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Equine embryo-flushing solution as well as preparation method and applications of solution

A preparation method and egg flushing technology, which are applied in the field of animal embryo transplantation, can solve the problems of difficult drug sources, complicated preparation steps, need to be used and prepared now, and achieve the effects of being favorable for popularization and application, simple preparation and convenient material sampling.

Active Publication Date: 2013-01-09
QINGDAO DERUI JUNFA BIOLOGY TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the problems of difficult source of drugs, high price, cumbersome preparation steps, and the need for immediate use and preparation of traditional egg-washing liquid, the present invention provides an egg-washing liquid for non-surgical embryo transfer of equine animals suitable for large-scale production Its preparation method and application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A liquid for washing eggs of equine animals, comprising 5000ml of sodium lactate mixed solution as the main solution, and every 1000ml of sodium lactate mixed solution contains 500,000 units of penicillin, 500,000 units of streptomycin and 10% by volume of calf serum.

[0030] Weigh 27g of sodium lactate, 16g of sodium chloride, 23g of potassium chloride, 5.5g of calcium chloride and 1.6g of magnesium chloride, add 5000ml of distilled water to mix, place in a thermostat at 37°C for 6 hours, and set aside; weigh 0.4g of astragalus polysaccharide Put it into a sterile beaker, add it to the spare mixed solution into the sterile beaker, stir to fully dissolve the astragalus polysaccharide, and obtain a sodium lactate mixed solution; before preparing eggs, add penicillin, Streptomycin and calf serum can be used to obtain egg flushing solution.

[0031] Use the ovum washing solution of this embodiment to wash eggs for non-surgical bovine embryo transfer.

[0032]

Embodiment 2

[0034] A solution for washing eggs of equine animals, including 5000ml of sodium lactate mixed solution as the main solution, and every 1000ml of sodium lactate Ringer injection contains 1 million units of penicillin, 1.2 million units of streptomycin and 3% inactivated horse serum by volume.

[0035] Weigh 10.5g of sodium lactate, 6g of sodium chloride, 13.5g of potassium chloride and 9g of calcium chloride, add 5000ml of distilled water to mix, place in a thermostat at 37°C for 3 hours, and set aside; weigh 1.4g of astragalus polysaccharide into Add it to the sterile beaker, add it to the spare mixed solution, stir to fully dissolve the astragalus polysaccharide, and get the sodium lactate mixed solution; before preparing eggs, add penicillin and streptomycin to the sodium lactate mixed solution according to the component content And inactivated horse serum, you can get the egg flushing solution.

[0036] The ovum flushing liquid of this embodiment is applied to the ovum flu...

Embodiment 3

[0039] A liquid for washing eggs of equine animals, including 4000ml of sodium lactate mixture as the main solution, wherein every 1000ml of sodium lactate mixture contains 800,000 units of penicillin, 800,000 units of streptomycin and 5% inactivated horse serum by volume; wherein , the content of each component in the sodium lactate mixed solution is: contain sodium lactate 26g, sodium chloride 10.8g, potassium chloride 21g and calcium chloride 16.8g in every 1000ml sodium lactate mixed solution.

[0040] Weigh 26g of sodium lactate, 10.8g of sodium chloride, 21g of potassium chloride and 16.8g of calcium chloride, add 4000ml of distilled water to mix, and place in a thermostat at 37°C for 1 hour to obtain a sodium lactate mixture for use; prepare eggs Before adding penicillin, streptomycin and inactivated horse serum to the sodium lactate mixture according to the content of the components, the egg flushing solution can be obtained.

[0041] The ovum flushing liquid of this e...

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PUM

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Abstract

The invention discloses an equine embryo-flushing solution as well as a preparation method and applications of the solution. The embryo-flushing solution contains sodium lactate mixed liquor, penicillin, streptomycin and one, two or three of bovine serum albumin, neonatal bovine serum and inactivated horse serum. The preparation method comprises the following steps: placing the sodium lactate mixed liquor in thermostat with the temperature of 37 DEG C for 0.5-6 hours for standby; and adding the penicillin, streptomycin and one, two or three of bovine serum albumin, neonatal bovine serum and inactivated horse serum in the sodium lactate mixed liquor according to the component contents to obtain the embryo-flushing solution. The preparation method is suitable for the non-surgical embryo transfer technology of horses, cattle and donkeys. The equine embryo-flushing solution has the following beneficial effects that the solution has the advantages that raw materials are available, cost is low, preparation method is simple and use is convenient, is very suitable for the equine large-scale non-surgical embryo transfer and is beneficial to the popularization and application of the embryo transfer technology.

Description

[0001] technical field [0002] The invention belongs to the field of animal embryo transplantation, and in particular relates to an egg washing liquid for equine animals and its preparation method and application. [0003] Background technique [0004] Embryo transfer, also known as fertilized egg transfer, commonly known as artificial insemination or gestation, refers to the technology of transplanting the early embryos of female animals into other female animals of the same species and the same physiological state, so that they can continue to develop into new individuals. The basic procedures of embryo transfer include donor superovulation and insemination, recipient estrus synchronization, egg collection, egg inspection and transplantation. [0005] Egg collection, also known as embryo recovery, refers to the method of collecting embryos from donors, which can be divided into surgical and non-surgical methods. The non-surgical method is simpler than the surgical metho...

Claims

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Application Information

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IPC IPC(8): C12N5/075A61D19/04
Inventor 荣美洁邹志钢张志鹏李成修于国纲
Owner QINGDAO DERUI JUNFA BIOLOGY TECH
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