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Screening and identification method of influenza virus attenuated live vaccine strain

An influenza virus and identification method technology, applied in the field of screening and identification of influenza virus live attenuated vaccine strains, can solve problems such as hindering the production and application of influenza virus live attenuated vaccines, prone to reversion mutation, and vaccine reversion, etc. Achieve the effect of shortening the development cycle, reliable results and fast speed

Inactive Publication Date: 2017-07-11
SUZHOU INST OF SYST MEDICINE
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Problems solved by technology

The mutant virus obtained by cold adaptation often has one or several point mutations in the genome of the virus. This mutation is very prone to reverse mutations in the subsequent vaccine production and immunization process, which may cause the vaccine to return to its ancestors and cause disease.
Nevertheless, the existing cold-adapted attenuated vaccines are all developed by relevant foreign units, and my country does not have its own independent intellectual property rights of attenuated vaccine strains, which hinders the development of live attenuated influenza virus vaccines in China to a certain extent. production and application

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  • Screening and identification method of influenza virus attenuated live vaccine strain
  • Screening and identification method of influenza virus attenuated live vaccine strain
  • Screening and identification method of influenza virus attenuated live vaccine strain

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Embodiment Construction

[0045] Below in conjunction with accompanying drawing, the present invention is specifically described and explained by embodiment:

[0046] The specific technical scheme of the novel influenza virus attenuated live vaccine screening and evaluation method established by the present invention is as follows: figure 1 As shown, the specific description is as follows:

[0047] 1. Using Mu phage transposon-mediated random insertion technology to establish a high-density mutation library of M gene

[0048] First, insert 5'-NNNNNTGCGGCCGCA-3 between each base of the influenza virus A / WSN / 1933M gene according to the operating steps of the Mu phage transposon-mediated random insertion mutagenesis kit (MGS kit, Finnzymes) from Finnzymes Company 'This 15nt long oligonucleotide sequence, thereby obtaining the high-density mutation library of influenza virus M gene (such as figure 1 A; figure 2 ).

[0049] 2. Obtain virus mutant library through influenza virus reverse genetics technol...

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Abstract

The invention relates to a screening and identification method of an influenza virus attenuated live vaccine strain, and belongs to the technical field of biological pharmacy. A method is researched and developed, and the method is used for obtaining an attenuated live vaccine candidate strain by construction and screening of a Mu phage transposon-mediated virus gene high density random insertion mutant library. A technical system for systematic evaluation of the obtained attenuated live vaccine strain is developed. The method can be applied directly to the screening and evaluation of the influenza virus attenuated live vaccine strain, and has wide referential significance for development and development of other virus attenuated live vaccines.

Description

technical field [0001] The invention relates to a method for screening and identifying influenza virus attenuated live vaccine strains, belonging to the technical field of biopharmaceuticals. Background technique [0002] Influenza is a respiratory infectious disease that seriously threatens human health and public health safety. The main method of preventing and treating influenza at present is that patients apply antiviral drugs to treat and normal population is immunized. However, due to the general drug resistance of influenza viruses that have been circulating in recent years, the curative effect of currently used anti-influenza virus drugs is very limited, and the treatment of influenza faces the situation that there is no drug available. Therefore, vaccination has become the most basic and effective means in the current influenza prevention and control system. There are two kinds of influenza vaccines currently in use, one is an inactivated influenza virus vaccine, ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68
CPCC12Q1/04C12Q1/6869C12Q2535/122C12Q2531/113
Inventor 程根宏
Owner SUZHOU INST OF SYST MEDICINE
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