95 results about "Embryo transfer (procedure)" patented technology

We describe a method for interrogating the content and primary structure of DNA by microarray analyses and to provide comprehensive genetic screening and diagnostics prior to embryo transfer within an IVF setting. We will accomplish this by the following claims: 1) an optimized embryo grading system, 2) a less invasive embryo biopsy with reduced cellular contamination, 3) an optimized DNA amplification protocol for single cells, 4) identify aneuploidy and structural chromosome abnormalities using microarrays, 5) identifying sub-telomeric chromosome rearrangements, 6) a modified DNA fingerprinting protocol, 7) determine imprinting and epigenetic changes in developing embryos, 8) performing genome-wide scans to clarify / diagnose multi-factorial genetic disease and to determine genotype / haplotype patterns that may predict future disease, 9) determining single gene disorders with or without a known DNA mutation, 10) determining mtDNA mutations and / or the combination of mtDNA and genomic (nuclear) DNA aberrations that cause genetic disease.

An intravaginal fertilization and culture container includes a main chamber and a microchamber. A culture medium, one or more oocytes and sperm are introduced through an access opening having an associated resealable closure device. The container is enveloped in a capsule of soft elastic material prior to lodgment in the posterior fornix. A loop on one capsule part adapts the length to anatomic variations. The microchamber has a channel which receives the catheter tip to facilitate embryo retrieval while eliminating risk of injury thereto. The microchamber permits embryos to be microscopically inspected in situ before transfer to the uterine cavity.

An embryo transfer catheter with a protective sheath is disclosed. The use of a protective sheath shields a guide catheter from cervical mucus. The retracted open flaps of the outer protective sheath draw the mucus and blood away from the inner guide catheter. This allows an inner embryo transfer sheath to enter the uterus unobstructed. The embryo is then transferred to the uterus by means of fluid pressure with a culture medium. The protective sheath may be snap fit onto the guide catheter. The sheaths and catheters may be manufactured in a variety of lengths for individual convenience and then snap-fit onto each other for better control during an embryo implantation procedure. Other catheters and related instruments may also be manufactured with snap-on features.

A speculum is equipped with an ultrasound probe, for permitting ultrasound visualization during procedures, such as transcervical embryo transfer with In Vitro Fertilization, thus providing superior transvaginal ultrasonographic imaging without interference by the ultrasound probe with usual embryo transfer techniques, and without the need for an additional assistant. The probe may be disposed on either of the posterior blade and the anterior blade, depending upon physiological factors varying with each patient and procedure.

An embryo-transfer catheter has an extruded shaft with a gas-filled lumen that extends helically along the catheter to provide an ultrasound marker. The catheter has a side opening close to a closed rounded patient end. The catheter could be completely transparent to x-rays or the plastics from which it is formed could incorporate an x-ray opaque filler, such as barium sulphate.

The invention relates to an apparatus and a method useful for non-surgical embryo transfer or artifical insemination of mammals. An apparatus for depositing media into the uterus of a mammal includes a conical chamber that has a plurality of perforations and flaps. An exterior spiral formation is configured for traversing or penetrating cervical passageway. A sheath having a frusto-conical rearward end extends axially from an aft end of the conical chamber. A tubular depositing chamber extends axially from the conical chamber to a position beyond a fore end of the conical chamber. Coupled to the rearward end of the depositing chamber is an embryo or semen packaging unit. The tubular depositing chamber has an end that has an aperture to permit the flow of semen out of the depositing chamber and into the uterus. The invention also involves a method for depositing of embryos or semen into a uterus of a mammal by a) inserting a conical chamber having a fore end and an exterior spiral formation into a cervix of a mammal, b) securing the conical chamber with the walls of the cervix, c) projecting a depositing chamber through an interior portion of the conical chamber, d) moving the depositing chamber to an embryo or semen release position for release of embryos or semen in the uterus, and e) securing an embryo or semen packaging unit to the depositing chamber to deposit embryos or semen in the uterus.

Crystalline (3Z,5S)-5-(hydroxymethyl)-1-[(2′-methyl-1,1-biphenyl-4-yl) carbonyl]pyrrolidin-3-one O-methyloxime is disclosed. A pharmaceutical composition containing the crystalline compound and methods for treating conditions related to the OT-R activity, such as preterm labor, and tor increasing embryo implantation rate in a mammal undergoing embryo transfer, comprising administering the crystalline compound are also disclosed.

An embryo transfer catheter with a protective sheath is disclosed. The use of a protective sheath shields a guide catheter from cervical mucus. The retracted open flaps of the outer protective sheath draw the mucus and blood away from the inner guide catheter. This allows an inner embryo transfer sheath to enter the uterus unobstructed. The embryo is then transferred to the uterus by means of fluid pressure with a culture medium. The protective sheath may be snap fit onto the guide catheter. The sheaths and catheters may be manufactured in a variety of lengths for individual convenience and then snap-fit onto each other for better control during an embryo implantation procedure. Other catheters and related instruments may also be manufactured with snap-on features.

The methods provided herein relate to swine production. Specifically the instant invention provides methods for the production of terminal parent animals (i.e. terminal sires and terminal dams) for use in swine production herds. Also provided are methods for quickly and efficiently introducing and / or fixing one or more desirable traits or alleles in a swine herd. Alternatively, the present methods may be employed to eliminate a particular undesirable trait or gene. The invention also provides for herds that have been developed using any of the methods described herein. The invention also provides for the use of embryo transfer, including markerassisted embryo transfer to facilitate the transfer of genetic material, particularly when it is desirable to maintain the specific-pathogen free status of a herd.

Instead of immersing human reproductive cells in a single culture medium throughout the various procedures used in IVF, a process is provided by which the reproductive cells may be moved through a sequence of distinct culture media as the various IVF procedures are carried out. In one implementation, the culture media specifically formulated to provide a physical environment similar to that found within the female reproductive tract and conducive to growth and development of human reproductive cells during the various stages of the IVF process. In this regard, specifically formulated culture media can be applied to support the reproductive cells in one or more of the following procedures: oocyte retrieval and handling; oocyte maturation; ordinary fertilization; oocyte, zygote and embryo examination and biopsy; embryonic development to the eight-cell stage; embryonic development to the blastocyst stage; embryo transfer; and cryopreservation.

The invention discloses an embryo pregnancy result prediction device based on multimodality, and belongs to the field of medical artificial intelligence. Firstly, images of embryos developed to blastocyst stage after in vitro fertilization and corresponding pregnancy results are obtained, three pictures of blastocyst, inner cell mass and trophoblast cells of embryos are obtained, and the pregnancyresults are labeled as labels, and data are labeled as raw data. Then the image is smoothed with Gaussian kernel function to remove part of the noise, and then the image is normalized. The image is then data-augmented as input data. Multimodal method is used to fuse the images of the three images so that the input image contains three evaluation features. the fused image is passed to ResNet-50 for training, that network is optimized according to the target tag, and and is iterated until the training is complete. With the model, three images can be taken before embryo transfer to predict the pregnancy outcome, and the embryo with high success rate can be selected according to the output results, which can improve the final pregnancy success rate.

An embryo transfer catheter or other medical device has a shaft extruded with two layers. The outer layer is relatively thick and contains gas bubbles sufficient to increase the visibility of the catheter under ultrasound observation but with a density that allows material within the catheter to be viewed by the eye. The inner layer is relatively thin and is free of bubbles so that it provides a smooth bore to the catheter.

The present invention relates to the use of an oxytocin receptor antagonist in females undergoing embryo transfer as part of an assisted reproductive technology. In particular, methods are provided for increasing ongoing implantation rate, increasing ongoing pregnancy rate, increasing clinical pregnancy rate, and / or increasing live birth rate in a female subject undergoing embryo transfer. Specifically, the antagonists are released in the luteal phase when the endometrium is receptive for embryo implantation and / or when the embryo has reached the blastocyst-stage.

The present invention relates to the use of an oxytocin receptor antagonist in females undergoing embryo transfer as part of an assisted reproductive technology. In particular, methods are provided for increasing ongoing implantation rate, increasing ongoing pregnancy rate, increasing clinical pregnancy rate, and / or increasing live birth rate in a female subject undergoing embryo transfer. Specifically, the antagonists are released in the luteal phase when the endometrium is receptive for embryo implantation and / or when the embryo has reached the blastocyst-stage.

The invention relates to an apparatus and a method useful for non-surgical embryo transfer or artificial insemination of mammals. An apparatus for depositing media into the uterus of a mammal includes a conical chamber that has a plurality of perforations and flaps. An exterior spiral formation is configured for traversing or penetrating cervical passageway. A sheath having a frusto-conical rearward end extends axially from an aft end of the conical chamber. A tubular depositing chamber extends axially from the conical chamber to a position beyond a fore end of the conical chamber. Coupled to the rearward end of the depositing chamber is an embryo or semen packaging unit. The tubular depositing chamber has an end that has an aperture to permit the flow of semen out of the depositing chamber and into the uterus. The invention also involves a method employing the apparatus described.

The present invention provides a premature ovulation inhibitor for use in in vitro fertilization or embryo transfer process, which contains a nonpeptidic compound having a gonadotropin releasing hormone antagonistic action. The premature ovulation inhibitor for use in in vitro fertilization or embryo transfer process of the present invention is low toxic, permits oral administration, and has a superior inhibitory effect on premature ovulation in in vitro fertilization or embryo transfer process.

A method of embryo transfer (“ET”) that improves fertility rates by eliminating transferred air during the procedure is provided. Also provided is a method for hormonally enhancing the uterine wall of a patient either prior to or during the time of ET. Quantitative administration of transfer solutions is accomplished with a modified apparatus that provides for implantation of an embryo into the uterus of a patient. The apparatus comprises an outer sheath and an inner lumen arranged to be slidably disposed within the outer sheath. The inner lumen includes at least one visual marker situated on the exterior surface adjacent its distal end thereof.

The invention discloses a method and device for transporting embryos, and the method and device provided by the invention are applied to an embryo transfer process. In the invention, an insulation container is utilized as a means of delivery, warm water is stored in the container and a light buoy is placed in the warm water, namely, the buoy is borne by means of the warm water so that the embryosloaded in the container are in an environment of an imitative parent substance with uniform and appropriate temperature and slight oscillation. Therefore, the method and the device have the beneficial effects of maintaining the embryonic activity, facilitating the embryonic development and being easy for material selection and convenient for operation during the embryo transfer process.

The invention discloses an effect study method of melatonin in prediction of ovarian reserve and IVF-ET (in-vitro fertilization and embryo transfer) outcome. The method includes: collecting basic clinical data of 61 females, and dividing the 61 females into three groups, namely a poor ovarian response group, a normal ovarian response group and a high ovarian response group mainly through age, retrieved oocyte number and anti-mullerian hormone value according to the stimulus response of the ovaries; collecting follicular fluid of the 61 females, adopting melatonin radioimmunoassay to determine melatonin concentration in the follicular fluid of the 61 females, and analyzing the levels of the melatonin concentration and other clinical data in the poor ovarian response group, the normal ovarian response group and the high ovarian response group; analyzing the levels of relevant data of in-vitro fertilization outcome in the poor ovarian response group, the normal ovarian response group and the high ovarian response group, and verifying relevance of the melatonin concentration and IVF-ET outcome; verifying relevance of the melatonin level and ovarian reserve. Through detection of melatonin concentration in the follicular fluid, ovarian reserve and IVF-ET outcome are predicted.

The present provides novel systems and methods to facilitate embryo transfer, to maximize implantation rates and to improve pregnancy rates resulting from In Vitro fertilization.

A safe recording and comparison method for artificial fertilization, in-vitro fertilization and embryo transfer comprises the following steps of: step 1, archiving of a male parent and a female parent: storing identity information, pairing information and biological feature information; step 2, semen collection: identifying male parent information at first, then collecting semen, and establishing a matching relation between the semen and the male parent; step 3, semen treatment: during treatment, checking whether the semen is matched with the male parent in each sample transfer or transmitting the matching relation; step 4, ovum collection: identifying female parent information at first, then collecting an ovum, and establishing a matching relation between the ovum and the female parent; step 5, ovum fertilization: identifying semen and ovum information at first, then checking whether pairing is allowed, and if so, continuing, or otherwise, sending out an error prompt; and step 6, transplantation: verifying the female parent information at first, then checking whether an embryo is matched with the female parent, and if so, continuing, or otherwise, sending out the error prompt. The checking is performed before every operation to prevent errors.

The invention aims to provides an embryo transfer device and an application method thereof. By the embryo transfer device and the application method thereof, the embryo can be transferred to a correct position in uterus accurately under ultrasonic guidance so that embryo transfer success rate is increased. The embryo transfer device comprises a hard supporting core, a transfer tube and a guide tube, both the transfer tube and the guide tube are through, and an echo steel coil is arranged at the front end of the guide tube. The application method of the embryo transfer device includes steps of A, placing the supporting core into the guide tube, inserting the guide tube into the uterus, observing the position of the echo steel ring in the guide tube by an ultrasonic unit, regulating the guide tube until the guide tube locates at a proper position of the transferred embryo, and then removing the supporting core; B, connecting an injector with the transfer tube carrying the to-be-transferred embryo, inserting the transfer tube into the guide tube, regulating the position of the transfer tube relative to the guide tube until the transfer tube locates at the proper transfer position; and C, utilizing the injector to carry out implanting of the embryo, and then removing the guide tube and the transfer tube so as to complete the embryo transfer process.

The invention discloses an equine embryo-flushing solution as well as a preparation method and applications of the solution. The embryo-flushing solution contains sodium lactate mixed liquor, penicillin, streptomycin and one, two or three of bovine serum albumin, neonatal bovine serum and inactivated horse serum. The preparation method comprises the following steps: placing the sodium lactate mixed liquor in thermostat with the temperature of 37 DEG C for 0.5-6 hours for standby; and adding the penicillin, streptomycin and one, two or three of bovine serum albumin, neonatal bovine serum and inactivated horse serum in the sodium lactate mixed liquor according to the component contents to obtain the embryo-flushing solution. The preparation method is suitable for the non-surgical embryo transfer technology of horses, cattle and donkeys. The equine embryo-flushing solution has the following beneficial effects that the solution has the advantages that raw materials are available, cost is low, preparation method is simple and use is convenient, is very suitable for the equine large-scale non-surgical embryo transfer and is beneficial to the popularization and application of the embryo transfer technology.

Crystalline (3Z,5S)-5-(hydroxymethyl)-1-[(2′-methyl-1,1-biphenyl-4-yl)carbonyl]pyrrolidin-3-one O-methyloxime is disclosed. A pharmaceutical composition containing the crystalline compound and methods for treating conditions related to the OT-R activity, such as preterm labor, and tor increasing embryo implantation rate in a mammal undergoing embryo transfer, comprising administering the crystalline compound are also disclosed.

The invention discloses an embryo transferring model and a teaching method thereof. According to the proportion of a normal female reproductive system, the lengths, widths, thicknesses, volumes and diameters of all parts (the uterine body cavity, cervix and vagina) of a model are specifically quantitatively designed, combined with the lengths of transferring internal and external catheters, an embryo experimentalist and a clinicist can more intuitively and more accurately understand the relative distance and space of a transferring path, thus the embryo transferring position is better controlled, the clinicist can fully feel certain key difficulties in the transferring path, through repeated practice, the difficulties are overcome in formal transferring operation, and smooth intubation isachieved.

The invention discloses a method for improving the test-tube baby clinical outcome by selecting an embryo with good development potential. The method comprises five steps that the transplanting embryo with good development potential is selected mainly according to IL-8 in an embryo culture solution before the transplanting; concretely, a liquid phase chip high-flux protein analysis technology is used for detecting the content of IL-8 in a sample; an IL-8 positive embryo in the culture solution is selected to be combined with a morphology evaluation method to perform transplantation; the IL-8 positive embryo in the culture solution before the transplantation is used for being combined with the morphological high-quality embryo to perform transplantation; the pregnancy rate can be improved by more than 8 percent, and can reach 60.76 percent; compared with the existing transplantation method for selecting the embryo singly by a morphology method, the method provided by the invention has the advantages that the pregnancy rate can be improved by more than 8 percent to 12 percent. When the method is combined with the existing morphology method for selecting high-quality embryo; or the high-quality embryo and the ordinary embryo are transplanted to an in vitro fertilization person, the embryo planting rate can also be improved; the baby live birth number is increased; the patient clinic pregnancy rate is improved; the abortion rate is reduced.

Method of analyzing uterine contractions by analyzing uterine images using deformable model networks in support of embryo transfer techniques. The method is also used to diagnose premature uterine contractile activity in mammals. The method can be used to control contractile activity during embryo transfer or premature labor when used in conjunction with oxytocin antagonists.

The invention relates to an embryo transplantation tube which comprises an outer tube, a heat insulation tube and an embryo tube. The heat insulation tube is of an open or closed structural form. The heat insulation tube comprises a heating layer and a heat insulation layer. The outer tube is connected in the heat insulation tube. The outer tube comprises a straight part and a bent part. The bent part and the straight part are integrally formed in one time, one end of the straight part is provided with a fixed bolt, the other end of the straight part is provided with a cervix opening fixing part, and the cervix opening fixing part is movably connected to the straight part of the outer tube, the bent part is marked with a graduated scale, and the peripheral surface of the bent part is sleeved with a sealing cap. The embryo transplantation tube has the advantages that the embryo transplantation tube is low in cost, clinicians and laboratory workers operate the embryo transplantation tube conveniently, the transplanting efficiency is improved, the surgical operation time is shortened, the embryo transplantation tube has a fixing effect and conforms to the human physiological anatomy structure, it is guaranteed that an embryo is in a homoiothermal state before implantation, the embryo implantation rate and pregnancy rate can be improved, and the embryo transplantation tube can be further clinically popularized and applied later.

This invention belongs to animal embryo culture technology, and disclosed culture medium of sheep embryos in vitro containing velvet extracts, and its preparation methods and in vitro embryo cultivation methods, It is primarily about extraction from the velvet antlers, addition of conventional embryo culture fluid, and for cultivation of whole sheep embryo and cut embryo in vitro, and using the medium for transplantation of whole sheep embryo. The problems about the lower development rate of whole sheep embryo in vitro, embryo transfer pregnancy rates, development rate of the cut embryos in vitro were solved with the application of Chinese medicine velvet extract in field of sheep embryos in vitro. Its economy is lower cost and it is facilitated to application in production.

The invention relates to the field of animal husbandry, and particularly discloses a semen diluent for improving the cryopreservation quality of donkey semen, as well as a preparation method and application of the semen diluent. The semen diluent provided by the invention can significantly improve the cryopreservation activity of the semen and prolong the in-vitro preservation time of sperms. Theapplication provided by the invention is simple in operation, low in application cost, significant in effect and free of any harmful effect on animals. The semen diluent can be widely applied to donkey animal semen preservation and related embryo transfer industry and embryo engineering, and has a wide market prospect.