Vertical current western blotting method for quickly detecting human immunodeficiency virus-I antibody in urine

Abstract

The invention provides an analysis method of immunochemistry and biochemistry, and discloses a vertical current western blotting method for quickly detecting a human immunodeficiency virus-I (HIV-1) antibody in urine, which is particularly suitable for infants and people who are difficult in collecting blood and are unwilling to collect blood. The vertical current western blotting method comprises the following steps: coating HIV-1 antigen to an NC membrane through electrophoresis and electric conversion of lauryl sodium sulfate and polyacrylamide gel, then sticking the film to the bottom of a self-made reaction tank, and combining the reaction tank with a vacuum filtration system. The method enables a urine sample to vertically pass through the NC membrane in a controllable time so as to accelerate the antigen antibody reaction, increase the combination degree of the antigen and the antibody and further improve the detection sensitivity. The method can quickly detect the HIV-1 antibody in the urine, and ensure the HIV-1 infection condition. The method can be used for laboratory diagnosis and confirmation, medicament treatment effect evaluation and the like, is suitable for lower popularization and use, and has large popularization and application values.

Description

technical field [0001] The present invention provides an immunochemical and biochemical analysis method—vertical flow immunoblotting method, which is especially suitable for rapid detection of human immunodeficiency virus-type 1 (HIV-1) antibody in urine by infants, those who have difficulty in taking blood and those who are unwilling to take blood vertical flow immunoblotting. Background technique [0002] Since it was discovered in the 1980s, no effective means of prevention and cure have been found for human immunodeficiency virus (HIV) infection. Early diagnosis and early isolation are still the main ways to prevent its further spread. The methods used to detect HIV infection include nucleic acid detection, antibody detection, and viral load detection, among which antibody detection is prescribed by the World Health Organization and the Ministry of Health as the main method of clinical detection because of its simplicity and speed. Western blotting (WB) is an important ...

AI Technical Summary

Problems solved by technology

However, studies have shown that the concentration of HIV-1 antibodies in urine is low, especially the high concentration of p24 antibodies in serum is low, so urine detection technology needs to be improved

[0004] Detecting HIV-1 antibodies in urine samples by traditional western blotting, the tested antibodies diffuse freely horizontally in the reaction buffer, the binding time is long, the efficiency is low, and the sensitivity is far lower than that of blood testing, so it cannot be used as a routine detection method

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