52 results about "Para-influenza viruses" patented technology

The invention discloses a preparation method of a pseudotype virus for nucleic acid detection of 2019-nCoV, and relates to the technical field of biology. The preparation method comprises a method forpreparing standard quality control products which contain but are not limited to a 2019-nCoV nucleotide sequence and are used for nucleic acid detection through a non-integrated slow virus vector system, a method which is used for preparing the standard quality control products for nucleic acid detection, and a method for constructing a non-integrated slow virus through mutating 64-locus amino acid Asp of integrase of slow virus auxiliary plasmid into Asn, and amino acids Arg, Arg and Lys at 262-264 loci into Ala, Ala and His. In the manner of removing elements of promoters and the like of slow viruses, the load quantity of virus vectors can be promoted, a pseudotype virus which can be used for nucleic acid detection and complete flow quality control is constructed, and the preparation method can be used for constructing a pseudotype virus for corona viruses and can also be used for constructing a pseudotype virus for SARS-COV, MERS-COV, influenza viruses and the like.

This disclosure is directed, inter alia, to polynucleotides, polypeptides, vectors, cells and compositions comprising the same, and their use in affecting viral pathogenesis, in particular for influenza viral infection.

Sample pretreatment solutions for influenza virus tests by immunochromatography are described.

Methods detecting influenza virus by immunochromatography using the sample pretreatment solutions and Kits comprising the sample pretreatment solution and an immunochromatographic device are also described.

The present invention provides piperazine derivatives exhibiting high affinity to the stem region (viral membrane proximal part) of influenza hemagglutinin as determined through competition binding and high virus neutralization activity while having low cytotoxity. Furthermore, the present invention relates to pharmaceutical compositions comprising said piperazine derivatives, methods of preparing said piperazine derivatives, as well as said piperazine derivatives for use in medical prevention or treatment, especially for preventing or treating influenza.

The invention relates to an influenza virus carried HCV nucleic acid test quality control product and a preparation method thereof; the quality control product is prepared from HCV conserved gene carried recombinant influenza virus through enlarged culture, inactivation and purification; the preparation method comprises the following steps: (1) constructing a plasmid which is used for rescuing influenza virus and is interpolated with HCV conserved gene in PR8 virus NS gene; (2) co-transfecting 293T cell to the constructed plasmid with plasmids which are respectively used for transcriptional expression of PR8 viruses PB2, PB1, PA, HA, NP, NA and M, so as to rescue and obtain HCV 5' UTR carried recombinant influenza virus; and (3) enlarged-culturing, inactivating and purifying the recombinant influenza virus so as to obtain the quality control product. The quality control product disclosed by the invention can be used for really simulating HCV pathogens and achieving all-around monitoring on HCV detection; and the quality control product is easy in large-scale preparation, low in cost, good in stability, easy in storage and transportation, and good in application prospect.

The invention provides a general nucleic acid detection method for influenza A virus, influenza B virus, influenza C virus and other recently discovered influenza viruses, belonging to the technical field of biology. The general nucleic acid detection method comprises the following three technical main points: 1) primer sequences needed in nucleic acid detection are determined; 2) detection reaction kinds are determined; and 3) a detection reaction system and reaction conditions are determined. The general nucleic acid detection method is applicable to scientific research on influenza viruses and to clinical diagnosis and detection of animals or human beings.

The invention relates to a 96-hole ELISA (enzyme linked immunosorbent assay) plate-immobilized influenza virus imprint fluorescent sensor and application, and belongs to the technical field of biological molecular detection. The 96-hole ELISA plate-immobilized influenza virus imprint fluorescent sensor is specifically characterized in that an influenza virus particle is used as a template moleculeand is immobilized onto a 96-hole ELISA plate, a thin layer of polyaniline is polymerized to imprint the template virus, the template molecule is eluted, and the left two-dimensional imprinting cavity can be used for specifically identifying the target virus; HRP (horse raddish peroxidase) is used as a biological marker and is directly marked onto the surface of the influenza virus immobilized onto an imprinting film, the HRP can effectively catalyze a H2O2-OPDA (hydrogen peroxide and o-phenylenediamine) system to produce sensitive fluorescent effect, and the produced fluorescent intensity has direct relationship with the concentration of the influenza virus, so as to build a novel influenza virus fluorescent sensor; the prepared imprinting polymer film has the characteristic of an artificial antibody, is used for identifying and detecting the influenza virus, and has been successfully applied to detect a serum sample. The 96-hole ELISA plate-immobilized influenza virus imprint fluorescent sensor provides an influenza virus identifying and detection method with high stability, high specificity and high sensitivity.

The invention relates to new use of a small molecule compound, and specifically relates to uses of 5-(1,1-dimethylallyl propyl)-4,4'-dihydroxyl-2-methoxyl chalcone or a pharmaceutically acceptable salt thereof in preparing medicaments for treating hand-foot-mouth disease virus infection related diseases caused by EV71 and the like and medicaments for influenza virus infection related diseases.

The invention relates to a screening and identification method of an influenza virus attenuated live vaccine strain, and belongs to the technical field of biological pharmacy. A method is researched and developed, and the method is used for obtaining an attenuated live vaccine candidate strain by construction and screening of a Mu phage transposon-mediated virus gene high density random insertion mutant library. A technical system for systematic evaluation of the obtained attenuated live vaccine strain is developed. The method can be applied directly to the screening and evaluation of the influenza virus attenuated live vaccine strain, and has wide referential significance for development and development of other virus attenuated live vaccines.

The invention provides an application of taurolidine in virus resistance. Specifically, the invention provides use of the taurolidine or a derivative thereof, a prodrug, a solvate or a pharmaceutically acceptable salt thereof, or a composition containing the taurolidine or the derivative thereof, the prodrug, the solvate or the pharmaceutically acceptable salt thereof in preparation of antiviral drugs. Research results show that the taurolidine can significantly inhibit influenza virus and coronavirus at a cellular level. In addition, in-vivo experiments prove that taurolidine has a remarkable protection effect on lungs and can prolong the survival time of mice infected with influenza viruses or SARS-CoV-2 viruses. Therefore, the taurolidine can be used for preventing and treating lung diseases caused by the influenza virus or the coronavirus. The research result of the application expands the efficacy range of the taurolidine, and provides a research basis and opens up a new direction for research and development of drugs for preventing or treating pulmonary infection caused by the viruses.

The invention discloses a screening method of influenza virus related host gene mutants, and belongs to the technical field of random mutation. In order to screen out host genes related to influenza viruses, the invention provides a screening method. The screening method comprises the following steps: 1) transfecting a human lung cancer cell strain with CAG-tTA plasmids to obtain a Tel-off cell strain of a transcription activator stably expressed and regulated by tetracycline; co-transfecting the Tel-off cell strain with a gene search vector and plasmids that effectively express transposase toobtain a whole-genome pulmonary epithelial cell gene mutation library; 2) infecting the mutation library obtained in the step 1) by using the H1N1-WSN influenza virus; and 3) extracting the genome DNA of the cell strain, resisting the H1N1-WSN influenza virus, obtained in the step 2), and carrying out sequencing by Splinkette PCR to obtain the influenza virus related host gene mutants. The screening method can be used for screening the influenza virus related host gene mutants.