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General nucleic acid detection method for influenza viruses

A technology of influenza virus and influenza B virus, applied in the direction of biochemical equipment and methods, microbial determination/inspection, etc.

Inactive Publication Date: 2015-07-08
CHINA ANIMAL HEALTH & EPIDEMIOLOGY CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, various nucleic acid detection methods for influenza virus at home and abroad are only for one type or one subtype of influenza virus, and there is no general nucleic acid detection method for all types of influenza virus

Method used

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Examples

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Embodiment Construction

[0008] The following examples illustrate the technical solution of the present invention, but the protection scope of the present invention is not limited to this example.

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Abstract

The invention provides a general nucleic acid detection method for influenza A virus, influenza B virus, influenza C virus and other recently discovered influenza viruses, belonging to the technical field of biology. The general nucleic acid detection method comprises the following three technical main points: 1) primer sequences needed in nucleic acid detection are determined; 2) detection reaction kinds are determined; and 3) a detection reaction system and reaction conditions are determined. The general nucleic acid detection method is applicable to scientific research on influenza viruses and to clinical diagnosis and detection of animals or human beings.

Description

technical field [0001] The invention belongs to the field of biotechnology; specifically, the invention establishes a general detection method for a group of important viruses (ie, influenza viruses), which is used for the detection of three types of influenza viruses such as A, B, and C, and some newly discovered influenza viruses. Detection has great application value in scientific research, medical clinical diagnosis and veterinary clinical diagnosis. Background technique [0002] Influenza viruses are important pathogens of humans, poultry, horses, pigs and other animals, and can be divided into three types: A, B, and C. Influenza A virus can also be divided into H1N1, H3N2, H5N8, H9N2 and other subtypes. Recently, the influenza virus isolated from cattle is significantly different from the three types of influenza A, B, and C, so it can be regarded as a new type of influenza virus. [0003] Viral nucleic acid detection is one of the important methods for virus detec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68
CPCC12Q1/70C12Q1/6844C12Q2531/113
Inventor 陈继明王素春
Owner CHINA ANIMAL HEALTH & EPIDEMIOLOGY CENT
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