for 20 minutes;

[0090] (3) After collecting the supernatant, adjust the pH value to 8-9, then let it stand at 4-8°C for 15 hours, then filter it with a ceramic membrane with a pore size of 25 microns, centrifuge the filtrate with a centrifuge at 1600 rpm for 20 minutes, and collect the Clear liquid obtains deer bone crude extract;

[0091] (4) Kjeldahl method is used to measure the content of protein and polypeptide in the crude extract of deer bone, and the pH value of the crude extract of deer bone is adjusted to be ...

Embodiment 2

[0106] (A) Preparation of deer bone polypeptide extract:

[0107] (1) Cleaning fresh deer bones, crushing the deer bones, adding water for injection and extracting 3 to 5 times under the conditions of 95°C and 0.15-0.4 MPa;

[0108] (2) After merging the above extracts, adjust the pH value to 4.5-5.5, then let it stand at 6-8°C for 18 hours, then filter it with a ceramic membrane with a pore size of 50 microns, and centrifuge the filtrate with a centrifuge at 1800 rpm for 20 minutes;

[0109](3) After collecting the supernatant, adjust the pH value to 7-8, then let it stand at 4-7°C for 18 hours, then filter it with a ceramic membrane with a pore size of 25 microns, centrifuge the filtrate with a centrifuge at 1800 rpm for 20 minutes, and collect the above Clear liquid obtains deer bone crude extract;

[0110] (4) Kjeldahl method is used to measure the content of protein and polypeptide in the crude extract of deer bone, adjust the pH value of the crude extract of deer bone t...

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Abstract

The invention relates to the technical field of biology, and particularly relates to a preparation method of a cervus and cucumis polypeptide and application of the cervus and cucumis polypeptide to the preparation of special medical-purpose food. According to the cervus and cucumis polypeptide prepared by the preparation method, an extracting solution is subjected to enzymatic hydrolysis with compound proteinase; tannin and an alkaloid are removed with calcium sulfate; the generation of a sensitizer is avoided; meanwhile, the stability of a product is also improved; the polypeptide prepared by the preparation method is ground into powder; the administration can be carried out through an oral taking way; the convenience of the administration is improved. Shown by an experiment, the cervusand cucumis polypeptide prepared by the preparation method can be used for effectively decreasing contents of IL(Interleukin)-1Beta and TNF (Tumor Necrosis Factor)-Alpha in joint fluid of model rats;an obvious difference is presented compared with a control group, and p is less than 0.05.

Description

technical field [0001] The present invention relates to the field of biotechnology, in particular to a method for preparing a deer melon polypeptide and the application of the deer melon polypeptide in preparing food for special medical purposes. Background technique [0002] Cervus melon polypeptide is a product made by extracting the bones of the cervus sika deer (Cervus Nippon Temmick) and the dried and mature seeds of the cucurbit plant muskmelon (Cucumis melo L.). It is rich in various free amino acids. Osteocytes synthesize BMPs, TGF-β, FGF and other bone-derived biological factors to provide raw materials and promote the synthesis of bone-derived biological factors. Organic calcium and phosphorus ions can participate in calcium and phosphorus metabolism and maintain bone capacity. Clinically, Lugua Polypeptide is an injection suitable for rheumatism, rheumatoid arthritis, early healing of fractures, osteoarthritis, waist and leg pain, and trauma recovery. It is a lig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34A61K38/01A61P19/02A61P29/00A61P19/08
CPCA61K38/012C07K1/34C12P21/06
Inventor 王雷潘风光王爽
Owner JILIN JINUO BIOLOGICAL ENG CO LTD
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