The invention discloses a
methadone hapten, a preparation method of the
methadone hapten, a
methadone antigen, a methadone
monoclonal antibody and application of the methadone
monoclonal antibody. The methadone
hapten has a structure represented by a formula I shown in a drawing; according to the preparation method of the methadone hapten, methadone is reduced into hydroxyl methadone, the hydroxyl methadone is subjected to
esterification reaction with
succinic anhydride, and then, the methadone hapten is prepared; the methadone
antigen is prepared through connecting the methadone hapten with a
protein carrier; the methadone
monoclonal antibody is prepared through applying the methadone
antigen to an animal for immunization and then using a hybridoma technique or
DNA (Deoxyribonucleic Acid) recombination method; and the methadone
monoclonal antibody is applied to the preparation of reagents,
reagent strips or kits for detecting samples. The methadone hapten, the methadone antigen and the methadone
monoclonal antibody have the advantages that the antigen disclosed by the invention has high
immunogenicity, and the immunoreactivity of methadone is preserved; the methadone
monoclonal antibody is excellent in specificity and high in
potency; and a
colloidal gold-labeled methadone monoclonal antibody immunological test strip is high in sensitivity and strong in specificity, is simple, convenient and quick, and can be applied to site detection, and the like.