Method for detecting microcystin-LR under condition that end surface of gold nanorod is self-assembled and mediated by using Raman spectrum

Abstract

The invention relates to a method for detecting microcystin-LR under condition that the end surface of a gold nanorod is self-assembled and mediated by using a Raman spectrum, belonging to the technical field of immunoassay chemistry. In the invention, the end surface of the gold nanorod is respectively coupled with a coating source of the microcystin-LR and an antibody for resisting the microcystin-LR to form a probe of the gold nanorod, and the synthesized gold nanorod probe is utilized to carry out an immune reaction so as to form nano chains due to an antigen antibody reaction on the end surface of the gold nanorod; the difference of the lengths of assembled nano chains ensures that the intensities of Raman signals are different; and the purpose of detecting the content of the MC-LR is achieved through the changes of the Raman signals. The reaction in the invention is carried out in a liquid environment without a cleaning step, and only one reaction step is needed, thereby reaction conditions are simplified, the labor intensity is lightened, and the detection sensitivity is improved.

Description

technical field [0001] The invention discloses a method for detecting microcystin (MC-LR) based on Raman spectroscopy under the mediation of end face self-assembly of gold nanorods, belonging to the technical field of immunoassay chemistry. Background technique [0002] With the development of economy, sewage containing a large amount of nitrogen and phosphorus nutrients enters lakes and reservoirs, making the water body eutrophic, resulting in abnormal proliferation of algae and the release of secondary metabolites, Algae Toxins, threatening the consumption of human drinking water. Safety and the safety of other organisms in the body of water. Among them, Microcystins (MCs) are the most widely distributed and the most harmful, and their structures are as follows: [0003] [0004] It contains five fixed amino acids and two variable amino acids X, Y. That is, in the above structural formula, where X and Y are Leu and Arg, respectively, is MC-LR. MC-LR is the most commo...

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Problems solved by technology

Instrumental analysis methods mainly include high-performance liquid chromatography (HPLC), mass spectrometry, liquid chromatography-mass spectrometry, etc. Although these methods are sensitive, they require expensive instruments and equipment, professional operators, and relatively high requirements for inspection materials. Further sample pretreatment can be carried out, which can no longer meet the requirements of modern detection for fast, convenient and accurate

The biochemical method is mainly the protein phosphatase inhibition test method, which has the advantage of being fast and can detect a large number of samples in a few hours, but its biggest disadvantage is that specific protein phosphatases are not commercially available and have poor specificity

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