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Application of compound to preparation of drug for treating and preventing novel coronaviruses and influenza viruses

A technology of antiviral drugs and compounds, applied in antiviral agents, drug combinations, pharmaceutical formulas, etc., can solve problems such as limiting the range of efficacy of taurolidine, and achieve the effect of prolonging survival time

Inactive Publication Date: 2021-04-13
ACAD OF MILITARY SCI PLA CHINA ACAD OF MILITARY MEDICAL SCI INST OF MILITARY VETERINARY MEDICINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Due to the current research on the treatment of taurolidine is focused on anti-tumor and antibacterial aspects, the scope of efficacy of taurolidine is limited

Method used

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  • Application of compound to preparation of drug for treating and preventing novel coronaviruses and influenza viruses
  • Application of compound to preparation of drug for treating and preventing novel coronaviruses and influenza viruses
  • Application of compound to preparation of drug for treating and preventing novel coronaviruses and influenza viruses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The impact of embodiment 1 taurolidine on cell safety

[0033] MDCK, Vero-E6, and F81 cells were stored in liquid nitrogen, taken out and resuscitated, passed three generations continuously, and used for experimental research after the cells grew well. MDCK, Vero-E6, and F81 cells were inoculated into 96-well plates, and the amount of cells per well was 1×10 5 , at 37°C, 5% CO 2Culture overnight in a constant temperature cell incubator. When the cell density is 60-70%, add 2% FBS DMEM containing different concentrations of taurolidine (initial concentration 4mg / ml, the drug to be tested is diluted with a 10-fold gradient, a total of 10 concentrations) to maintain 100 μL / well, each concentration was measured in 4 replicate wells. In addition, a blank cell control and a PBS control were set up for 4 repetitions, and the culture was continued, and the state of the cells was observed under a microscope every day. On the second day of dosing, add 10 μL of MTT solution (5...

Embodiment 2

[0035] Embodiment 2 Taurolidine inhibits the detection result of influenza virus infectivity

[0036] MDCK cells were stored in liquid nitrogen, taken out and resuscitated, passed three generations continuously, and used for experimental research after the cells grew well. Place the preserved H3N2 influenza virus strain on ice to slowly thaw, inoculate in a single layer of MDCK cells (not exceeding 24 hours), continue to culture for 72-96 hours, and harvest the virus liquid according to the state of cytopathic disease. And determine its content, with TCID 50 / 100μl is the calculation unit.

[0037] The drug was mixed with the H3N2 subtype influenza virus and introduced into the cells. The well-growing MDCK cells were digested with trypsin to calculate the cell content, and inoculated into a 96-well plate, with the amount of cells per well 1×10 5 . Drug effect studies were performed within 12 hours of seeding and when the cells were in a monolayer state. Inoculation virus ...

Embodiment 3

[0041] Embodiment 3 taurolidine suppresses the detection result of pseudorabies virus infectivity

[0042] Virus: Pseudorabies virus is a DNA virus with an envelope, and the diameter of the virus particle is 150nm-180nm.

[0043] MDCK cells were stored in liquid nitrogen, taken out and resuscitated, passed three generations continuously, and used for experimental research after the cells grew well. Place the preserved viral pseudorabies strain with an envelope on ice to slowly thaw, inoculate in a single layer of MDCK cells (not exceeding 24 hours), continue to culture for 72-96 hours, and harvest the virus liquid according to the state of cytopathic disease. And determine its content, with TCID 50 / 100μl is the calculation unit.

[0044] The drug is mixed with the pseudorabies virus and introduced into the cells. The well-growing MDCK cells were digested with trypsin to calculate the cell content, and inoculated into a 96-well plate, with the amount of cells per well 1×10 ...

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Abstract

The invention discloses application of a compound in the aspect of antiviral drugs, and particularly relates to application of taurolidine to a drug for treating and preventing novel coronaviruses or influenza viruses. The drug is a drug for preventing and treating pulmonary diseases caused by viruses. The viruses include RNA viruses such as influenza viruses and coronaviruses. According to the invention, the effect range of the taurolidine is expanded, it is proved that replication of influenza viruses and SARS-CoV-2 can be remarkably inhibited at the cellular level, it is indicated that the taurolidine has a very positive inhibiting effect on the influenza viruses and SARS-CoV-2, and a research basis is provided and a new direction is opened up for preparing the drug for preventing and treating pulmonary infections caused by the influenza viruses and novel coronaviruses. Moreover, It is proved by in-vitro experiments that the taurolidine has a remarkable protection effect on lungs, can prolong the survival time of mice infected with the influenza viruses, and can be used for preventing and treating the pulmonary diseases caused by the influenza viruses.

Description

technical field [0001] The present invention relates to the technical field of compound medicine application, in particular to the application of a compound in the treatment and prevention of novel coronavirus and influenza virus medicine. Background technique [0002] Coronaviruses (Coronaviruses) are a large class of viruses widely existing in nature. In biological classification, coronaviruses belong to Nidovirales, Coronaviridae, and Coronavirus. They are RNA viruses with an envelope and a linear single-strand positive strand genome. The diameter is 80-120nm, and the nucleic acid is non-segmented single-stranded (+) RNA with a length of 27-31kb. Coronavirus is the longest RNA nucleic acid chain among RNA viruses, and contains important structural features unique to positive-strand RNA: that is, there is a methylated "cap" at the 5' end of the RNA chain, and a PolyA "tail" structure at the 3' end. This structure is very similar to eukaryotic mRNA, and it is also an impo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/549A61P31/14A61P11/00A61P31/16
CPCA61K31/549A61P11/00A61P31/14A61P31/16
Inventor 高玉伟苏忠王铁成周彤李元果吕朝相孙伟洋王雪峰周博向海洋冯娜赵永坤杨松涛夏咸柱
Owner ACAD OF MILITARY SCI PLA CHINA ACAD OF MILITARY MEDICAL SCI INST OF MILITARY VETERINARY MEDICINE
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