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New use of 5-(1,1-dimethylallyl propyl)-4,4'-dihydroxyl-2-methoxyl chalcone

A technology of methoxychalcone and dimethylallyl, which is applied in the field of chemical drugs 5-4 to achieve the effect of inhibiting infection replication, reducing mouse death, and improving survival rate

Inactive Publication Date: 2013-11-27
INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But whether 5-(1,1-dimethylallyl)-4,4'-dihydroxy-2-methoxychalcone has anti-EV71 virus and influenza virus activity, there is no relevant literature report at present

Method used

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  • New use of 5-(1,1-dimethylallyl propyl)-4,4'-dihydroxyl-2-methoxyl chalcone
  • New use of 5-(1,1-dimethylallyl propyl)-4,4'-dihydroxyl-2-methoxyl chalcone
  • New use of 5-(1,1-dimethylallyl propyl)-4,4'-dihydroxyl-2-methoxyl chalcone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] This embodiment mainly illustrates that 5-(1,1-dimethylallyl)-4,4'-dihydroxy-2-methoxychalcone and its sodium salt are effective in RD cells and Vero cells against EV71 virus infection. Cellular virus activity.

[0026] Materials and Methods

[0027] 1. Cell, Virus and Drug Configuration

[0028] The EV71 virus strain is the international standard strain BrCr strain, which is amplified by infecting RD cells, then mixed, subpackaged and stored at -70°C for future use. Both RD cell and Vero cell culture medium are DMEM (GIBCO) medium containing 10% fetal bovine serum. A maintenance solution containing 2% fetal bovine serum was used for viral infection and cytopathic assays. 5-(1,1-Dimethallyl)-4,4'-dihydroxy-2-methoxychalcone was dissolved in DMSO to 10 mM, and stored at -20°C after aliquoting. The sodium salt of 5-(1,1-dimethylallyl)-4,4'-dihydroxy-2-methoxychalcone is 5-(1,1-dimethylallyl)-4 , 4'-dihydroxy-2-methoxychalcone was dissolved in NaOH solution and adjus...

Embodiment 2

[0041] This example mainly illustrates that 5-(1,1-dimethylallyl)-4,4'-dihydroxy-2-methoxychalcone has a significant effect of inhibiting EV71 virus replication in RD cells and Vero cells .

[0042] Materials and Methods

[0043] 1. Cell, Virus and Drug Configuration

[0044] With embodiment 1.

[0045] 2. Detection of inhibitory effect of 5-(1,1-dimethylallyl)-4,4'-dihydroxy-2-methoxychalcone on EV71 virus RNA

[0046] RD cells and Vero cells were cultured in DMEM medium (GIBCO) containing 10% fetal bovine serum in a 37°C, 5% CO2 incubator. It was observed that the cells were in good growth state. After culturing to the logarithmic growth phase, the cells were inoculated into 96-well cell culture plates at 8,000-10,000 cells / well, and 75-80% of the cells were confluent the next day. Dilute 5-(1,1-dimethylallyl)-4,4'-dihydroxy-2-methoxychalcone with DMEM to 20 μM for use. Replace the 96-well plate of DMEM medium (GIBCO) containing 10% fetal bovine serum with 75-80% RD ce...

Embodiment 3

[0061] This example mainly illustrates the protective effect of 5-(1,1-dimethylallyl)-4,4'-dihydroxy-2-methoxychalcone on mice in EV71 virus infection mouse model.

[0062] Materials and Methods

[0063] 1. ICR suckling mice, viruses and drugs

[0064] Animals were 7-day-old ICR suckling mice. The virus strain is BrCr international standard strain. The drug was dissolved in DMSO to 200 mg / ml, and diluted to 2 mg / ml with PBS when administered.

[0065] 2. Experimental grouping

[0066] They were divided into 3 groups: 5-(1,1-dimethylallyl)-4,4'-dihydroxy-2-methoxychalcone 20mg / kg / d intraperitoneal administration group, EV71 virus infection group group, normal saline control group. 12 mice per group.

[0067] 3. Modeling method

[0068] The EV71 virus was diluted 10-fold with normal saline and infected suckling mice by intraperitoneal route, 30 μl per mouse.

[0069] 4. Administration and observation

[0070] Administration 4h after infection, 5-(1,1-dimethylallyl)-4,4'-...

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Abstract

The invention relates to new use of a small molecule compound, and specifically relates to uses of 5-(1,1-dimethylallyl propyl)-4,4'-dihydroxyl-2-methoxyl chalcone or a pharmaceutically acceptable salt thereof in preparing medicaments for treating hand-foot-mouth disease virus infection related diseases caused by EV71 and the like and medicaments for influenza virus infection related diseases.

Description

Technical field: [0001] The present invention relates to the new application of chemical drug 5-(1,1-dimethylallyl)-4,4'-dihydroxy-2-methoxychalcone, specifically 5-(1,1- Dimethallyl)-4,4'-dihydroxy-2-methoxychalcone in the preparation of treatment and prevention of EV71 (Enterovirus 71) virus (Human enterovirus71) infection and influenza virus (Influenza Virus) infection new uses in medicines. Background technique [0002] Enterovirus 71 (EV71) is a member of the Enterovirus genus of the Picornaradae family and belongs to human Enterovirus A. EV71 can lead to large-scale outbreaks, which may be accompanied by severe CNS complications or fatal pulmonary edema, and its infection hazard is very serious. Currently, only broad-spectrum antiviral drugs such as ribavirin are available for anti-EV71 virus treatment, but the antiviral efficacy is general. Therefore, it is imperative to research and develop effective methods for preventing and controlling EV71 virus. [0003] Inf...

Claims

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Application Information

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IPC IPC(8): A61K31/12A61P31/14A61P31/16
Inventor 王升启杨静伯晓晨韩明明齐向云李康
Owner INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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