79 results about "Immunological tests" patented technology

The invention relates to the immune analysis field, in particular relating to a method for performing an immunological test on biomolecules by avidin / streptavidin magnetic composite particles, which takes avidin / streptavidin magnetic composite particles as carriers, and comprises the following steps:, coating biotinylated antibody or antigen, adding a specimen to be tested and enzyme labeled antibody / enzyme labeled antigen / enzyme labeled anti-antibody, adding chromogenic substrate and testing.Biotin and avidin / streptavidin has strong binding capacity, can fix the antigen / antibody labeled by the biotin through the avidin / streptavidin labeled on the surface of the magnetic composite particles so as to use the system to test specific antibody / antigen. The test system has the advantages of high sensitivity, high specificity, high stability, strong adaptability and the like.

The invention provides a method for immunologically detecting an anti-SmD1 antibody IgG on the basis of an enzyme-linked immunosorbent assay principle and a reagent device , which relate to an analysis method used for enzyme-linked immunosorbent assay of the anti-SmD1 antibody IgG independently, individually and disposably, a reagent device and a matched reagent. In the method, a plurality of reagents required in the enzyme-linked immunosorbent assay of the anti-SmD1 antibody IgG can be contained in one analyzing device; and according to the method, relevant immunological tests can be conveniently conducted according to using requirements on the tested project, and better basis is provided for clinical practice.

A plurality of individual single column test elements are provided for use in a clinical testing apparatus. Each test element is defined by a single test column that includes a quantity of a test material, such as gel material or a bead matrix, including a cover strip used to access the contents of the test column. Individual test elements can be stored, retained and dispensed for testing patient samples.

PCT No. PCT / CH97 / 00121 Sec. 371 Date Sep. 4, 1998 Sec. 102(e) Date Sep. 4, 1998 PCT Filed Mar. 21, 1997 PCT Pub. No. WO97 / 35663 PCT Pub. Date Oct. 2, 1997The test kit includes a substrate and, welded or bonded thereto, a plastic sheet having blisters. One of the blisters is shaped as a siphon. The other blisters act as reaction vessels and also serve to receive and store a reagent. The test kit may contain a test strip in one of the blisters. The test kit is particularly suited for carrying out immunological tests, whereby the siphon markedly facilitates the procedure during the washing step.

Provided is a pretreatment liquid in a specimen and a pretreatment method required for accurate and simple inspection for inspecting influenza virus by immune chromatography. A specimen, sampled from an organism for inspecting influenza, such as pituita, phlegm, and a pharynx wiping liquid, is treated by a pretreatment liquid containing a nonionic surface active agent and 0.3M alkali metal ions.

The invention relates to the technical field of blood coagulants, in particular to a blood coagulant for a blood collection container. The blood coagulant is characterized by consisting of a blood wall attachment preventing agent, thrombin, phosphatidyl ethanolamine, silica micropowder and ethanol, wherein the ethanol is a solvent; and the blood wall attachment preventing agent, the thrombin, the phosphatidyl ethanolamine and the silica micropowder are solutes. The blood coagulant can be applied to vacuum blood collection tubes or other non-vacuum blood collection containers. By the blood coagulant, blood collected into the container can be coagulated within 10 minutes at room temperature of between 2 and 35 DEG C; and serum can be subjected to centrifugal separation within 15 to 30 minutes. The blood coagulant has long retention time, little influence on coagulation speed by temperature and no influence on biochemical and immunological test detection indexes including ion ingredients, and is not influenced by the materials of the blood collection containers, so no matter glass tubes or plastic tubes are used, hemolysis is not caused and blood is not attached to the walls.

A test element, for example in the form of an immunological test strip functioning according to the sandwich principle, for the fluorophoric detection of one or more analytes in a sample comprising an analyte detection zone and a combined control and calibration zone. The combined control and calibration zone include a fluorophore and binding partners for the specific binding of reagents labelled with a fluorophore. Furthermore, the invention concerns a method for calibrating an analyte-specific measurement signal, a method for determining the concentration of an analyte in a sample and the use of the test element for calibrating a signal generated in the analyte detection zone of a test element.

An immunodiagnostic test method includes holding a selection of immunological test elements or consumables in one or more containers attached to or positioned in the analyzer and providing random access to any test element therein. The container can hold multiple types of test elements in compartments or slots. Through sensing of a test element position in its slot, the detection mechanism of the invention provides for random access to multiple types of test elements in any sleeve and within a single sleeve, and provides efficient inventory control. The method increases the number of test element types that may be loaded onto an analyzer and maintains fast determination of inventory.

A process is provided for analyzing a specimen of biological material in any of a number of biochemical or immunological tests for an analyte which involves subjecting the specimen to treatment which develops a color correlating to the amount of analyte in the specimen. According to the invention at least one defined color characteristic selected from hue angle, chroma, saturation and lightness of the developed color is measured and the results of that measurement analyzed to determine the presence or concentration of the analyte in the specimen. Particular applications are to the detection of cancerous or pre-cancerous abnormalities from the analysis of lung mucus, throat mucus, cervical mucus or seminal fluid.

The present invention concerns an immunological test for determining NT-proBNP comprising at least two antibodies to NT-proBNP, wherein at least one of the antibodies to NT-proBNP is a monoclonal antibody. One of these antibodies is directed at least against parts of the epitope of NT-proBNP comprising the amino acids 38 to 50. In addition, one of these antibodies is directed at least against parts of the epitope of NT-proBNP comprising the amino acids 1 to 37 or 43 to 76. The epitope recognized by the antibodies can slightly overlap.

An immunodiagnostic test method includes holding a selection of immunological test elements or consumables in one or more containers attached to or positioned in the analyzer and providing random access to any test element therein. The container can hold multiple types of test elements in compartments or slots. Through sensing of a test element position in its slot, the detection mechanism of the invention provides for random access to multiple types of test elements in any sleeve and within a single sleeve, and provides efficient inventory control. The method increases the number of test element types that may be loaded onto an analyzer and maintains fast determination of inventory.

The invention concerns a method for increasing the dynamic measuring range of especially immunological test elements in particular immunological chromatography test strips that can be evaluated optically that are based on specific binding reactions. The invention enables the dynamic measuring range of test elements based on specific binding reagents, especially of immunological test elements to be shifted towards higher analyte concentrations without impairing the lower detection limit. For this purpose it is proposed according to the invention that at least two zones are provided in or on the test element which contain reagents that generate detectable signals of different strengths due to different affinities for the analyte (for example in the case of antibodies that have different affinities for the analyte) or due to different principles of interaction with the analyte or with other reagents involved in the analyte detection (for example antibodies directed against the analyte in one zone and an analyte analogue in another zone). The signals in the at least two zones are used to evaluate the analyte concentration-signal strength relationship and are used to determine the analyte by means of a suitable method (correlation).

The present invention relates to a method for diagnosing Lyme disease in a subject, the method comprising the steps of: (a) obtaining a sample from said subject, (b) contacting said sample with a source of Borrelia antigens and (c) determining the expression level of a pro-inflammatory cytokine in said sample at the end of step (b).

Disclosed is a method for producing an antibody, which comprises the steps of: immunizing a tissue containing a cell to be immunized in vitro in a liquid culture medium containing an antigen and a stimulant; selecting an immunized cell; and producing the antibody from the selected immunized cell. The method enables to produce an antibody in large quantity within a short period, and therefore can contribute to the widespread use of an immunological test.

The invention discloses a methadone hapten, a preparation method of the methadone hapten, a methadone antigen, a methadone monoclonal antibody and application of the methadone monoclonal antibody. The methadone hapten has a structure represented by a formula I shown in a drawing; according to the preparation method of the methadone hapten, methadone is reduced into hydroxyl methadone, the hydroxyl methadone is subjected to esterification reaction with succinic anhydride, and then, the methadone hapten is prepared; the methadone antigen is prepared through connecting the methadone hapten with a protein carrier; the methadone monoclonal antibody is prepared through applying the methadone antigen to an animal for immunization and then using a hybridoma technique or DNA (Deoxyribonucleic Acid) recombination method; and the methadone monoclonal antibody is applied to the preparation of reagents, reagent strips or kits for detecting samples. The methadone hapten, the methadone antigen and the methadone monoclonal antibody have the advantages that the antigen disclosed by the invention has high immunogenicity, and the immunoreactivity of methadone is preserved; the methadone monoclonal antibody is excellent in specificity and high in potency; and a colloidal gold-labeled methadone monoclonal antibody immunological test strip is high in sensitivity and strong in specificity, is simple, convenient and quick, and can be applied to site detection, and the like.