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Rabbit hemorrhagic disease virus colloidal gold test strip

A technology for detecting rabbit hemorrhagic disease virus and test strips, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of limitation and high cost, and achieve the effect of enhanced specificity, low cost and strong specificity

Active Publication Date: 2009-09-02
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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AI Technical Summary

Problems solved by technology

Electron microscopy is intuitive, but expensive
RT-PCR method is sensitive and fast, but expensive and has equipment limitations
So far, there is no report on the establishment of RHDV immune colloidal gold test strip method

Method used

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  • Rabbit hemorrhagic disease virus colloidal gold test strip
  • Rabbit hemorrhagic disease virus colloidal gold test strip
  • Rabbit hemorrhagic disease virus colloidal gold test strip

Examples

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Embodiment Construction

[0049] The steps of the concrete technical route that the present invention adopts:

[0050] 1. Preparation and determination of RHDV monoclonal antibody

[0051] 1. Preparation of RHDV monoclonal antibody

[0052] RHDV monoclonal antibody (referred to as monoclonal antibody) hybridoma cell line A3C was recovered. The hybridoma cell line A3C was deposited in the China Center for Type Culture Collection CCTCC on March 24, 2009, address: Wuhan University, Zip Code: 430072, and the deposit number is CCTCC-C200921. Culture to the logarithmic growth phase, the 10 6 Two hybridoma cells were injected into the peritoneal cavity of BALB / c mice that had been sensitized with paraffin oil for one week. When the abdomen of the mice was extremely distended and sluggish, the ascites was extracted, centrifuged, and the fat was discarded.

[0053] 2. Purification of RHDV monoclonal antibody

[0054] Purification of RHDV monoclonal antibody by caprylic acid-ammonium sulfate method: (1) Take...

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Abstract

The invention relates to a rabbit hemorrhagic disease virus(RHDV) colloidal gold test strip, and belongs to the technical field of immune tests of the colloidal gold. One end of a bottom plate of the test strip is a colloidal gold-RHDV polyclonal antibody combination glass fiber film, and an antibody solid-phase cellulose nitrate film is arranged on the middle part of the bottom plate; a RHDV single-resistance test line, namely T line and a goat anti-rabbit IgG control line, namely C line are arranged on the bottom plate; and the other end of the bottom plate is absorbent paper. When a tested sample is carried with the RHDV, the RHDV can specifically combine the anti-RHDV polyclonal antibody labeled by the colloid so as to be identified by the anti-RHDV monoclonal antibody, namely the double-antibody sandwich specific combination happens, so that whether the sample is carried with the RHDV can be tested. The invention also provides a RHDV immunological test method established by utilizing the monoclonal antibody and the polyclonal antibody, which has the advantages of quickness, accuracy, convenience and the like, and the method is particularly suitable for the on-the-spot test and preliminary screening test and has bright prospect.

Description

technical field [0001] The utility model relates to a colloidal gold detection test strip for rabbit hemorrhagic disease virus (RHDV), which belongs to the technical field of colloidal gold immune detection. Background technique [0002] Rabbit hemorrhagic disease (RHD), also known as rabbit plague, is a rabbit infectious disease characterized by acute, highly contagious, and large-scale death caused by rabbit hemorrhagic disease virus (RHDV). Infected rabbits usually die within 48 to 72 hours. The main features are highly infectious, respiratory system hemorrhage, liver necrosis, visceral edema, congestion and hemorrhage and other changes, which have caused huge economic losses to the rabbit industry all over the world. The International Veterinary Bureau listed the disease as a Class B infectious disease, and the No. 96 Order of the Ministry of Agriculture of my country promulgated it as a Class II disease. [0003] At present, the commonly used methods for detecting RHDV...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/569G01N33/558G01N33/552G01N33/532
Inventor 王芳李超美胡波范志宇蔡少平徐为中张则斌何孔旺
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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