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Rubella virus IgG and IgM antibody joint inspection kit and preparation method thereof

A rubella virus and kit technology, which is applied in the direction of material analysis, measuring device, and instrument by observing the impact on chemical indicators, can solve the problems of cumbersome operation process, not simple and fast enough, and achieve simplified operation process and rapid response. , the effect of simple operation

Inactive Publication Date: 2011-06-29
北京库尔科技有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, the operation process of this method is cumbersome and not simple and fast enough.

Method used

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  • Rubella virus IgG and IgM antibody joint inspection kit and preparation method thereof
  • Rubella virus IgG and IgM antibody joint inspection kit and preparation method thereof
  • Rubella virus IgG and IgM antibody joint inspection kit and preparation method thereof

Examples

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Embodiment 1

[0033] Embodiment 1: the preparation method of rubella virus IgG, IgM antibody joint detection kit

[0034] 1. Preparation of rubella virus antigen

[0035] Carry out routine BHK-21 cell culture. After the cells grow vigorously, inoculate the standard strain of rubella virus in the culture bottle. 2-3 days after the inoculation of the rubella virus strain, cytopathy begins to appear, and when the cytopathy reaches more than 85%, The culture medium and / or diseased cells were collected, washed 3 times with PBS, sonicated after 3 freeze-thaws, differential centrifugation and glycerol gradient centrifugation to purify rubella virus antigen. After measuring the protein content and coating working concentration, add 50% glycerol and store at -20°C for later use.

[0036] 2. Preparation of anti-human IgM monoclonal antibody

[0037] BALB / C mice were immunized with purified high-concentration human IgM, the splenocytes of the immunized mice were fused with mouse myeloma cells, the a...

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Abstract

The invention provides a rubella virus immunoglobulin G and immunoglobulin M (IgG and IgM) antibody joint inspection kit and a preparation method thereof. The kit comprises a sample pad (1), a colloidal gold pad (2), a nitrocellulose membrane (3), a sample absorption pad (4) and a bottom plate (5), wherein the colloidal gold pad is a colloidal gold-labeled rubella virus antigen glass fiber (or a non-woven fabric); and the nitrocellulose membrane is coated with a mouse anti-human IgM antibody and a rubella virus antigen serving as detection lines, and goat anti-mouse IgG serving as a quality control line in turn. Rubella virus IgG and IgM antibodies are detected by specific antigen antibody reaction and a colloidal gold immunochromatography technology and can be jointly detected through one-time operation, so that the operation process is simplified, and the kit has the characteristics of quick response, high sensitivity and the like, is easy to operate and is economical and practical.

Description

technical field [0001] The invention belongs to the technical field of biological applications, in particular to a rubella virus IgG and IgM antibody joint detection kit and a preparation method thereof. Background technique [0002] Rubella virus (rubella virus, RV) belongs to the Togavirus group of Arthropoviruses, and is the pathogenic virus of rubella. It was isolated from the pharyngeal washings of rubella patients by T.H.Weller and F.A.Neva (1962) and P.D.Parkman et al. (1962). Virus particles are pleomorphic, 50-85nm, and coated. The particles contain molecular weight of 2.6-4.0×10 6 RNA (infectious nucleic acid). The greatest threat of rubella virus to public health is its teratogenicity. The primary infection of the mother during pregnancy can lead to intrauterine infection, and its incidence and teratogenicity are closely related to the gestational age at the time of infection, especially the rubella virus infection in the first 3 months can infect the fetus th...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/531G01N21/78
Inventor 李峰陈立柱杨利
Owner 北京库尔科技有限公司
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