419 results about "Antigen-antibody reactions" patented technology

Microfluidic cartridges for agglutination reactions are provided. The cartridges include a microfluidic reaction channel with at least two intake channels, one for an antigen-containing fluid and the other for an antibody-containing fluid, conjoined to a reaction channel modified by incorporation of a downstream flow control channel. At low Reynolds Number, the two input streams layer one on top of the other in the reaction channel and form a flowing, unmixed horizontally-stratified laminar fluid diffusion (HLFD) interface for an extended duration of reaction. Surprisingly, the design, surface properties, and flow regime of microfluidic circuits of the present invention potentiate detection of antibody mediated agglutination at the stratified interface. Antigen:antibody reactions involving agglutination potentiated by these devices are useful in blood typing, in crossmatching for blood transfusion, and in immunodiagnostic agglutination assays, for example.

The present invention provides a dendrimer-based biochip, wherein a flow channel through which a solution containing biopolymer molecules is flowed is formed in the substrate of the biochip, a plurality of dendrimer molecules one end of each of which is bound to the walls of the flow channel are formed thereon, and probe biopolymer or antibody molecules are bound to the tips of the dendrimer molecules so that, if the probe biopolymer molecules are bound, then target biopolymer molecules can be captured by means of a complementary combination and, if the antibody molecules are bound, then protein can be extracted by means of antigen-antibody reaction, whereby biopolymers can be retrieved in a highly efficient manner.

This invention relates to the original position cross checking method of a Hence Kong waters disease type Pamela Ci's body and its reagent box. This invention includes the following steps: composing the three oligoribonucleotides probes of the High Sim markings according to the special DNA list design of the Pamela Ci's body's 16S rDNA list in the bacteria taxonogy, this unusual probe crosses and combines with the Pamela Ci's body 16s rRNA of the Hence Kong waters slice up, and then enlarges signal through the antigen antibody reaction, and displays colour through enzyme reaction. This checking method can combine the pathogeny checking with its pathology together; it can analyze the infection rate and infection intension on the stylebook slice when effiently and correctly checking the Pamela Ci's body; and the checking result is sensitive and precisious; and the slice after being checked can be preserved perennially.

The invention discloses a fluorescent test strip based on resonance energy transfer, and a preparation method and application for the fluorescent test strip. The fluorescent test strip comprises a bottom liner, a sample absorption mat, a combining mat, a chromatography membrane and a water-absorbent mat, wherein the sample absorption mat, the combining mat, the chromatography membrane and the water-absorbent mat are sequentially connected with one another closely and are attached to the bottom liner; antibodies marked by fluorescent receptors are attached to the combining mat; a detection line and a calibration line are arranged on the chromatography membrane; the detection line is close to the combining mat; the calibration line is close to the water-absorbent mat; antigens marked by fluorescent substances are attached to the detection line; proteins marked by fluorescent substances are attached to the calibration line; the antigens marked by the fluorescent substances and the antibodies marked by the fluorescent receptors can be combined by specificity of antigen and antibody reaction; and the proteins marked by the fluorescent substances do not react with the antibodies marked by the fluorescent receptors. The fluorescent test strip has the advantages of safety in operation, simplicity, convenience, sensitivity, low cost, speediness and the like, is wide in application range, and can be used for single-item detection or quick detection items such as multiple-item detection.

The present invention relates to a method for detecting cancer, comprising measuring the expression of a polypeptide having a reactivity of binding to an antibody against a CAPRIN-1 protein having an amino acid sequence shown in any one of the even-numbered SEQ ID NOS: 2-30 in the Sequence Listing via an antigen-antibody reaction in a sample separated from a living organism, and, a reagent for detecting a cancer comprising the CAPRIN-1 protein or a fragment thereof, an antibody against the CAPRIN-1 protein or a fragment thereof, or a polynucleotide encoding the CAPRIN-1 protein or a fragment thereof.

A method of immunoassay of H5 subtype influenza A virus by which the virus can be accurately assayed even in cases where a certain level of mutation has occurred in the H5 subtype influenza A virus, and a kit therefor, and a novel anti-H5 subtype influenza A virus monoclonal antibody which can be used for the immunoassay are disclosed. The antibody or an antigen-binding fragment thereof of the present invention undergoes antigen-antibody reaction with hemagglutinin of H5 subtype influenza A virus, and the corresponding epitope of the antibody or an antigen-binding fragment thereof is located in a region other than the receptor subdomain (excluding C-terminal region thereof consisting of 11 amino acids), which antibody or an antigen-binding fragment thereof does not have neutralizing activity against the influenza A virus.

The present invention relates to (1) A reagent for an immunoassay of a target substance existing in a free form and a bound form in a specimen, comprising a latex 1 which is immobilized with a monoclonal antibody 1 for the target substance, and a latex 2 which has a different mean particle size from the latex 1 and is immobilized with a monoclonal antibody 2 having a different recognition site for the target substance from the antibody 1, (2) An immunoassay method comprising reacting the target substance with the reagent of (1) and determining an amount of the substance based on the result of an agglutination reaction among the target substance, the latex1 and the latex2, and (3) A reagent kit comprising a reagent of (1) and a reagent containing an agglutination accelerator for an antigen-antibody reaction.

An object of the present invention is to provide an immunoassay of PSA using an agglutination accelerator, which has an agglutination accelerating effect equal to or stronger than the known agglutination accelerator; hardly generates non-specific turbidity; and hardly generates salting out even in a solution with a high salt concentration. The present invention relates to an immunoassay of a prostate-specific antigen comprising performing an antigen-antibody reaction in the presence of a polymer having a monomer unit derived from a monomer represented by the following general formula [2]: (wherein R<1>-R<3 >are each independently a hydrogen atom or an alkyl group optionally having a hydroxyl group; R<4 >is an alkylene group; R<5 >is an alkylene group optionally having a substituent and optionally having an oxygen atom in a chain; R<6 >is a hydrogen atom or a methyl group, and X is an oxygen atom or a -NH- group), and a kit of reagent for an immunoassay comprising a reagent containing an agglutination accelerator for the immunoassay.

The invention discloses a protein chip kit for detecting inflammatory factors, which comprises a basement membrane (1) and a reactant and a detection agent (2). A plurality of kinds of specific antibodies are fixed on the basement membrane; the specific antibodies and the inflammatory factors can undergo an antigen-antibody reaction and each specific antibody is respectively fixed on the basementmembrane for forming a plurality of independent recognition loci; and the reactant and the detection agent are used for detecting whether substances capable of undergoing the antigen-antibody reaction with the specific antibodies exist in a sample to be detected or not by an array-ELISA method. The invention also discloses a method for preparing the protein chip kit for detecting the inflammatoryfactors, which comprises a step of fixing the specific antibodies on the basement membrane. The kit of the invention adopts protein chip technology, can detect 40 inflammatory factors, overcomes a plurality of shortcomings and has the advantages of low cost, convenient use, high sensitivity and accuracy, high flux, small using amount of samples, capability of being popularized in an ordinary laboratory, massive production and the like.

The invention relates to a method for fast testing human ABO / Rh / MN blood type and a kit used in the method. The method and the testing kit follow the principle of immunochromatography and realize the blood type test by observing whether residual red substances resulted from the agglutination of erythrocytes exist or not after the antigen antibody reaction of blood type substances. The method has simple operation and can avoid various defects in present ordinary blood type testing methods. In the method, the loading amount of tested blood sample is no more than 10 microliter, and the whole test process lasts no more than 2 minutes.

The present invention provides an immunoassay for specifically measuring with high sensitivity PIVKA-II in serum or plasma through antigen-antibody reaction by adding an animal serum containing thrombin and / or an antibody reacting with human fibrin-like related substances to the reagents. The immunoassay of the invention comprises the steps of adding thrombin and / or an antibody reacting with human fibrin-like related substances to the reagents, and measuring PIVKA-II in serum or plasma.

The invention relates to an enzyme immunosensor based on chitosan and nano-gold, and by using the specific combination of the monoclonal antibody of the cell wall of mycobacterium trberculosis with mycobacterium trberculosis, the enzyme immunosensor is applicable to the rapid detection of mycobacterium trberculosis in milk. The characteristic is that the enzyme immunosensor for detecting mycobacterium tuberculosis is prepared by the following steps: modifying the surface of a glassy carbon electrode through electrochemical deposition method by a mixed solution of chitosan gel, nano-gold solution, and goat anti-mouse antibody labeled by alkaline phosphatase; and fixing the mycobacterium trberculosis monoclonal mouse antibody on the surface of the glassy carbon electrode modified by the goat anti-mouse-chitosan-nano-gold film which is labeled by alkaline phosphatase through the specific antigen-antibody reaction. Quantitative analysis is performed by detecting the electric signal changegenerated before and after the incubation reaction of mycobacterium trberculosis and the modified electrode through differential pulse voltammetry. The preparation method of the sensor is simple, andthe sensor has the advantages of high sensitivity, short detection time, and simple operation, and is applicable to the method for the rapid detection of mycobacterium trberculosis.

The present invention provides a protein capable of binding to a female sex hormone, which protein is supplemented with useful properties for measuring, quantifying and concentrating female sex hormones, such as high sensitivity, low cross-reactivity, unlikelihood of being influenced by interfering substances, and unlikelihood of being influenced by solvents. Specifically, the present invention provides a recombinant protein prepared by obtaining various genes for various antibodies against female sex hormones, and modifying, by gene recombination technology, various properties of the original antibody, such as affinity, avidity, cross-reactivity, resistance for interfering substance on antigen-antibody reaction, resistance for interfering substance on enzymatic color developing reaction, resistance for solvent and the like.

Disclosed is a cancer detection method comprising measuring the expression of a polypeptide in a sample separated from a living body, wherein the polypeptide has a reactivity to bind, through an antigen-antibody reaction, to an antibody directed against CAPRIN-1 protein comprising an amino acid sequence depicted in any even-numbered sequence selected from SEQ ID NO:2 to SEQ ID NO:30 shown in the Sequence Listing. Also disclosed is a cancer detection reagent comprising CAPRIN-1 protein or a fragment thereof, an antibody directed against CAPRIN-1 protein or the fragment thereof, or a polynucleotide encoding CAPRIN-1 protein or the fragment thereof.

Methods of fixing and processing tissue and samples on a membrane by using ultrasound radiation as a part of the method are presented. Ultrasound of a frequency in the range of 0.1-50 MHz is used and the sample or tissue receives 0.1-200 W / cm2 of ultrasound intensity. The use of ultrasound allows much shorter times in the methods. Also presented are apparati comprising transducers of one or of multiple heads for producing the ultrasound radiation and further comprising a central processing unit and optionally comprising one or more sensors. Sensors can include those to measure and monitor ultrasound and temperature. This monitoring system allows one to achieve accurate and optimum tissue fixation and processing without overfixation and tissue damage. The system also allows the performance of antigen-antibody reactions or nucleic acid hybridizations to be completed in a very short time while being highly specific and with a very low or no background.

Disclosed is a method of detecting bioproducts using Localized Surface Plasmon Resonance (LSPR) of gold nanoparticles, which can diagnose bioproducts based on changes in the maximum wavelength occurred by an antigen-antibody reaction after immobilization of the gold nanoparticles onto a glass panel. A sensor using such method exhibits high sensitivity, is low in price, and makes quick diagnosis possible, thereby being applicable to various biological fields associated with environmental contaminants, pathogens and the like, as well as diagnosis of diseases. Further, it provides a technology for manufacturing a sensor having higher sensitivity, low price and quick performance, as compared to conventional methods using SPR.

This invention provides a method to monitor a microorganism that causes infectious disease of a laboratory animal by using a micro flow channel chip immobilized with a molecular to be tested such as an antigen or an antibody of the microorganism that causes infectious disease, the method comprises flowing serum or body fluid taken from the laboratory animal through the minute flow channel of the micro flow channel chip and detecting the antigen antibody reaction on the chip. The method of this invention enabled medical inspection of an infectious disease of a laboratory animal and microorganism monitoring of a laboratory animal, by using minute amount of animal serum or body fluid in a closed system rapidly and sensitively.

The invention provides a heat stabilizer for an antigen-antibody reaction in-vitro diagnostic reagent. The heat stabilizer is characterized by comprising a surfactant, a sealant, a preservative, a buffer solution, a stabilizer and inorganic salt. The heat stabilizer can be used for effectively improving the thermal stability of the antigen-antibody reaction in-vitro diagnostic reagent and effectively prolonging the retention time of a kit.

The invention relates to a polyclonal antibody for detecting the residue and the degradation of a CP4-EPSPS protein in a processed food and application thereof. An animal is immunized by using a CP4-EPSPS protein peptide segment of a coupled keyhole limpet hemocyanin (KHL) to obtain antiserum, namely the antibody of the invention. The application comprises a Western blotting and a colloidal gold test strip and has the characteristics of simplicity, practicability, high sensitivity and the like, wherein the Western blotting is used for detecting the residue and the degradation of the CP4-EPSPS protein in food; and test strip technology is used for the rapid detection of the CP4-EPSPS protein in the food. The invention provides a high-practicability method for the safety detection of a genetically modified food, and has a very high practical application value.

The invention relates to a method for detecting microcystin-LR under condition that the end surface of a gold nanorod is self-assembled and mediated by using a Raman spectrum, belonging to the technical field of immunoassay chemistry. In the invention, the end surface of the gold nanorod is respectively coupled with a coating source of the microcystin-LR and an antibody for resisting the microcystin-LR to form a probe of the gold nanorod, and the synthesized gold nanorod probe is utilized to carry out an immune reaction so as to form nano chains due to an antigen antibody reaction on the end surface of the gold nanorod; the difference of the lengths of assembled nano chains ensures that the intensities of Raman signals are different; and the purpose of detecting the content of the MC-LR is achieved through the changes of the Raman signals. The reaction in the invention is carried out in a liquid environment without a cleaning step, and only one reaction step is needed, thereby reaction conditions are simplified, the labor intensity is lightened, and the detection sensitivity is improved.

[SUMMARY][PURPOSE]The invention provides a novel therapeutic agent or prophylactic agent for cognitive disorders.[SOLUTION MEANS]The invention provides an antibody that participates in antigen-antibody reaction specifically with tau protein that has been phosphorylated in the vicinity of Ser413 of SEQ ID NO: 1, and a therapeutic agent or prophylactic agent for cognitive disorders comprising as an active ingredient a peptide that has been phosphorylated in the vicinity of Ser413.

The present invention provides an immunoassay technique which enables efficient detection of antigen-antibody reaction with high sensitivity by a magnetic method using magnetic particles and a SQUID magnetic sensor or sensors. A system based on the technique includes a disk-shaped sample holder which holds on a circle a plurality of sample containers for accommodating marked samples, resulting from marking of samples with magnetic particles by antigen-antibody reaction; rotating means for rotating the holder around its central shaft; magnetizing means for magnetizing the marked samples outside a magnetic shield; and a magnetic sensor for detecting, within the magnetic shield, magnetic fields generated from the marked samples which have been magnetized. By rotation of the holder, areas fixing and holding different ones of the sample containers are successively inserted into the magnetic shield, and the magnetization of the marked samples accommodated in first ones of the sample containers and the detection of magnetic fields generated from the marked samples accommodated in second ones of the sample containers are executed in parallel.

The invention provides a detection kit applied to colloidal gold competition law and a preparation technology thereof. According to the preparation technology, an antigen-antibody reaction with high degree of specificity is combined with an immune colloidal gold chromatography technique, the preparation technology is improved on the basis of the prior art, fluorescent microspheres are introduced to compete together with colloidal gold marked antibodies for antigens of materials to be detected in a sample, and the fluorescent materials are not visible to naked eyes, so that the sensitivity of the competition law colloidal god detection kit is improved.

Provided are a biosensor and a method of fabricating the same. The biosensor has a transistor structure including a gate electrode formed on a substrate, a gate insulating layer formed on the gate electrode, source and drain electrodes formed on the gate insulating layer, and a channel region formed between the source and drain electrodes. Here, the channel region includes an active layer formed of an active polymer sensing an antigen-antibody reaction and a hydrophilic nano particle. The active layer is formed through direct printing, for example, inkjet printing. The biosensor having such a structure can be increased in reactivity between an antigen and an antibody and hydrophilicity to improve the sensor's characteristics, fabricated in a large-area process using direct printing, and further facilitates formation of devices on various substrates formed of, for example, plastic.

The invention discloses a method for determining the differences of protein contents, which comprises the steps that: a. a plurality of kinds of protein antibodies respectively combined with different oligonucleotide sequences synchronously carry out antigen antibody reaction with one kind of proteantigen to be tested; b. the oligonucleotide sequences of the antigen antibody complex in the step a are coupled together through the coupled reaction by using ligase, and the sequences of a ligation product are led to contain a base sequence representing the source of the protein to be tested; c. the ligation product in the step b is taken as a templet, and a base sequence containing the specificity of the source of the protein to be tested is amplified by adopting the amplification reaction; d. the base sequence representing the source of the protein to be tested in the amplification product sequence is determined by adopting the real-time sequencing technology; and e. the sequence determined in the step d is taken as the source of the protein to be tested, signal intensity of each sequence is compared to obtain the relative contents of the protein to be tested in each source. The method can determine the differences of protein expression content from different sources at tone time with low cost.

The invention relates to a preparation method of a collagen suture line and belongs to the technical field of medical suture lines. The collagen suture line disclosed by the invention is a novel suture line, is extracted from small intestines of plateau black goats, is subject to processes such as degreasing, inactivation of viruses, rinsing, purification, straightening, screening, oil tanning and sterilization and is finally placed in protection liquid for sealed storage. The preparation method has the beneficial effects that the collagen suture line prepared by the method is stable in property, good in elasticity, high in flexibility, easy to knot and high in tensile strength and can be completely absorbed by a human body without antigen-antibody reaction, needle eyes or scars; a sutured wound is smoothly healed; the problems such as prolonged unhealing, foreign body reaction, lithogenesis, inflammatory reaction, erythema generation and induration in the current surgical suturing can be avoided; the collagen suture line is completely absorbed without absorption reaction and is not necessary to remove; the infection and the trouble caused by removing the line and the special pain caused by removing the line of a circumcision and the like can be avoided.

The invention relates to a method for quantitatively detecting alpha-synuclein auto-antibodies in human sera, and prepares recombinant human alpha-synuclein as antigens and rabbit anti-human alpha-synuclein polyclonal antibodies as antibodies into a kit, wherein, the quantities of alpha-synuclein auto-antibodies in the sera of patients with Parkinson's disease (PD) and in the sera of normal healthy persons are detected by utilizing the antigen-antibody reaction principle and are compared; and the presence and quantity of alpha-synuclein auto-antibodies in human sera are judged by the kit on the basis of color reaction.

The invention provides an analysis method of immunochemistry and biochemistry, and discloses a vertical current western blotting method for quickly detecting a human immunodeficiency virus-I (HIV-1) antibody in urine, which is particularly suitable for infants and people who are difficult in collecting blood and are unwilling to collect blood. The vertical current western blotting method comprises the following steps: coating HIV-1 antigen to an NC membrane through electrophoresis and electric conversion of lauryl sodium sulfate and polyacrylamide gel, then sticking the film to the bottom of a self-made reaction tank, and combining the reaction tank with a vacuum filtration system. The method enables a urine sample to vertically pass through the NC membrane in a controllable time so as to accelerate the antigen antibody reaction, increase the combination degree of the antigen and the antibody and further improve the detection sensitivity. The method can quickly detect the HIV-1 antibody in the urine, and ensure the HIV-1 infection condition. The method can be used for laboratory diagnosis and confirmation, medicament treatment effect evaluation and the like, is suitable for lower popularization and use, and has large popularization and application values.

The invention provides a chemiluminescence immunoassay assay determination kit for detecting human growth hormone and a preparation method thereof, belonging to the technical field of immunoassay. Thekit contains a magnetic bead suspension liquid coated with streptavidin, acridinium ester labeled GH antibody and biotin labeled GH antibody. The invention also provides a preparation method of the chemiluminescence immunoassay assay determination kit for detecting human growth hormone. For the kit, a sandwich method principle is adopted for quantitatively determining GH in serum or plasma, a biotin-GH antibody compound is added to streptavidin-magnetic bead suspension liquid, a magnetic bead-avidin-biotin-GH compound is formed by the affiliation reaction of biotin and streptavidin, and an antigen-antibody compound is formed by antigen-antibody reaction to form magnetic bead-biotin-GH antibody-GH-GH antibody-acridinium ester. The detection method has the advantages of high sensitivity, strong specificity, good repeatability, wide detection scope and relatively low cost, and can be widely applied to clinical detection.

The invention provides a rubella virus immunoglobulin G and immunoglobulin M (IgG and IgM) antibody joint inspection kit and a preparation method thereof. The kit comprises a sample pad (1), a colloidal gold pad (2), a nitrocellulose membrane (3), a sample absorption pad (4) and a bottom plate (5), wherein the colloidal gold pad is a colloidal gold-labeled rubella virus antigen glass fiber (or a non-woven fabric); and the nitrocellulose membrane is coated with a mouse anti-human IgM antibody and a rubella virus antigen serving as detection lines, and goat anti-mouse IgG serving as a quality control line in turn. Rubella virus IgG and IgM antibodies are detected by specific antigen antibody reaction and a colloidal gold immunochromatography technology and can be jointly detected through one-time operation, so that the operation process is simplified, and the kit has the characteristics of quick response, high sensitivity and the like, is easy to operate and is economical and practical.