The invention discloses primers, probes and a kit for real-time fluorescent quantitative PCR (polymerase chain reaction) detection of bovine epidemic diseases. The kit at least comprises primers and probes for detecting BHV (Bovine herpesvirus), BPIV (bovine parainfluenza virus), SC type MmmSC (Mycoplasma mycoides subsp mycoides SC), BRSV (Bovine respiratory syncytial virus), BTV (bluetongue virus), BVDV (bovine viral diarrhea virus), BRV (bovine rotavirus), PPRV (peste des petits ruminants virus), RPV (Rinderpestvirus), VSV (vesicular stomatitis virus), FMDV (foot-and-mouth disease virus) and IC. Compared with the prior art, the four TaqMan multiple real-time fluorescent quantitative PCR systems of BHV, BPIV, MmmSC and IC, BRSV, BVDV, BTV and IC, BRV, PPRV, RPV and IC, FMDV, VSV and IC are constructed, and the specificity, repeatability and sensitivity of the systems all reach expected effects, so that the 11 pathogens can be rapidly detected at the same time.